A5048867660- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Mast cells and histamine
- Peroxisome Proliferator-Activated Receptors
- Nanoparticle-Based Drug Delivery
- Neuroscience and Neuropharmacology Research
- Cell Adhesion Molecules Research
- Amino Acid Enzymes and Metabolism
- Eicosanoids and Hypertension Pharmacology
- Inflammatory mediators and NSAID effects
- Polyamine Metabolism and Applications
- Diet, Metabolism, and Disease
- Asthma and respiratory diseases
- Inflammasome and immune disorders
- Pharmacological Receptor Mechanisms and Effects
- S100 Proteins and Annexins
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nitric Oxide and Endothelin Effects
- Liver Disease Diagnosis and Treatment
- Renal Diseases and Glomerulopathies
- Phagocytosis and Immune Regulation
- Adipose Tissue and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Chemical Synthesis and Analysis
- Angiogenesis and VEGF in Cancer
University of Turin
2011-2024
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2004-2016
Boehringer Ingelheim (Germany)
2016
University of Florence
1981-2015
Zero to Three
2010
William Harvey Research Institute
2010
University of Milan
1990-2006
IRCCS Istituto Auxologico Italiano
2006
Istituti di Ricovero e Cura a Carattere Scientifico
2006
Amgen (United States)
1998-2004
Hydrogen sulfide (H2S) is a novel gaseous mediator produced by cystathionine-beta-synthase and cystathionine-gamma-lyase in the cardiovascular system, including heart. Using rat model of regional myocardial ischemia/reperfusion, we investigated effects an H2S donor (sodium hydrogen [NaHS]) on infarct size apoptosis caused ischemia (25 min) reperfusion (2 h). Furthermore, potential mechanism(s) cardioprotective effect(s) afforded NaHS. Specifically, demonstrate that NaHS (1) attenuates...
The generation of endogenous hydrogen sulfide may either limit or contribute to the degree tissue injury caused by ischemia/reperfusion. A total 74 male Wistar rats were used investigate effects and exogenous in renal Administration irreversible cystathionine gamma-lyase (CSE) inhibitor, dL-propargylglycine, prevented recovery function after 45 min ischemia 72 h reperfusion. donor sodium hydrosulfide attenuated (renal, tubular, glomerular) dysfunction 6 Western blot analysis kidneys taken at...
Epidemiological studies pointed out to a strong association between vitamin D deficiency and type 2 diabetes prevalence. However, the role of supplementation in skeletal muscle, tissue that play crucial maintenance glucose homeostasis, has been scarcely investigated so far. On this basis, study aimed evaluate effect murine model diet-induced insulin resistance with particular attention effects evoked on muscle. Male C57BL/6J mice (n = 40) were fed control or High Fat-High Sugar (HFHS) diet...
Background and purpose: Nutrient overload leads to obesity insulin resistance. Pioglitazone, a selective peroxisome proliferator‐activated receptor (PPAR)γ agonist, is currently used manage resistance, but the specific molecular mechanisms activated by PPARγ are not yet fully understood. Recent studies suggest involvement of suppressor cytokine signalling (SOCS)‐3 in pathogenesis This study aimed investigate hepatic pathway activation non‐genetic insulin‐resistant animal model. Experimental...
Mutations in oncogenes and tumor suppressor genes are responsible for tumorigenesis represent favored therapeutic targets oncology. We exploited homologous recombination to knock-in individual cancer mutations the genome of nontransformed human cells. Sequential introduction multiple was also achieved, demonstrating potential this strategy construct progression models. Knock-in cells displayed allele-specific activation signaling pathways mutation-specific phenotypes different from those...
1. Histamine secretion from rat mast cells occurs in the presence of nanomolar concentrations acetylcholine. 2. Intact glycolytic and oxidative metabolism is required for acetylcholine-induced histamine secretion. Removal extracellular glucose, hypoxia, cyanide monoiodoacetate almost completely inhibit 3. The dependent on H ion concentration blocked when are exposed to Na-deficient media. 4. order potency cholinrgic agonists evoking oxotremorine > acetylcholine choline carbamycholine...
The aim of this study was to evaluate the effects chronic treatment with empagliflozin, a potent and selective sodium glucose cotransporter-2 inhibitor, in murine model diet-induced obesity insulin resistance, focusing on drug body weight reduction nucleotide-binding domain, leucine-rich repeat containing protein (NLRP)-3 inflammasome activation, which have never been investigated date. Male C57BL/6 mice were fed control or high fat–high sugar (HFHS) diet for 4 months. Over last 2 months,...
