A5031456922- Ion channel regulation and function
- Muscle Physiology and Disorders
- Regulation of Appetite and Obesity
- Adipose Tissue and Metabolism
- Pregnancy-related medical research
- Exercise and Physiological Responses
- Adipokines, Inflammation, and Metabolic Diseases
- Cellular Mechanics and Interactions
- Laser Applications in Dentistry and Medicine
- Connexins and lens biology
- Erythrocyte Function and Pathophysiology
- Neuroscience and Neural Engineering
- Ion Channels and Receptors
- Sphingolipid Metabolism and Signaling
- Neuropeptides and Animal Physiology
- Heat shock proteins research
- Circadian rhythm and melatonin
- Muscle activation and electromyography studies
- Cellular transport and secretion
- Eating Disorders and Behaviors
- Neurobiology and Insect Physiology Research
- Hypothalamic control of reproductive hormones
- Ovarian function and disorders
- Apelin-related biomedical research
- GDF15 and Related Biomarkers
University of Florence
2016-2025
Translational Research Institute
2020
Azienda Ospedaliero-Universitaria Careggi
2020
Creative Research Enterprises (United States)
2020
Jackson and Tull (United States)
2020
Scientific Research Institute of Experimental and Clinical Medicine
2017
Institut de Myologie
2014
University of Padua
2009
Neuroscience Institute
2009
University of Bologna
2009
Regenerative medicine represents a critical clinical goal for patients with ESRD, but the identification of renal adult multipotent progenitor cells has remained elusive. It is demonstrated that in human kidneys, subset parietal epithelial (PEC) Bowman’s capsule exhibit coexpression stem cell markers CD24 and CD133 cell–specific transcription factors Oct-4 BmI-1, absence lineage-specific markers. This + PEC population, which could be purified from cultured capsulated glomeruli, revealed...
Adipose tissue is a dynamic endocrine organ with central role in metabolism regulation.Functional differences adipose seem associated the regional distribution of fat depots, particular subcutaneous and visceral omental pads.Here, we report for first time isolation human adipose-derived adult stem cells from (V-ASCs S-ASCs, respectively) same subject.Immunophenotyping shows that plastic culturing selects homogeneous cell populations V-ASCs S-ASCs corresponding stromal vascular fractions...
Metabolic pathologies mainly originate from adipose tissue (AT) dysfunctions. AT differences are associated with fat-depot anatomic distribution in subcutaneous (SAT) and visceral omental (VAT) pads. We address the question whether functional between two compartments may be present early stem cell (ASC) instead of being restricted to mature adipocytes. Using a specific human ASC model, we evaluated proliferation/differentiation abdominal SAT-(S-ASC) VAT-(V-ASC) paired biopsies parallel as...
Transient receptor potential canonical (TRPC) channels provide cation and Ca2+ entry pathways, which have important regulatory roles in many physio-pathological processes, including muscle dystrophy. However, the mechanisms of activation these remain poorly understood. Using siRNA, we first experimental evidence that TRPC channel 1 (TRPC1), besides acting as a store-operated channel, represents an essential component stretch-activated C2C12 skeletal myoblasts, assayed by whole-cell...
Mesenchymal stromal cells (MSCs) are a promising cell candidate in tissue engineering and regenerative medicine. Their proliferative potential can be increased by low-level laser irradiation (LLLI), but the mechanisms involved remain to clarified. With aim of expanding therapeutic application LLLI MSC therapy, present study we investigated effects 635 nm diode on mouse proliferation underlying cellular molecular mechanisms, focusing attention Notch-1 signal activation membrane ion channel...
Although sphingosine 1-phosphate (S1P) has been considered a potent regulator of skeletal muscle biology, acting as physiological anti-mitogenic and prodifferentiating agent, its downstream effectors are poorly known. In the present study, we provide experimental evidence for novel mechanism by which S1P regulates differentiation through regulation gap junctional protein connexin (Cx) 43. Indeed, treatment with greatly enhanced Cx43 expression intercellular communication during early phases...
Abstract In the present study, we investigated functional interaction between stress fibers (SFs) and stretch‐activated channels (SACs) its possible role in regulation of myoblast differentiation induced by switch to culture presence or absence sphingosine 1‐phosphate. It was found that there a clear temporal correlation SF formation SAC activation differentiating C2C12 myoblasts. Inhibition actin polymerization with specific Rho kinase inhibitor Y‐27632, significantly decreased sensitivity...
