Letizia Canu
A5058896363- Adrenal and Paraganglionic Tumors
- Cancer, Hypoxia, and Metabolism
- Hormonal Regulation and Hypertension
- Pituitary Gland Disorders and Treatments
- Glioma Diagnosis and Treatment
- Neuroblastoma Research and Treatments
- Cancer, Lipids, and Metabolism
- Neuroendocrine Tumor Research Advances
- Mitochondrial Function and Pathology
- Metabolism, Diabetes, and Cancer
- Peroxisome Proliferator-Activated Receptors
- Enzyme function and inhibition
- Thyroid Cancer Diagnosis and Treatment
- Caveolin-1 and cellular processes
- Ocular Oncology and Treatments
- Molecular Biology Techniques and Applications
- Cellular Mechanics and Interactions
- Potassium and Related Disorders
- Ion Transport and Channel Regulation
- Gestational Diabetes Research and Management
- Diet and metabolism studies
- Extracellular vesicles in disease
- Venous Thromboembolism Diagnosis and Management
- Cardiovascular and exercise physiology
- Cancer Cells and Metastasis
University of Florence
2016-2025
Azienda Ospedaliero-Universitaria Careggi
2018-2025
Istituto Nazionale di Documentazione Innovazione e Ricerca Educativa
2021-2025
Tumour Institute of Tuscany
2023
Radboud University Nijmegen
2018-2021
Radboud University Medical Center
2018-2021
Université Paris Cité
2016
Universitätsklinikum Würzburg
2016
Hôpital Cochin
2016
Assistance Publique – Hôpitaux de Paris
2016
Cross-sectional imaging regularly results in incidental discovery of adrenal tumours, requiring exclusion adrenocortical carcinoma (ACC). However, differentiation is hampered by poor specificity characteristics. We aimed to validate a urine steroid metabolomics approach, using profiling as the diagnostic basis for ACC.
Malignant pheochromocytoma and paraganglioma (MPP) are characterized by prognostic heterogeneity. Our objective was to look for parameters of overall survival (OS) in MPP patients. Retrospective multicenter study a neck-thoraco-abdomino-pelvic CT or MRI at the time malignancy diagnosis European centers between 1998 2010. One hundred sixty-nine patients from 18 were included. Main characteristics with were: primary 53% patients; tumor- hormone-related symptoms 57% 58% cases; positive plasma...
Up to 7% of all adrenal incidentalomas (AIs) are pheochromocytomas (PCCs). In the evaluation AI, it is generally recommended that PCC be excluded by measurement plasma-free or 24-hour urinary fractionated metanephrines. However, recent studies suggest biochemical exclusion not performed for lesions with CT characteristics an adrenocortical adenoma (ACA).
Somatic mutations in PRKACA gene, encoding the catalytic subunit of protein kinase A (PKA), have been recently found a high proportion sporadic adenomas associated with Cushing's syndrome. The aim was to analyze mutation large cohort patients adrenocortical masses. Samples from nine European centers were included (Germany, n = 4; Italy, France, 1). drawn 149 nonsecreting (n 32 + 2 peritumoral), subclinical hypercortisolism 36), syndrome 64 androgen-producing tumors 4), carcinomas 5 and...
Adrenocortical carcinoma (ACC) has an aggressive but variable clinical course. Prognostic stratification based on the European Network for Study of Adrenal Tumours stage and Ki67 index is limited. We aimed to demonstrate prognostic role a points-based score (S-GRAS) in large cohort patients with ACC.This multicentre, retrospective study ACC who underwent adrenalectomy.The S-GRAS was calculated as sum following points: tumour (1-2 = 0; 3 1; 4 2), grade (Ki67 0-9% 10-19% ≥20% 2 points),...
BackgroundAdjuvant treatment with mitotane is commonly used after resection of adrenocortical carcinoma; however, remains controversial, particularly if risk recurrence not high. We aimed to assess the efficacy and safety adjuvant compared surveillance alone following complete tumour in patients carcinoma considered be at low intermediate recurrence.MethodsADIUVO was a multicentre, open-label, parallel, randomised, phase 3 trial done 23 centres across seven countries. Patients aged 18 years...
