A5081172698- Osteoarthritis Treatment and Mechanisms
- Cell Adhesion Molecules Research
- Ubiquitin and proteasome pathways
- Oral Health Pathology and Treatment
- Proteoglycans and glycosaminoglycans research
- Cancer-related Molecular Pathways
- Silk-based biomaterials and applications
- Salivary Gland Tumors Diagnosis and Treatment
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- Genetic factors in colorectal cancer
- Cancer-related molecular mechanisms research
- Head and Neck Cancer Studies
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Connective tissue disorders research
- HER2/EGFR in Cancer Research
- interferon and immune responses
- Extracellular vesicles in disease
- Colorectal Cancer Treatments and Studies
- Neurobiology and Insect Physiology Research
- ATP Synthase and ATPases Research
- Oral and Maxillofacial Pathology
- Protease and Inhibitor Mechanisms
- Cholangiocarcinoma and Gallbladder Cancer Studies
University of Genoa
1994-2025
Ospedale Policlinico San Martino
2015-2024
Creative Commons
2023
Istituto Giannina Gaslini
2021
Istituti di Ricovero e Cura a Carattere Scientifico
2012-2018
Istituto Nazionale di Fisica Nucleare, Sezione di Genova
2018
Alleanza Contro il Cancro
2004-2017
University of Turin
2013
Tecnologie Avanzate (Italy)
1994-2009
University of Naples Federico II
2008
This report deals with the quantitation of both mRNA and transcription activity type I collagen gene three cartilage-specific collagens (types II, IX, X) during in vitro differentiation chick chondrocytes. Differentiation was obtained by transferal to suspension culture dedifferentiated cells passaged for 3 wk as adherent cells. The mRNA, highly represented cells, rapidly decreased chondrocyte differentiation. On contrary, types II IX mRNAs sharply increased within first week culture, peaked...
In the developing chick embryo tibia type X collagen is synthesized by chondrocytes from regions of hypertrophy and not other (Capasso, O., G. Tajana, R. Cancedda, 1984, Mol. Cell. Biol. 4:1163-1168; Schmid, T. M., F. Linsenmayer, 1985, Dev. 107:375-381). To investigate further relationship between differentiation endochondral synthesis we have developed a novel culture system for 29-31-stage tibiae. At beginning these are small synthesize II collagen, but when grown on agarose-coated dishes...
Objectives Tumour necrosis factor (TNF) receptor-associated periodic syndrome (TRAPS) is caused by TNFRSF1A mutations, known to induce intracellular retention of the TNFα receptor 1 (TNFR1) protein, defective TNFα-induced apoptosis, and production reactive oxygen species. As downregulation autophagy, main cellular pathway involved in insoluble aggregate elimination, has been observed increase inflammatory response, we investigated whether it plays a role TRAPS pathogenesis. Methods The...
Differentiation of hypertrophic chondrocytes toward an osteoblast-like phenotype occurs in vitro when cells are transferred to anchorage-dependent culture conditions the presence ascorbic acid (Descalzi Cancedda, F., C. Gentili, P. Manduca, and R. Cancedda. 1992. J. Cell Biol. 117:427-435). This process is enhanced by retinoic addition medium. Here we compare growth undergoing this differentiation maintained suspension as such. The proliferation rate significantly higher adherent...
We developed a novel injectable carrageenan/fibrin/hyaluronic acid-based hydrogel with in situ gelling properties to be seeded chondrogenic cells and used for cartilage tissue engineering applications. first analysed the distribution within construct phenotype of human articular chondrocytes (HACs) cultured 3 weeks vitro. observed statistically significant increase cell number during 2 maintenance viability throughout culture, together deposition/formation cartilage-specific extracellular...
Natural killer cells are cytotoxic innate lymphoid that play an important role for early host defenses against infectious pathogens and surveillance tumor. In humans, NK may be divided in various subsets on the basis of relative CD56 expression low-affinity FcγRIIIA CD16. particular, two main cell represented by CD56bright/CD16-/dull CD56dull/CD16bright cells. Experimental evidences indicate CD56bright CD56dull represent different maturative stages developmental pathway. We identified...