Aim: This study investigates cancer targeted gold nanoparticles as ultrasound sensitizers for the treatment of cancer. Methods: The sensitizer activity folate-PEG decorated (FA-PEG-GNP) has been studied on human cell lines that overexpress folate receptors (KB and HCT-116) another does not (MCF7), at two energy densities (8 × 10-6 J cm-2 8 10-5 cm-2, 5 min 1.866 MHz). Results: FA-PEG-GNP selectively KB HCT-116 cells a remarkable reduction in growth was observed upon exposure, along with...
Abstract Although recent preclinical and clinical studies have demonstrated that recombinant human relaxin (rh RLX ) may important therapeutic potential in acute heart failure chronic kidney diseases, the effects of rh administration against renal ischaemia/reperfusion (I/R) injury never been investigated. Using a rat model 1‐hr bilateral artery occlusion followed by 6‐hr reperfusion, we investigated (5 μg/Kg i.v.) given both at beginning after 3 hrs reperfusion. Acute attenuated functional...
Pyroptosis is a caspase-1-dependent pro-inflammatory form of programmed cell death implicated in the pathogenesis autoinflammatory diseases as well disorders characterized by excessive and inflammation. Activation NLRP3 inflammasome key event pyroptotic cascade. In this study, we describe synthesis chemical tuning α,β-unsaturated electrophilic warheads toward development antipyroptotic compounds. Their pharmacological evaluation structure–activity relationships are also described. Compound 9...
Camptothecin (CPT), a pentacyclic alkaloid, is an inhibitor of DNA Topoisomerase-I and shows wide spectrum anti-cancer activities. The use CPT has been hampered by poor aqueous solubility high degradation rate. Previously, it reported that encapsulated in β-cyclodextrin-nanosponges (CN-CPT) overcomes these disadvantages improves the CPT's inhibitory effect on DU145 prostate tumor cell lines, PC-3 growth vitro. This work extends observations showing CN-CPT significantly inhibits adhesion...
The effect of L‐glutamate (Glu) on human lymphocyte function was studied by measuring anti‐CD 3 monoclonal antibody (mAb) or phytohaemagglutinin (PHA)‐induced intracellular Ca 2+ ([Ca ] i ) rise (Fura‐2 method), and cell proliferation (MTT assay). Glu (0.001 – 100 μ M did not modify basal [Ca , but significantly potentiated the effects mAb PHA. Maximal rises over resting cells were: 165±8 247±10 n at 3.0×10 −2 mg ml −1 mAb; 201±4 266±9 5.0×10 PHA, in absence presence 1 Glu, respectively....
The effects of substance P (SP), neurokinin A (NKA) and B (NKB) were evaluated on superoxide anion ( ) production by guinea‐pig alveolar macrophages (AM). SP dose‐dependently (ED 50 = 0.7 n m evoked from AM; the N‐terminal heptapeptide, SP(1–7), was ineffective. In presence thiorphan (10 −5 ), an enkephalinase inhibitor, stimulating not significantly modified. NKA NKB both able to induce AM, ED values being 0.1 1.3 , respectively. Therefore, rank order activity natural tachykinins > NKB....
There is evidence that insulin reduces brain injury evoked by ischemia/reperfusion (I/R). However, the molecular mechanisms underlying protective effects of remain unknown. Insulin a well-known inhibitor glycogen synthase kinase-3beta (GSK-3beta). Here, we investigate role GSK-3beta inhibition on I/R-induced cerebral in rat model insulinopenic diabetes.Rats with streptozotocin-induced diabetes were subjected to 30-min occlusion common carotid arteries followed 1 or 24 h reperfusion. (2-12...
Rationale: Peroxisome proliferator–activated receptor (PPAR)-β/δ is a transcription factor that belongs to the PPAR nuclear hormone family, but role of PPAR-β/δ in sepsis unknown.Objectives: We investigated murine models LPS-induced organ injury and dysfunction cecal ligation puncture (CLP)–induced polymicrobial sepsis.Methods: Wild-type (WT) knockout (KO) mice C57BL/6 were subjected LPS for 16 hours. received agonist GW0742 (0.03 mg/kg intravenously, 1 h after LPS) or plus antagonist...
Although the molecular links underlying causative relationship between chronic low-grade inflammation and insulin resistance are not completely understood, compelling evidence suggests a pivotal role of nucleotide-binding oligomerization domain (NOD)-like receptor pyrin containing 3 (NLRP3) inflammasome. Here we tested hypothesis that either selective pharmacological inhibition or genetic downregulation NLRP3 inflammasome results in reduction diet-induced metabolic alterations. Male C57/BL6...
Non-Alcoholic Fatty Liver Disease (NAFLD) is a major form of chronic liver disease in the general population relation to its high prevalence among overweight/obese individuals and patients with diabetes type II or metabolic syndrome. NAFLD can progress steatohepatitis (NASH), fibrosis cirrhosis end-stage but mechanisms involved are still incompletely characterized. Within proposed mediate progression NAFLD, lipotoxicity believed play role. In present study we provide data suggesting that...