A5084664500- Genetics, Aging, and Longevity in Model Organisms
- bioluminescence and chemiluminescence research
- Mitochondrial Function and Pathology
- Bacterial biofilms and quorum sensing
- Microbial Community Ecology and Physiology
- Chemical Reactions and Isotopes
- Bacteriophages and microbial interactions
- Photoreceptor and optogenetics research
- Spaceflight effects on biology
- Birth, Development, and Health
- Circadian rhythm and melatonin
- Tryptophan and brain disorders
- Biochemical Acid Research Studies
- Animal Genetics and Reproduction
- Cancer Research and Treatments
- Biofield Effects and Biophysics
- Fecal contamination and water quality
- Origins and Evolution of Life
- Pancreatic function and diabetes
- Anesthesia and Neurotoxicity Research
- Open Education and E-Learning
- Neuroethics, Human Enhancement, Biomedical Innovations
- Virus-based gene therapy research
- Adipose Tissue and Metabolism
- Diet and metabolism studies
University of Aberdeen
2001-2016
Institute of Medical Sciences
2015
The ATP levels of an organism are important physiological parameter that is affected by genetic make up, ageing, stress and disease.We have generated luminescent C. elegans through ubiquitous, constitutive expression firefly luciferase, widely used for in vitro determination. We hypothesise whole animal luminescence reflects its intracellular vivo. To test this, we characterised the bioluminescence response during sublethal exposure to, recovery from azide, a treatment inhibits mitochondrial...
Abstract Mitochondria are a target of many drugs and environmental toxicants; however, how toxicant‐induced mitochondrial dysfunction contributes to the progression human disease remains poorly understood. To address this issue, in vivo assays capable rapidly assessing function need be developed. Here, using model organism Caenorhabditis elegans , we describe assess role electron transport chain, glycolysis, or fatty acid oxidation energy metabolism following toxicant exposure,...
A transgenic strain of the model nematode Caenorhabditis elegans in which bioluminescence reports on relative, whole-organism ATP levels was used to test an environmentally-relevant mixture pollutants extracted from processed sewage sludge. Changes bioluminescence, following exposure sludge extract, were assess relative and overall metabolic health. Reproductive function longevity also monitored. short (up 8 h) sublethal L4 larval stage worms extract had a concentration-dependent,...
We describe a novel approach to assess toxicity the free‐living nematode Caenorhabditis elegans that relies on ability of firefly luciferase report endogenous ATP levels. have constructed bioluminescent C. with luc gene under control constitutive promoter. Light reduction was observed in response increasing temperature, concentrations copper, lead and 3,5‐dichlorophenol. This due increased mortality coupled decreased metabolic activity surviving animals. The light emitted by transgenic...
Abstract Quantitative models of bacterial conjugation are useful tools in environmental risk assessment and studies the ecology evolution communities. We constructed a mathematical model for gene transfer between bacteria growing on solid surface. The considers that donor recipient cells will form separate colonies, which grow exponentially until nutrient exhaustion. Conjugation occurs when colonies meet, all becoming transconjugants instantly, after they act as donors. was tested...
Sublethal metabolic effects are informative toxicological end points. We used a rapid quantitative point, bioluminescence of firefly luciferase expressing Caenorhabditis elegans, to assess sublethal chronic exposure (19 h) the oxidative stress agent and environmental pollutant cadmium (provided as chloride salt). Bioluminescence declined in concentration-dependent manner concentration range tested (0-30 microM Cd), with comparable sensitivity reproduction developmental assay points (after 67...
This study determined that the bacterial luciferase fusion gene ( lux AB) was not a suitable in vivo reporter model eukaryotic organisms Saccharomyces cerevisiae and Caenorhabditis elegans . Lux AB expressing S. strains displayed distinctive rapid decays luminescence upon addition of substrate, n ‐decyl aldehyde, suggesting toxic response. Growth studies toxicity bioassays have subsequently confirmed, aldehyde substrate to both at concentrations well tolerated by Escherichia coli As is an...
The multicellular model organism Caenorhabditis elegans is a small nematode of approximately 1 mm in size adulthood that genetically and experimentally tractable. It economical easy to culture dispense liquid medium which makes it well suited for medium-throughput screening. We have previously validated the use transgenic luciferase expressing C. strains provide rapid vivo assessment nematode's ATP levels.1-3 Here we present required materials procedure carry out bioassays with...
The multicellular model organism Caenorhabditis elegans is a small nematode of approximately 1 mm in size adulthood that genetically and experimentally tractable. It economical easy to culture dispense liquid medium which makes it well suited for medium-throughput screening. We have previously validated the use transgenic luciferase expressing C. strains provide rapid vivo assessment nematode's ATP levels.1-3 Here we present required materials procedure carry out bioassays with...