A5086190246- Pregnancy-related medical research
- Occupational Health and Performance
- Laser Applications in Dentistry and Medicine
- Pancreatic function and diabetes
- Paraoxonase enzyme and polymorphisms
- Nitric Oxide and Endothelin Effects
- Tissue Engineering and Regenerative Medicine
- Adipose Tissue and Metabolism
- Injury Epidemiology and Prevention
- Photodynamic Therapy Research Studies
- Asthma and respiratory diseases
- Heme Oxygenase-1 and Carbon Monoxide
- Mast cells and histamine
- Congenital heart defects research
- Dermatologic Treatments and Research
- Dental Implant Techniques and Outcomes
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Cardiac Ischemia and Reperfusion
- Nanoplatforms for cancer theranostics
- Oral microbiology and periodontitis research
- Body Contouring and Surgery
- Neuroendocrine Tumor Research Advances
- Spaceflight effects on biology
- Neuroblastoma Research and Treatments
University of Florence
2016-2025
Politecnico di Milano
2021
Florence (Netherlands)
2020
University of Chieti-Pescara
2020
Italian Association for Cancer Research
1992-2020
May Institute
2016
Centro di Riferimento Oncologico
2016
Don Carlo Gnocchi Foundation
2014
Institute of Forensic Science
2001-2012
University of Pisa
2008
Background— Serotonin (5-hydroxytryptamine [5-HT]), released by activated platelets during cardiac ischemia, is metabolized the mitochondrial enzyme monoamine oxidase A (MAO-A). Because hydrogen peroxide one of byproducts 5-HT degradation MAO-A, we investigated potential role reactive oxygen species generated MAOs in 5-HT-dependent cardiomyocyte death and post-ischemia-reperfusion damage. Methods Results— Treatment isolated adult rat cardiomyocytes with induced intracellular oxidative stress...
Proliferation of endothelial cells is regulated through the autocrine production growth factors and expression cognate surface receptors. In this study, we demonstrate that interleukin 1 (IL-1) an inhibitor in vitro vivo. IL-1 arrested growing, cultured G1 phase; inhibition proliferation was dose dependent occurred parallel with occupancy angiogenesis model, could inhibit fibroblast factor-induced vessel formation. The nature effect on demonstrated by observation cell-surface receptors...
Adipose tissue is a dynamic endocrine organ with central role in metabolism regulation.Functional differences adipose seem associated the regional distribution of fat depots, particular subcutaneous and visceral omental pads.Here, we report for first time isolation human adipose-derived adult stem cells from (V-ASCs S-ASCs, respectively) same subject.Immunophenotyping shows that plastic culturing selects homogeneous cell populations V-ASCs S-ASCs corresponding stromal vascular fractions...
Glomerular diseases account for 90% of end-stage kidney disease. Podocyte loss is a common determining factor the progression toward glomerulosclerosis. Mature podocytes cannot proliferate, but recent evidence suggests that they can be replaced by renal progenitors localized within Bowman's capsule. Here, we demonstrate Notch activation in human stimulates entry into S-phase cell cycle and division, whereas its downregulation required differentiation podocyte lineage. Indeed, persistent...
Recent evidence indicates that the adult heart contains sub-epicardial cardiogenic niches where cardiac stem cells and stromal supporting reside together. Such include a special population, previously identified as interstitial Cajal-like recently termed telocytes because of their long, slender processes (telopodes) embracing myocardial precursors. Specific cells, presumptively originated from epicardium, have been postulated to populate developing they are thought play role in its...
The hormone relaxin (RLX) is produced by the heart and has beneficial actions on cardiovascular system. We previously demonstrated that RLX stimulates mouse neonatal cardiomyocyte growth, suggesting its involvement in endogenous mechanisms of myocardial histogenesis regeneration. In present study, we extended experimentation evaluating effects primary cultures cardiac stromal cells. inhibited TGF-β1-induced fibroblast-myofibroblast transition, as judged ability to down-regulate α-smooth...
Among plasma proteins, fibrinogen represents a major target of oxidative modifications. In patients with post-acute myocardial infarction (6 months after the acute event), oxidation-induced carbonyls and function were estimated using in vitro ex vivo approaches. Fibrinogen structural features clot architecture also explored.In 39 28 age-, sex-, risk factor-matched controls, stress markers (in purified fractions), thrombin-catalyzed fibrin polymerization, plasmin-induced lysis estimated....
Abstract Acute kidney injury (AKI) is frequent, often fatal and, for lack of specific therapies, can leave survivors with chronic disease (CKD). We characterize the distribution tubular cells (TC) undergoing polyploidy along AKI by DNA content analysis and single cell RNA-sequencing. Furthermore, we study functional roles polyploidization using transgenic models drug interventions. identify YAP1-driven TC outside site as a rapid way to sustain residual function early during AKI. This...
Relaxin (RLX) is a multifunctional hormone which, besides its role in pregnancy and parturition, has also been shown to influence the cardiovascular system. In this study, we investigated effect of RLX on coronary flow rat guinea‐pig hearts, isolated perfused Langendorff apparatus. was either added perfusion fluid at concentration 5 × 10 −9 M for 20‐min perfusion, or given as bolus into aortic cannula concentrations , −8 dissolved 1 ml fluid. RLX, increased amount nitrite, stable end‐product...
