A5085942002- Immune Cell Function and Interaction
- Radiation Dose and Imaging
- T-cell and B-cell Immunology
- Occupational and environmental lung diseases
- Pleural and Pulmonary Diseases
- Lung Cancer Diagnosis and Treatment
- Medical Imaging Techniques and Applications
- Immunotherapy and Immune Responses
- Medical Imaging and Pathology Studies
- CAR-T cell therapy research
- Neurotransmitter Receptor Influence on Behavior
- Radioactive Decay and Measurement Techniques
- Neuroscience and Neuropharmacology Research
- Chemical Reactions and Isotopes
- Hematopoietic Stem Cell Transplantation
- Carcinogens and Genotoxicity Assessment
- Amoebic Infections and Treatments
- Mitochondrial Function and Pathology
- Digital Radiography and Breast Imaging
- Treatment of Major Depression
- Alkaline Phosphatase Research Studies
- Brain Metastases and Treatment
- Congenital limb and hand anomalies
- Abdominal Surgery and Complications
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
Vita-Salute San Raffaele University
2010-2022
Istituti di Ricovero e Cura a Carattere Scientifico
2006-2021
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2021
International Society for Experimental Hematology
2014
The San Raffaele Telethon Institute for Gene Therapy
2002-2007
University of Pavia
2006
San Raffaele University of Rome
2006
Gene Therapy Laboratory
2006
Mario Negri Institute for Pharmacological Research
1999-2002
Abstract CD1 molecules present lipid antigens to T cells. An intriguing subset of human cells recognize CD1‐expressing without deliberately added lipids. Frequency, distribution, clonal composition, naïve‐to‐memory dynamic transition these self‐reactive remain largely unknown. By screening libraries T‐cell clones, generated from CD4 + or − CD8 double negative (DN) sorted the same donors, and by limiting dilution analysis, we find that frequency is unexpectedly high in both subsets, range...
T cells that recognize self-lipids presented by CD1c are frequent in the peripheral blood of healthy individuals and kill transformed hematopoietic cells, but little is known about their antigen specificity potential antileukemia effects. We report self-reactive a novel class self-lipids, identified as methyl-lysophosphatidic acids (mLPAs), which accumulated leukemia cells. Primary acute myeloid B cell blasts express CD1 molecules. mLPA-specific efficiently CD1c+ poorly nontransformed...
Adoptive immunotherapy with T cells engineered tumor-specific cell receptors (TCRs) holds promise for cancer treatment. However, suppressive cues generated in the tumor microenvironment (TME) can hinder efficacy of these therapies, prompting search strategies to overcome detrimental conditions and improve cellular therapeutic approaches. CD1d-restricted invariant natural killer (iNKT) actively participate immunosurveillance by restricting myeloid populations TME. Here, we showed that...
The variables that influence priming of human naive CD4+ T cells by dendritic (DC) were dissected in vitro analyzing the response to bacterial superantigen toxicshock syndrome toxin or alloantigens. We show under conditions force DC-T cell interactions a single DC can prime up 20 cells. Moreover, strength antigenic stimulation, as determined numbers, antigen dose, TCR avidity and duration interactions, drives progressive differentiation proliferating from non-effector CCR7+ stage, an...
Objective Patient-specific (unique) tumour antigens, encoded by somatically mutated cancer genes, generate neoepitopes that are implicated in the induction of tumour-controlling T cell responses. Recent advancements massive DNA sequencing combined with robust epitope predictions have allowed their systematic identification several malignancies. Design We undertook unique colorectal cancers (CRCs) using high-throughput cDNAs expressed standard cultures, and related stem/initiating cells...
Abstract : Fluoxetine at 10 and 25 mg/kg increased (167 205%, respectively) the extracellular dopamine concentration in prefrontal cortex, whereas (but not 10) citalopram raised (216%) dialysate dopamine. No compound modified nucleus accumbens. The effect of both compounds on cortical was significantly affected by 300 p ‐chlorophenylalanine (PCPA) (fluoxetine, saline, 235% ; PCPA, 230% citalopram, 179% 181%). PCPA depleted tissue serotonin ~90 50%, respectively, prevented fluoxetine 246%...
Abstract A sizable fraction of T cells expressing the NK cell marker NK1.1 (NKT cells) bear a very conserved TCR, characterized by homologous invariant (inv.) TCR Vα24-JαQ and Vα14-Jα18 rearrangements in humans mice, respectively, are thus defined as inv. NKT cells. Because human recognize mouse CD1d vitro, we wondered whether Vα24 could be selected vivo ligands presented CD1d, thereby supporting development mice. Therefore, generated transgenic (Tg) mice chain all The expression Cα−/−...
Abstract Acute leukemia relapsing after chemotherapy plus allogeneic hematopoietic stem cell transplantation can be treated with donor-derived T cells, but this is hampered by the need for donor/recipient MHC-matching and often results in graft-versus-host disease, prompting search new donor-unrestricted strategies targeting malignant cells. Leukemia blasts express CD1c antigen-presenting molecules, which are identical all individuals expressed only mature leukocytes, recognized clones...
Infection by 'high-risk' human papillomaviruses (HPV) is associated with the development of neoplastic lesions. HPV-18 responsible for a very aggressive form cancer and poor survival. As other HPV types, immune surveillance has probably role in control infection. However, little known on immunogenicity. CD4(+) T cells from 16 healthy donors were tested ex vivo reactivity to synthetic peptides corresponding 3 sequences E6 transforming protein predicted bioinformatics as promiscuous HLA-DR...
Abstract Purpose: Renal cell carcinoma (RCC) is considered immunogenic; nonetheless, rare tumor-associated antigens have been identified or are expressed in RCC. Peptidome (i.e., the total content of natural peptides whole cells) from other tumors, such as melanoma, has proved to be immunogenic. The aims this study were determine whether peptidome RCC immunogenic and it contains tumor shared among allogenic RCCs. Experimental Design: Autologous dendritic cells pulsed with used activate vitro...
EARLY DIAGNOSIS OF CONGENITAL DYSPLASIA THE HIP; NEW ROENTGENOLOGIC SIGNSCLAUDIO GARAVAGLIA, M.D.Audio Available | Share