Photobiomodulation (PBM) has been used for bone regenerative purposes in different fields of medicine and dentistry, but contradictory results demand a skeptical look its potential benefits. This vitro study compared PBM potentiality by red (635 ± 5 nm) or near-infrared (NIR, 808 10 diode lasers violet-blue (405 light-emitting operating continuous wave with 0.4 J/cm² energy density, on human osteoblast mesenchymal stromal cell (hMSC) viability, proliferation, adhesion osteogenic...

Abstract Aim Comparing the efficacy of photoablative and photodynamic diode laser in adjunct to scaling ‐root planing ( SRP ) alone for treatment chronic periodontitis. Materials Methods Twenty‐six patients were studied. Maxillary left or right quadrants randomly assigned sham‐laser + . This consisted intra/extra‐pocket de‐epithelization with (λ = 810 nm), followed by single multiple treatments (once weekly, 4–10 applications, mean ± SD : 3.7 2.4) using 635 nm) 0.3% methylene blue as...

Mesenchymal stromal cells (MSCs) are a promising cell candidate in tissue engineering and regenerative medicine. Their proliferative potential can be increased by low-level laser irradiation (LLLI), but the mechanisms involved remain to clarified. With aim of expanding therapeutic application LLLI MSC therapy, present study we investigated effects 635 nm diode on mouse proliferation underlying cellular molecular mechanisms, focusing attention Notch-1 signal activation membrane ion channel...

BACKGROUND AND OBJECTIVE Low‐level laser therapy (LLLT) or photobiomodulation is emerging as a promising new therapeutic option for fibrosis in different damaged and/or diseased organs. However, the anti‐fibrotic potential of this treatment needs to be elucidated and cellular molecular targets clarified. Here, we investigated effects low intensity 635 ± 5 nm diode irradiation on fibroblast–myofibroblast transition, key event onset fibrosis, some underlying mechanisms. MATERIALS METHODS...

Abstract Background and Objectives Dental lasers represent a promising therapeutic tool in the treatment of periodontal peri‐implant diseases. However, their clinical application remains still limited. Here, we investigated potential biostimulatory effect low pulse energy neodymium:yttrium–aluminum–garnet (Nd:YAG) laser irradiation on different cells representative oral microenvironment elucidated underlying molecular mechanisms. Materials Methods Saos‐2 osteoblasts, H‐end endothelial cells,...

We aimed at quantifying the presence of periodontopathogens in gingival biopsies from periodontitis patients treated with different photoablative lasers (diode GaAs, Er:YAG, Nd:YAG, and CO(2) lasers) histologically analyzing their effects on gingiva.Substantial evidence indicates that intracellular location periodontal bacteria epithelium may contribute to chronic periodontitis.Sixteen adult subjects were subjected conventional scaling/root planing topical chlorhexidine, immediately...

Background: The use of lasers in periodontology is a matter debate, mainly because the lack consensual therapeutic protocols. In this randomized, split‐mouth trial, clinical efficacy two different photoablative dental lasers, erbium:yttrium‐aluminum‐garnet (Er:YAG) and diode, for treatment gingival hyperpigmentation compared. Methods: Twenty‐one patients requiring mild‐to‐severe were enrolled. Maxillary or mandibular left right quadrants randomly subjected to deepithelialization with either...

Background: The bacterial endotoxin lipopolysaccharide (LPS) represents a prime pathogenic factor of peri‐implantitis because its ability to adhere tenaciously dental titanium implants. Despite this, the current therapeutic approach this disease remains based mainly on decontamination, paying little attention neutralization bioactive products. purpose present study was evaluate whether irradiation with low‐energy neodymium‐doped:yttrium, aluminum, and garnet (Nd:YAG) laser, in addition...

In an attempt to verify the nature of bone resorption processes on surrounding radicular cysts, fragments cysts with adjacent tissue were studied by morphological, cytochemical and ultrastructural methods. Cyst fluid was analysed for its content cytokines osteolytic activity. The cyst wall exhibited several connective extensions which penetrated bone. Numerous multinucleated tartrate-resistant acid phosphatase (TRAP)-positive cells seen at tip intraosseous capsule in direct contact tissue....

The present in vitro study was designed to evaluate and compare the efficacy of: 1) different dental laser devices used photoablative (PA) mode, namely commercial CO(2), Er:YAG, Nd:YAG lasers a prototype diode (wavelength = 810 nm); 2) low-energy or light-emitting (LED) 630 nm), photodynamic (PD) mode together with photoactivated agent methylene blue; 3) chlorhexidine, as reference drug, reduce activation of macrophages by lipopolysaccharide (LPS), major pro-inflammatory gram-negative...

Bacterial contamination may seriously compromise successful implant osteointegration in the clinical practice of dental implantology. Several methods for eliminating bacteria from infected implants have been proposed, but none them shown to be an effective tool treatment peri-implantitis. In present study, we investigated efficacy pulsed neodymium:yttrium aluminum garnet laser irradiation (Nd:YAG) achieving bacterial ablation while preserving surface properties titanium implants. For this...

We previously showed that photoablative laser therapy followed by multiple photodynamic cycles (PAPD) after scaling/root planing (SRP) improved healing of periodontitis patients as compared with conventional SRP 1-year follow-up. This study reports the favorable results PAPD plus in chronic at a 4-year follow-up.Twenty-four were studied. Maxillary left or right quadrants randomly assigned to treatment sham-treatment and SRP. consisted of: (1) intra/extrapocket de-epithelization diode (λ 810...

The ultimate goal in implantology is to restore the whole tooth–gingival complex a fashion that cannot be distinguished from rest of natural dentition. This study assesses volumetric and clinical changes vestibular gingival soft tissues, crucial for satisfactory engraftment esthetic results, upon treatment with laser-aided pouch roll augmentation second-stage surgery dental implant uncovering. Twelve patients mild ridge deficiencies 16 edentulous sites, including distal elements, were...

Objective: The purpose of this study was to optimize gingival laser photoablation by thermographic and autofluorescent feedbacks. Background data: Photoablative treatment is commonly used for de-epithelization in patients with chronic periodontitis or hyperpigmentation. reduction collateral thermal damage periodontal tissues crucial optimal outcome. Methods: Nineteen periodontitis, seven whom showing hyperpigmentation, were subjected an 810 nm diode continuous (1 W, 66.67 J/cm2) pulsed wave...

Although photobiomodulation (PBM) has proven promising to treat wounds, the lack of univocal guidelines and a thorough understanding light–tissue interactions hampers its mainstream adoption for wound healing promotion. This study compared murine human fibroblast responses PBM by red (635 ± 5 nm), near-infrared (NIR, 808 1 violet-blue (405 nm) light (0.4 J/cm2 energy density, 13 mW/cm2 power density). Cell viability was not altered treatments. Light confocal laser scanning microscopy...

Background: Effects of conventional ultrasonic scaler versus an erbium:yttrium‐aluminum‐garnet (Er:YAG) laser on titanium surfaces contaminated with subgingival plaque from patients peri‐implantitis are evaluated in terms of: 1) and biocorroded oxide coating removal; 2) surface change induction; 3) residual biocompatibility toward osteoblasts. Methods: Subgingival plaque‐coated disks a moderately rough were fixed ethanol treated (metal tip) or Er:YAG (20.3 38.2 J/cm 2 ) non‐contact mode....