A5083089027- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Hematopoietic Stem Cell Transplantation
- Single-cell and spatial transcriptomics
- COVID-19 Clinical Research Studies
- Contact Dermatitis and Allergies
- Quinazolinone synthesis and applications
- Polymer crystallization and properties
- Reproductive System and Pregnancy
- Chronic Lymphocytic Leukemia Research
- Cytokine Signaling Pathways and Interactions
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- biodegradable polymer synthesis and properties
- Polymer Nanocomposites and Properties
- Cancer Immunotherapy and Biomarkers
- Radiopharmaceutical Chemistry and Applications
- Actinomycetales infections and treatment
Istituti di Ricovero e Cura a Carattere Scientifico
2018-2024
Vita-Salute San Raffaele University
2009-2023
San Raffaele University of Rome
1995-2023
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2021-2023
IRCCS Ospedale San Raffaele
1989-2022
International Society for Experimental Hematology
2014
Istituto Nazionale di Fisica Nucleare, Sezione di Milano
2012-2014
The San Raffaele Telethon Institute for Gene Therapy
2003-2010
Iris Vernici (Italy)
2000
University of Bologna
1998
Invariant natural killer T (NKT) cells are a highly conserved subset of lymphocytes expressing semi-invariant cell receptor (TCR), which is restricted to CD1d and specific for the glycosphingolipid antigen α-galactosylceramide. Their ability secrete variety cytokines, in turn modulate activation both innate acquired immune responses, suggests that invariant NKT exert regulatory role mainly via indirect mechanisms. A relevant question whether can directly help B cells. We document here human...
Abstract Invariant (inv)NKT cells are a subset of autoreactive lymphocytes that recognize endogenous lipid ligands presented by CD1d, and suspected to regulate the host response cell stress tissue damage via prompt production cytokines. We investigated invNKT during progression chronic viral hepatitis caused B or C virus infection, major human disease characterized diffused hepatic necroinflammation with scarring fibrotic reaction, which can progress toward cirrhosis cancer. Ex vivo...
Abstract CD1 molecules present lipid antigens to T cells. An intriguing subset of human cells recognize CD1‐expressing without deliberately added lipids. Frequency, distribution, clonal composition, naïve‐to‐memory dynamic transition these self‐reactive remain largely unknown. By screening libraries T‐cell clones, generated from CD4 + or − CD8 double negative (DN) sorted the same donors, and by limiting dilution analysis, we find that frequency is unexpectedly high in both subsets, range...
Abstract Invariant NK T (iNKT) cells are a separate lineage of lymphocytes with innate effector functions. They express an invariant TCR specific for lipids presented by CD1d and their development differentiation rely on unique gene expression program. We asked whether this program includes microRNAs, small noncoding RNAs that regulate posttranscriptionally play key role in the control cellular programs. To aim, we investigated iNKT cell mice which Dicer, RNase III enzyme generates...
T cells that recognize self-lipids presented by CD1c are frequent in the peripheral blood of healthy individuals and kill transformed hematopoietic cells, but little is known about their antigen specificity potential antileukemia effects. We report self-reactive a novel class self-lipids, identified as methyl-lysophosphatidic acids (mLPAs), which accumulated leukemia cells. Primary acute myeloid B cell blasts express CD1 molecules. mLPA-specific efficiently CD1c+ poorly nontransformed...
Abstract Immune reconstitution plays a crucial role on the outcome of patients given T cell-depleted HLA-haploidentical hematopoietic stem cell transplantation (hHSCT) for hematological malignancies. CD1d-restricted invariant NKT (iNKT) cells are innate-like, lipid-reactive lymphocytes controlling infections, cancer, and autoimmunity. Adult mature iNKT divided in two functionally distinct CD4+ CD4− subsets that express NK receptor CD161 derive from thymic CD4+CD161− precursors. We...