ABSTRACT We have investigated the expression and localization of fibronectin, laminin, their receptors, we used an in vitro chick chondrocyte differentiation model to define a time hierarchy for appearance early chondrogenesis determine role cell condensation process. By serum fibronectin depletion/reconstitution, or GRGDSP peptide competition experiments, show that contributes initial cell-cell interactions occur during condensation. In later stages, downregulation both its α5β1 integrin...
Some new N ‐[6‐indazolyl]arylsulfonamides and ‐[alkoxy‐6‐indazolyl]arylsulfonamides were prepared by the reduction of 2‐alkyl‐6‐nitroindazoles with SnCl 2 in different alcohols, followed coupling corresponding amine arylsulfonyl chlorides pyridine. The newly synthesized compounds evaluated for their antiproliferative apoptotic activities against two human tumor cell lines: A2780 (ovarian carcinoma) A549 (lung adenocarcinoma). Preliminary vitro pharmacological studies revealed that...
Dufd1 (DUF729 domain containing 1) is related to Mtfr1 (mitochondrial fission regulator 1), a gene involved in the regulation of antioxidant activity mouse testis. The present study was undertaken better understand their role regulating mitochondrial architecture and function mouse. We show that expressed as 2 kb mRNA has more specific tissue pattern compared Mtfr1, with highest level expression testes, lower spleen, negligible levels other organs and/or tissues. In male gonad, increases...
ERBB2 is overexpressed in up to 20-30% of human breast cancers (BCs), and it associated with aggressive disease. Trastuzumab (Tz), a humanized monoclonal antibody, improves the prognosis ERBB2-amplified BCs. However, development resistance remains significant challenge. Carnosic acid (CA) diterpene found rosemary sage, endowed anticancer properties. In this vitro study, we have investigated whether Tz CA cooperative effects on cell survival overexpressing (ERBB2+) cells might restore...
Abstract Background This prospective observational study investigated the determinants of malignant transformation (MT) in localized oral leukoplakia (OL) and proliferative verrucous (PVL). Methods Demographic, clinical, histological, DNA ploidy status data were collected at enrolment. Survival analysis was performed (MT being event interest). Results One‐hundred thirty‐three patients with OL 20 PVL entered over 6 years (mean follow‐up 7.8 years). The presence OED, ploidy, clinical...
Abstract Background KRAS and BRAF mutations appear of relevance in the genesis progression several solid tumor types but co-occurrence interaction these have not yet been fully elucidated. Using a microsatellite stable (MSS) colorectal cancer (CRC) cell line (Colo741) having mutated WT , we also aimed to investigate KRAS-BRAF interaction. Gene expression profiles for control G 12 V D transfected cells were obtained after clone selection RT-PCR screening. Extensive qPCR was performed confirm...
Chromosomal instability and aneuploidy may represent biomarkers of oral exposure to damaging agents early signs clinical disease according the theory "oral field cancerization."The hypothesis was tested that DNA index (DI) values, obtained by high-resolution flow cytometry (DNA-FCM), potentially contribute cancer risk prediction. For this purpose, DI fields normal-appearing mucosa malignant disorders (OPMDs) in 165 consecutive patients for association with dysplasia and/or subsites tongue...
Metastatic uveal melanoma (MUM) is a highly aggressive, therapy-resistant disease. Driver mutations in Gα-proteins GNAQ and GNA11 activate MAP-kinase YAP/TAZ pathways of oncogenic signalling. MEK-inhibitors do not significantly block MUM progression, likely due to persisting Statins inhibit activation by blocking the mevalonate pathway, geranyl-geranylation, subcellular localisation Rho-GTPase. We investigated drugs that affect valproic acid, verteporfin statins, combination with...