A5091329805- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- MicroRNA in disease regulation
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Histone Deacetylase Inhibitors Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Diet and metabolism studies
- Advanced Breast Cancer Therapies
- Biochemical Acid Research Studies
- Pharmacology and Obesity Treatment
- Epigenetics and DNA Methylation
- Retinoids in leukemia and cellular processes
- Acute Myeloid Leukemia Research
- Sepsis Diagnosis and Treatment
- Breast Cancer Treatment Studies
- Eicosanoids and Hypertension Pharmacology
- PI3K/AKT/mTOR signaling in cancer
- Diabetes Management and Research
- Single-cell and spatial transcriptomics
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
Vita-Salute San Raffaele University
2009-2022
Istituti di Ricovero e Cura a Carattere Scientifico
2021-2022
IRCCS Ospedale San Raffaele
2008-2022
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2021
Université de Strasbourg
2018
Centre National de la Recherche Scientifique
2018
University of Oslo
2018
Oslo University Hospital
2018
Mossakowski Medical Research Institute, Polish Academy of Sciences
2018
Polish Academy of Sciences
2018
B cells require MHC class II (MHC II)-restricted cognate help and CD40 engagement by CD4(+) T follicular helper (T(FH)) to form germinal centers long-lasting Ab responses. Invariant NKT (iNKT) are innate-like lymphocytes that jumpstart the adaptive immune response when activated CD1d-restricted lipid α-galactosylceramide (αGalCer). We previously observed immunization of mice lacking II(-/-)) elicits specific IgG responses only protein Ags mixed with αGalCer. In this study, we investigated...
Abstract Invariant NK T (iNKT) cells are a separate lineage of lymphocytes with innate effector functions. They express an invariant TCR specific for lipids presented by CD1d and their development differentiation rely on unique gene expression program. We asked whether this program includes microRNAs, small noncoding RNAs that regulate posttranscriptionally play key role in the control cellular programs. To aim, we investigated iNKT cell mice which Dicer, RNase III enzyme generates...
Activated lymphocytes release nano-sized vesicles (exosomes) containing microRNAs that can be monitored in the bloodstream. We asked whether elicitation of immune responses is followed by lymphocyte-specific microRNAs. found that, upon activation vitro, human and mouse down-modulate intracellular miR-150 accumulate it exosomes. In vivo, levels increased significantly serum humans immunized with flu vaccines mice ovalbumin, this increase correlated elevation antibody titers. Immunization...
Adoptive immunotherapy with T cells engineered tumor-specific cell receptors (TCRs) holds promise for cancer treatment. However, suppressive cues generated in the tumor microenvironment (TME) can hinder efficacy of these therapies, prompting search strategies to overcome detrimental conditions and improve cellular therapeutic approaches. CD1d-restricted invariant natural killer (iNKT) actively participate immunosurveillance by restricting myeloid populations TME. Here, we showed that...
Significance CD1d-restricted invariant natural killer T (iNKT) cells are innate-like lymphocytes that play fundamental roles in cancer, autoimmunity, and infections. iNKT acquire effector functions already the thymus, because of a distinct developmentally regulated genetic program is critically controlled by miRNAs. Our study unveils unexpected requirement for miRNA-dependent fine-tuning TGF-β signaling control cell development functional differentiation. The targeting lineage-specific...
Objectives: Inhibition of histone deacetylase activity is one the epigenetic mechanisms in regulation cellular gene expression. We investigated antitumor effect HA-But, a new inhibitor, which hyaluronic acid esterified with butyric residues and selectively bind to CD44, overexpressed most human cancers, including pancreatic cancer. Methods: analyzed HA-But on expression level some cell cycle (p21waf1/cip1, p27kip1, p53, cyclin D1), apoptosis (BAX, caspase-7, Bcl-2, survivin),...