Adoptive immunotherapy with T cells engineered tumor-specific cell receptors (TCRs) holds promise for cancer treatment. However, suppressive cues generated in the tumor microenvironment (TME) can hinder efficacy of these therapies, prompting search strategies to overcome detrimental conditions and improve cellular therapeutic approaches. CD1d-restricted invariant natural killer (iNKT) actively participate immunosurveillance by restricting myeloid populations TME. Here, we showed that...
NKT cells are a small subset of T lymphocytes which express an invariant Vα24JαQ TCR and recognize glycolipids presented by CD1d. In adults, have memory phenotype, frequently associated with oligoclonal expansion, NK cell markers, produce T0 cytokines upon primary stimulation. Because these features, regarded as innate immunity. We investigated from cord blood to see how appear in the absence exogenous stimuli. found that present at comparable frequencies adult peripheral mononuclear both...
Colorectal tumours are often densely infiltrated by immune cells that have a role in surveillance and modulation of tumour progression but burdened immunosuppressive signals, which might vary from primary to metastatic stages. Here, we deployed multidimensional approach unravel the T-cell functional landscape colorectal cancers (CRC) liver metastases, genome editing tools develop CRC-specific engineered T cells.We paired high-dimensional flow cytometry, RNA sequencing immunohistochemistry...
Abstract Results of an investigation isothermal crystallization and thermal behavior poly(ethylene oxide)/poly(ethyl methacrylate) (PEO/PEMA) blends are reported. The blend composition the temperature strongly influence process from melt melting PEO. addition PEMA to PEO causes a depression in spherulite growth rate, overall kinetic constant, temperature. Experimental data on radial rate G constant K n analyzed by means latest theory. From this analysis it emerges that pure conforms regime I...
Abstract Although atopic allergy affects ≤20% of the total population, relationship between protein structure and immunogenic activity allergens is still largely unknown. We observed that group 5 grass are characterized by repeated structural motifs. Using a new algorithm, TEPITOPE, we predicted promiscuous HLA-DR ligands within motifs Lol p5a allergen from rye grass. In vitro binding studies confirmed characteristics these peptides. Moreover, most were novel T cell epitopes able to...
Abstract The crystallization and the thermal behaviour of thin films isotactic polypropylene/hydrogenated oligo(cyclopentadiene) low molecular weight (iPP/HOCP) are studied using optical microscopy differential scanning calorimetry (DSC). spherulite growth rate, overall rate equilibrium melting temperature iPP decreased by addition HOCP to iPP. This leads hypothesis that form a miscible blend in amorphous phase. is also supported detection single blend‐composition‐dependent glass transition...
In chronic lymphocytic leukemia (CLL), the non-hematopoietic stromal microenvironment plays a critical role in promoting tumor cell recruitment, activation, survival, and expansion. However, nature of cells molecular pathways involved remain largely unknown. Here, we demonstrate that leukemic B lymphocytes induce activation retinoid acid synthesis signaling microenvironment. Inhibition RA-signaling causes deregulation genes associated with adhesion, tissue organization chemokine secretion...
Abstract Conventional MHC-restricted T lymphocytes leave thymus with a naive phenotype and require Ag-dependent stimulation coupled to proliferation acquire effector functions. Invariant (i)NKT cells are subset of considered innate because they display an memory independent TCR by foreign Ags. We investigated the differentiation program followed human iNKT studying from relevant set fetal thymi umbilical cord blood samples. find that have already started activates epigenetic transcriptional...
The molecular cloning of the cDNA encoding for an isoallergenic form Lolp 11, a major rye grass (Lolium perenne) pollen allergen, was performed by polymer- ase chain reaction amplification on mRNA extracted from pollen.The amino acid sequence derived truncated 4 and 5 residues at NH2and COOH-terminal ends, respectively, differed only in one position that previously reported.This expressed Escherichia coli fusion to carboxyl terminus human ferritin H-chain.The molecule produced high yields as...
CD1d-restricted invariant (i)NKT cells are innate-like, lipid-reactive T lymphocytes implicated in the control of infections, cancer and autoimmunity. Our study suggests that reconstitution peripheral iNKT cell compartment, following HLA-haploidentical hematopoietic stem transplantation, associates with leukemia children affected by different hematological malignancies.