A5086981177- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- Pancreatic and Hepatic Oncology Research
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Graphene and Nanomaterials Applications
- Immune cells in cancer
- Chronic Myeloid Leukemia Treatments
- Acute Myeloid Leukemia Research
- SARS-CoV-2 and COVID-19 Research
- Chemokine receptors and signaling
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Chronic Lymphocytic Leukemia Research
- Nanopore and Nanochannel Transport Studies
- Cell Adhesion Molecules Research
- Extracellular vesicles in disease
- Heat shock proteins research
- Virus-based gene therapy research
Affiliated Hospital of Hangzhou Normal University
2024
Houston Methodist
2015-2024
Mirna Therapeutics (United States)
2023-2024
Cornell University
2013-2022
Q Therapeutics (United States)
2022
Weill Cornell Medicine
2016-2021
Methodist Hospital
2012-2021
Wenzhou Medical University
2019
Methodist Hospital
2011-2017
Tianjin Medical University Cancer Institute and Hospital
2013
Abstract Messenger RNA (mRNA) vaccines are being used to combat the spread of COVID-19 (refs. 1–3 ), but they still exhibit critical limitations caused by mRNA instability and degradation, which major obstacles for storage, distribution efficacy vaccine products 4 . Increasing secondary structure lengthens half-life, which, together with optimal codons, improves protein expression 5 Therefore, a principled design algorithm must optimize both structural stability codon usage. However, owing...
The unfolded protein response (UPR) is a cellular homeostatic mechanism that activated in many human cancers and plays pivotal roles tumor progression therapy resistance. However, the molecular mechanisms for UPR activation regulation cancer cells remain elusive. Here, we show oncogenic MYC regulates inositol-requiring enzyme 1 (IRE1)/X-box binding (XBP1) branch of breast via multiple mechanisms. We found directly controls IRE1 transcription by to its promoter enhancer. Furthermore, forms...
The therapeutic resistance of pancreatic ductal adenocarcinoma (PDAC) is partly ascribed to ineffective delivery chemotherapy cancer cells. We hypothesized that physical properties at vascular, extracellular, and cellular scales influence response gemcitabine-based therapy.We developed a method measure mass transport during routine contrast-enhanced CT scans individual human PDAC tumors. Additionally, we evaluated gemcitabine infusion resection in 12 patients, measuring incorporation into...
Effective delivery holds the key to successful in vivo application of therapeutic small interfering RNA (siRNA).In this work, we have developed a universal siRNA carrier consisting mesoporous silica nanoparticle (MSNP) functionalized with cyclodextrin-grafted polyethylenimine (CP).CP provides positive charge for loading through electrostatic interaction and enables effective endosomal escape siRNA.Using intravital microscopy were able monitor tumor enrichment CP-MSNP/siRNA particles live...
mRNA-based vaccines have the benefit of triggering robust anti-cancer immunity without potential danger genome integration from DNA or limitation antigen selection peptide vaccines. Yet, a conventional mRNA vaccine comprising condensed molecules in positively charged protein core structure is not effectively internalized by antigen-presenting cells. It cannot offer sufficient protection for degradation plasma and tissue enzymes either. Here, we developed lipopolyplex that consists...
E-selectin is a surface marker of endothelial cell (EC) inflammation, one the hallmarks atherogenesis. Thus, we tested hypothesis that delivery microRNA (miR)-146a and miR-181b with an E-selectin-targeting multistage vector (ESTA-MSV) to inflamed endothelium covering atherosclerotic plaques inhibits atherosclerosis. Cy5-conjugated miR-146a were packaged in polyethylene glycol-polyethyleneimine (PEG/PEI) nanoparticles loaded into ESTA-MSV microparticles. Both miRs downregulated tumor necrosis...
Pegylation of nanoparticles has been widely implemented in the field drug delivery to prevent macrophage clearance and increase accumulation at a target site. However, shielding effect polyethylene glycol (PEG) is usually incomplete transient, due loss nanoparticle integrity upon systemic injection. Here, we have synthesized unique PEG-dendron-phospholipid constructs that form super stealth liposomes (SSLs). A β-glutamic acid dendron anchor was used attach PEG chain several distearoyl...
Abstract Purpose: Understanding the mechanism of radioresistance could help develop strategies to improve therapeutic response patients with PDAC. The SMAD4 gene is frequently mutated in pancreatic cancer. In this study, we investigated role deficiency cancer cells' radiotherapy. Experimental Design: We downregulated expression siRNA or shRNA and overexpressed mutant cells followed by clonogenic survival assay evaluate their effects on cell radioresistance. To study radioresistance, loss...
Abstract The tumor-promoting potential of CCL5 has been proposed but remains poorly understood. We demonstrate here that an autocrine CCL5–CCR5 axis is a major regulator immunosuppressive myeloid cells (IMC) both monocytic and granulocytic lineages. absence the abrogated generation myeloid-derived suppressor tumor-associated macrophages. In parallel, enhanced maturation intratumoral neutrophils macrophages occurred in spite tumor-derived CCL5. refractory nature ccl5-null precursors to was...
Ruthenium coordination complexes have the potential to serve as novel theranostic agents for cancer. However, a major limitation in their clinical implementation is effective tumor accumulation. In this study, we developed liposome-based nanodelivery system [Ru(phen)
Site-specific localization is critical for improving the therapeutic efficacy and safety of drugs. Nanoparticles have emerged as promising tools localized drug delivery. However, over 90% systemically injected nanocarriers typically accumulate in liver spleen due to resident macrophages that form mononuclear phagocyte system. In this study, clinically approved antimalarial agent chloroquine was shown reduce nanoparticle uptake by suppressing endocytosis. Pretreatment mice with a relevant...
Abstract Immunosuppressive tumor microenvironment (TME) and ascites-derived spheroids in ovarian cancer (OC) facilitate growth progression, also pose major obstacles for therapy. The molecular pathways involved the OC-TME interactions, how crosstalk impinges on OC aggression chemoresistance are not well-characterized. Here, we demonstrate that tumor-derived UBR5, an E3 ligase overexpressed human associated with poor prognosis, is essential progression principally by promoting...
Significance This manuscript describes the identification and characterization of two previously unidentified cancer genes, ribosomal protein L39 myeloid leukemia factor 2, that play an important role in tumor initiation metastasis. Knockdown these genes triple negative breast (TNBC) models significantly reduces primary-tumor growth, as well Mutations are associated with worse survival breast-cancer patients. Both regulated by nitric oxide signaling pathway. Identification represents a...