BACKGROUND AND OBJECTIVE Low‐level laser therapy (LLLT) or photobiomodulation is emerging as a promising new therapeutic option for fibrosis in different damaged and/or diseased organs. However, the anti‐fibrotic potential of this treatment needs to be elucidated and cellular molecular targets clarified. Here, we investigated effects low intensity 635 ± 5 nm diode irradiation on fibroblast–myofibroblast transition, key event onset fibrosis, some underlying mechanisms. MATERIALS METHODS...
Abstract Although telocytes (TCs) have been proposed to play a “nursing” role in resident satellite cell (SC)-mediated skeletal muscle regeneration, currently there is no evidence of TC-SC morpho-functional interaction following tissue injury. Hence, we explored the presence TCs and their relationship with SCs an ex vivo model eccentric contraction (EC)-induced damage. EC-injured muscles showed structural/ultrastructural alterations changes electrophysiological sarcolemnic properties. were...
Background: Growth Differentiation Factor 15 (GDF15) has been described as influencing skeletal physiology. Nevertheless, no systematic appraisal of the effect GDF15 on muscle tissues developed to present day. Objective: The aim work was review evidence topic. Methods: In this preregistered (https://osf.io/wa8xr), articles were retrieved from MEDLINE/PubMed, EMBASE, and WebOfScience. Inclusion criteria comprised studies humans or animal models, assessment peripheral local tissue...
Membrane-cytoskeleton interaction regulates transmembrane currents through stretch-activated channels (SACs); however, the mechanisms involved have not been tested in living cells. We combined atomic force microscopy, confocal immunofluorescence, and patch-clamp analysis to show that stress fibers (SFs) C2C12 myoblasts behave as cables that, tensed by myosin II motor, activate SACs modifying topography viscoelastic (Young's modulus hysteresis) electrical passive (membrane capacitance, C m )...
To define the time course and potential effects of electrical stimulation on permanently denervated muscle, we evaluated excitation-contraction coupling (ECC) rat leg muscles during progression to long-term denervation by ultrastructural analysis, specific binding dihydropyridine receptors, ryanodine receptor 1 (RYR-1), Ca channels extrusion pumps, gene transcription translation Ca-handling proteins, in vitro mechanical properties electrophysiological analyses sarcolemmal passive L-type...
Inflammatory pathways may impair central regulatory networks involving gonadotropin-releasing hormone (GnRH) neuron activity. Studies in humans are limited by the lack of human GnRH cell lines. To establish an vitro model neurons and analyze effects proinflammatory cytokines. The primary fetal hypothalamic cells (hfHypo) were isolated from 12-week-old fetuses. Responsiveness to kisspeptin, main neurons' physiological regulator, was evaluated for biological characterization. Tumor necrosis...
Sphingosine 1-phosphate (S1P) is a bioactive lipid that abundantly present in the serum and mediates multiple biological responses. With aim of extending our knowledge on role played by S1P regulation cytoskeletal reorganization, native as well C2C12 myoblasts stably transfected with green fluorescent protein (GFP)-tagged alpha- beta-actin constructs were stimulated (1 microM) observed under confocal multiphoton microscopes. The addition induced appearance actin stress fibres focal adhesions...
Skeletal muscle regeneration is severely compromised in the case of extended damage. The current challenge to find factors capable limiting degeneration and/or potentiating inherent regenerative program mediated by a specific type myoblastic cells, satellite cells. Recent studies from our groups and others have shown that bioactive lipid, sphingosine 1-phosphate (S1P), promotes myoblast differentiation exerts trophic action on denervated skeletal fibres. In present study, we examined effects...
The potential therapeutic applications of targeting brown adipose tissue open new clinical avenues in fighting against metabolic pathologies. However, due to the limited extension adult humans depots, which are dramatically reduced after birth, solid cell models study human adipogenesis and its regulatory factors pathophysiology urgently needed. Here, we generated a novel model stem cells, hfB-ASC, derived for first time from fetal interscapular fat depots. Besides characterization their...
The success of cellular cardiomyoplasty, a novel therapy for the repair postischemic myocardium, depends on anatomical integration engrafted cells with resident cardiomyocytes. Our aim was to investigate interaction between undifferentiated mouse skeletal myoblasts (C 2 C 12 cells) and adult rat ventricular cardiomyocytes in an vitro coculture model. Connexin43 (Cx43) expression, Lucifer yellow microinjection, Ca 2+ transient propagation, electrophysiological analysis demonstrated that were...
Thyroid hormones (TH) play an important role in the development of human brain, by regulating expression specific genes. Selective Alzheimer's disease indicator-1 ( seladin-1 ) is a recently discovered gene with neuroprotective properties, which has been found to be down-regulated brain regions affected disease. Seladin-1 anti-apoptotic properties mainly due inhibition activation caspase 3. The aim this study was determine whether may regarded as new mediator effects TH developing brain. In...