Vitiligo is a skin disorder characterized by loss of functional melanocytes. Keratinocytes contribute to melanocyte homeostasis, and keratinocyte alteration may play role in dysfunction vitiligo. In particular, the release melanogenic mediators level functioning keratinocytes affect vitiligo epidermis. Keratinocyte-derived involved pigmentation, analysed situ hybridization, epidermal apoptosis, detected TUNEL assay electron microscopy, were evaluated lesional perilesional biopsies from 15...
Abstract Aim Comparing the efficacy of photoablative and photodynamic diode laser in adjunct to scaling ‐root planing ( SRP ) alone for treatment chronic periodontitis. Materials Methods Twenty‐six patients were studied. Maxillary left or right quadrants randomly assigned sham‐laser + . This consisted intra/extra‐pocket de‐epithelization with (λ = 810 nm), followed by single multiple treatments (once weekly, 4–10 applications, mean ± SD : 3.7 2.4) using 635 nm) 0.3% methylene blue as...
Objectives Tumour necrosis factor (TNF) receptor-associated periodic syndrome (TRAPS) is caused by TNFRSF1A mutations, known to induce intracellular retention of the TNFα receptor 1 (TNFR1) protein, defective TNFα-induced apoptosis, and production reactive oxygen species. As downregulation autophagy, main cellular pathway involved in insoluble aggregate elimination, has been observed increase inflammatory response, we investigated whether it plays a role TRAPS pathogenesis. Methods The...
Abstract Although recent preclinical and clinical studies have demonstrated that recombinant human relaxin (rh RLX ) may important therapeutic potential in acute heart failure chronic kidney diseases, the effects of rh administration against renal ischaemia/reperfusion (I/R) injury never been investigated. Using a rat model 1‐hr bilateral artery occlusion followed by 6‐hr reperfusion, we investigated (5 μg/Kg i.v.) given both at beginning after 3 hrs reperfusion. Acute attenuated functional...
Mesenchymal stromal cells (MSCs) are a promising cell candidate in tissue engineering and regenerative medicine. Their proliferative potential can be increased by low-level laser irradiation (LLLI), but the mechanisms involved remain to clarified. With aim of expanding therapeutic application LLLI MSC therapy, present study we investigated effects 635 nm diode on mouse proliferation underlying cellular molecular mechanisms, focusing attention Notch-1 signal activation membrane ion channel...
At present, mitotane (MTT) represents the first-line pharmacological approach for treatment of advanced adrenocortical carcinoma (ACC). Despite clear evidence that drug can reduce clinical signs steroid excess in secreting ACC, mechanism mediating possible toxic effect MTT on tumor cells still remains obscure. This study investigated intracellular events underlying by studying qualitative and quantitative alterations mitochondrial morphology functions human cancer cell lines, H295R SW13....
In animal models, the cardiotropic hormone relaxin has been shown to protect heart against ischemia and reperfusion-induced damage, acting by multiple mechanisms that primarily involve coronary vessels. This in vitro study evaluates whether also a direct protective action on cardiac muscle cells. H9c2 rat cardiomyoblasts primary mouse cardiomyocytes were subjected hypoxia reoxygenation. some experiments, was added preventatively before hypoxia; others, at To elucidate its of action, we...
Abstract —The peptide hormone relaxin (RLX) has been shown to elicit a powerful vasodilatory response in several target organs. This is mediated by the stimulation of intrinsic nitric oxide (NO) generation. The present study was designed clarify whether RLX directly promotes relaxation vascular smooth muscle cells through NO Vascular from bovine aortas were incubated with at concentrations ranging 1 nmol/L μmol/L. expression and activity synthase, production NO, intracellular levels cGMP Ca...
Relaxin was previously shown to cause coronary vasodilation and inhibit mast cell activation through a stimulation of endogenous nitric oxide production. This suggests that relaxin may have beneficial effects on ischemia-reperfusion-induced myocardial injury, which is triggered by endothelial damage impaired generation. In this study, we tested the effect isolated perfused guinea pig hearts subjected ischemia reperfusion. Ischemia induced ligature left anterior descending artery; removal...
Abstract Purpose: Activity of histidine decarboxylase, the key enzyme in synthesis histamine, has been shown to be increased several types human tumors. We attempted establish whether possible involvement decarboxylase and histamine colorectal carcinogenesis might mediated by activation cyclooxygenase-2 (COX-2) pathway. Experimental Design: Expression/activity content, prostaglandin E2 (PGE2) production were analyzed 33 cancer samples HT29, Caco-2, HCT116 colon cell lines. The effects...
Human bone marrow (BM) cells lacking T- and B-cell markers expressed RNA encoding interleukin (IL) 4 secreted detectable amounts of IL-4 in supernatants response to Fc epsilon or gamma receptor (Fc R R) cross-linking. In some experiments, IL-5 expression cross-linkage could also be detected. contrast, transcripts for, secretion of, IL-2, IL-6, interferon were never observed. The presence IL-3 the cultures was essential for production by non-B, non-T BM cross-linking enhanced Under same...