A5034714015- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Chemical Synthesis and Analysis
- Viral Infections and Immunology Research
Abstract Messenger RNA (mRNA) vaccines are being used to combat the spread of COVID-19 (refs. 1–3 ), but they still exhibit critical limitations caused by mRNA instability and degradation, which major obstacles for storage, distribution efficacy vaccine products 4 . Increasing secondary structure lengthens half-life, which, together with optimal codons, improves protein expression 5 Therefore, a principled design algorithm must optimize both structural stability codon usage. However, owing...
Messenger RNA (mRNA) vaccines are being used for COVID-19, but still suffer from the critical issue of mRNA instability and degradation, which is a major obstacle in storage, distribution, efficacy vaccine. Previous work showed that optimizing secondary structure stability lengthens half-life, which, together with optimal codons, increases protein expression. Therefore, principled design algorithm must optimize both structural codon usage to improve efficiency. However, due synonymous space...