Abstract NKT17 cells represent a functional subset of Vα14 invariant NKT (iNKT) with important effector functions in infections and autoimmune diseases. The mechanisms that drive cell differentiation the thymus are still largely unknown. percentage has high variability between murine strains due to differential thymic differentiation. For example, NOD strain carries absolute numbers compared other strains. In this study, we used mouse model analyze what regulates frequency peripheral...
Upon activation, lymphocytes release vesicles containing microRNAs (miRNAs). However, little is known as to whether this results in modulation of circulating miRNAs (the miRNome) the serum. The present work aims identify lymphocyte subset-specific signatures within serum miRNome. We therefore assessed miRNA expression profiles wild-type mice; mice lacking either CD4(+) T cells, CD8(+) invariant natural killer (iNKT) or B cells; and, a control, which Dicer has been ablated lymphocytes. found...
To assess the effectiveness, safety and feasibility of revised, simplified nurse-managed version our insulin infusion protocol, adapted to new recommended glycaemic target 140 180 mg/dL (Desio Diabetes Diagram i.v. 140–180). All clinical responses Desio 140–180 in use for 3 years were recorded patients with diabetes or hyperglycaemia admitted intensive cardiac care unit. feasibility, we asked nurses complete an ad hoc questionnaire anonymously when protocol had been 2 years. From December...
MicroRNAs (miRNAs) are single-stranded RNA that have key roles in the development of immune system and involved pathogenesis various autoimmune diseases. We previously demonstrated two members miR106b-25 cluster miR17-92 paralog were upregulated T regulatory cells from multiple sclerosis (MS) patients. The aim present work was to clarify impact clusters MS pathogenesis. Here, we show mice lacking or both (double knockout) protected Experimental Autoimmune Encephalomyelitis (EAE) while...
Invariant Natural Killer T (iNKT) cells are innate-like Lymphocytes expressing a conserved semi-invariant cell receptor (TCR) specific for self or microbial lipid antigens presented by the non-polymorphic MHC class I-related molecule CD1d. Preclinical and clinical studies support role iNKT in cancer, autoimmunity infectious diseases. very throughout species their investigation has been facilitated mouse models, including CD1d-deficient iNKT-deficient mice, possibility to unequivocally detect...
The data described in this article are related to "High levels of eukaryotic Initiation Factor 6 (eIF6) required for immune system homeostasis and steering the glycolytic flux TCR-stimulated CD4+ T cells both mice humans" (Manfrini et al., press) [1]. eIF6 is a translation initiation factor ribosomal biogenesis (Sanvito 1999) [2] proper translational (Gallo Manfrini, 2015; Miluzio 2016) [3], [4] whose protein abundance requires tight regulation. Here we analyze by flow cytometry effects...
We describe a multi-step high-dimensional (HD) flow cytometry workflow for the deep phenotypic characterization of T cells infiltrating metastatic tumor lesions in liver, particularly derived from colorectal cancer (CRC-LM). First, we applied novel cytometer setting approach based on single positive rather than fluorescent beads, resulting optimal sensitivity when compared with previously published protocols. Second, set up 26-color antibody panel designed to assess functional state both...
miR-21 is highly expressed in iNKT and activated T cells, but its T-cell autonomous functions are poorly defined. We sought to investigate the role of development representing adaptive innate-like populations, respectively.We studied mice with a conditional deletion all mature lymphocytes.Thymic peripheral compartments were normal KO mice. Upon activation vitro, depletion reduced survival, TH17 polarisation and, remarkably, T- cell ability respond low-affinity antigens, without altering...
Invariant Natural Killer T (iNKT) cells are innate-like Lymphocytes expressing a conserved semi-invariant cell receptor (TCR) specific for self or microbial lipid antigens presented by the non-polymorphic MHC class I-related molecule CD1d. Preclinical and clinical studies support role iNKT in cancer, autoimmunity infectious diseases. very throughout species their investigation has been facilitated mouse models, including CD1d-deficient iNKT-deficient mice, possibility to unequivocally detect...