Evidence is limited regarding outcome of patients with ectopic Cushing's syndrome (ECS) due to neuroendocrine tumors (NETs).We assessed the prognostic factors affecting survival NETs and ECS.Retrospective analysis clinicopathological features, severity hormonal syndrome, treatments from a large cohort ECS collected 17 Italian centers.Our series included 110 patients, 58.2% female, mean (±s.d.) age at diagnosis 49.5 ± 15.9 years. The main sources ACTH were bronchial carcinoids (BC) (40.9%),...
Head and neck paragangliomas (HNPGLs) are neural crest-derived tumors. In comparison with located in the abdomen chest, which generally catecholamine secreting (sPGLs) sympathetic origin, HNPGLs are, fact, parasympathetic origin nonsecreting. Overall, 79 consecutive patients HNPGL were examined for mutations SDHA, SDHB, SDHC, SDHD, SDHAF2, VHL, MAX, TMEM127 genes by PCR/sequencing. According to a detailed family history (FH) clinical, laboratory (including metanephrines), instrumental...
Abstract Context Urine steroid metabolomics, combining mass spectrometry-based profiling and machine learning, has been described as a novel diagnostic tool for detection of adrenocortical carcinoma (ACC). Objective, Design, Setting This proof-of-concept study evaluated the performance urine metabolomics postoperative recurrence after microscopically complete (R0) resection ACC. Patients Methods 135 patients from 14 clinical centers provided samples, which were analyzed by gas...
At present, mitotane (MTT) represents the first-line pharmacological approach for treatment of advanced adrenocortical carcinoma (ACC). Despite clear evidence that drug can reduce clinical signs steroid excess in secreting ACC, mechanism mediating possible toxic effect MTT on tumor cells still remains obscure. This study investigated intracellular events underlying by studying qualitative and quantitative alterations mitochondrial morphology functions human cancer cell lines, H295R SW13....
Rationale: Microvascular obstruction (MVO) following percutaneous coronary intervention (PCI) is a common problem associated with adverse clinical outcomes.We are developing novel treatment, termed sonoreperfusion (SRP), to restore microvascular patency.This entails using ultrasound-targeted microbubble cavitation (UTMC) of intravenously administered gas-filled lipid microbubbles (MBs) dissolve obstructive microthrombi in the microvasculature.In our prior work, we used standard-sized MBs.In...
Abstract Context Because of the rarity adrenocortical cancer (ACC), only a few population-based studies are available, and they reported limited details in characterization patients their treatment. Objective To describe nationwide cohort presentation with ACC, treatment strategies, potential prognostic factors. Methods Retrospective analysis 512 diagnosed 12 referral centers Italy from January 1990 to June 2018. Results ACC as incidentalomas accounted for overall 38.1% cases, frequency that...
Background A substantial proportion of all pheochromocytomas is currently detected during the evaluation an adrenal incidentaloma. Recently, it has been suggested that biochemical testing to rule out pheochromocytoma unnecessary in case incidentaloma with unenhanced attenuation value ≤10 Hounsfield Units (HU) at computed tomography (CT). Objectives We aimed determine sensitivity 10 HU threshold exclude a pheochromocytoma. Methods Retrospective multicenter study systematic reassessment...
Adrenocortical carcinoma (ACC) is a rare heterogeneous malignancy with poor prognosis. Since radical surgery the only available treatment, more specific and effective drugs are urgently required. The anti-diabetic drug metformin has been associated decreased cancer prevalence mortality in several solid tumors, prompting its possible use for ACC treatment.This paper evaluates vitro vivo anti-cancer effects of using cell model H295R.Metformin treatment significantly reduces viability...
Mitotane is used as a post-operative adjuvant treatment for patients with adrenocortical carcinoma. Monitoring of plasma mitotane concentrations recommended, but we do not know what impact target have on patient outcome. To answer this question, retrospectively analyzed records in the Lysosafe Online® database (HRA Pharma, France) who were treated ≥6 months and had ≥3 measurements levels during follow-ups at 11 tertiary centers Italy from 2005 to 2017. We identified 110 median 46 (IQR,...
Abstract The crosstalk between the chromaffin and adrenocortical cells is essential for endocrine activity of adrenal glands. This interaction also likely important tumorigenesis progression cancer pheochromocytoma. We developed a unique in vitro 3D model whole gland called Adrenoid consisting carcinoma H295R pheochromocytoma MTT cell lines. Adrenoids showed round compact morphology with growth rate significantly higher compared to MTT-spheroids. Confocal analysis differential fluorescence...