A5065498690- Supramolecular Self-Assembly in Materials
- Bone Tissue Engineering Materials
- biodegradable polymer synthesis and properties
- Electrospun Nanofibers in Biomedical Applications
- RNA Interference and Gene Delivery
- Chemical Synthesis and Analysis
- Silk-based biomaterials and applications
- Proteoglycans and glycosaminoglycans research
- Hydrogels: synthesis, properties, applications
- Polymer Surface Interaction Studies
- Advanced biosensing and bioanalysis techniques
- Polydiacetylene-based materials and applications
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Peptides and Activities
- 3D Printing in Biomedical Research
- Glycosylation and Glycoproteins Research
- Osteoarthritis Treatment and Mechanisms
- Advanced Drug Delivery Systems
- Dental materials and restorations
- Calcium Carbonate Crystallization and Inhibition
- Plant Stress Responses and Tolerance
- Connective tissue disorders research
- Lipid Membrane Structure and Behavior
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
Universidade do Porto
2023-2024
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2023-2024
Hospital Israelita Albert Einstein
2024
University of Minho
2008-2023
University of Cambridge
2023
University of Washington
2023
Eindhoven University of Technology
2023
Queen Mary University of London
2014-2023
Northwestern University
2008-2023
Universidad de Londres
2017-2023
We report here the self-assembly of macroscopic sacs and membranes at interface between two aqueous solutions, one containing a megadalton polymer other, small self-assembling molecules bearing opposite charge. The resulting structures have highly ordered architecture in which nanofiber bundles align reorient by nearly 90 degrees as membrane grows. formation diffusion barrier upon contact liquids prevents their chaotic mixing. hypothesize that growth is then driven dynamic synergy osmotic...
Self‐assembly is a ubiquitous process in biology where it plays numerous important roles and underlies the formation of wide variety complex biological structures. Over past two decades, materials scientists have aspired to exploit nature's assembly principles create artificial materials, with hierarchical structures tailored properties, for fabrication functional devices. Toward this goal, both synthetic building blocks been subject extensive research self‐assembly. In fact, molecular...
Lung cancer is associated with very poor prognosis and considered one of the leading causes death worldwide. Here, highly potent selective biohybrid RNA interference (RNAi)‐peptide nanoparticles (NPs) are presented that can induce specific long‐lasting gene therapy in inflammatory tumor macrophages (TAMs), via an immune modulation milieu combined suppressor effects. The data here prove passive silencing be achieved cells using regular RNAi NPs. When M2 peptide–based targeted immunotherapy...
In nature, organisms control crystal nucleation and growth using organic interfaces as templates. Scientists, in the last decades, have tried to learn from nature how design biomimetic biomaterials inspired by hierarchical complex structure of bone other natural mineralised tissues or biomineralization process onto substrates promote osteoconductive properties implantable devices. The synthetic analogues, i.e., with a similar bone, would certainly constitute major breakthrough tissue...
In scaffold-based tissue engineering strategies, the successful regeneration of tissues from matrix-producing connective cells or anchorage-dependent (e.g. osteoblasts) relies on use a suitable scaffold. This study describes development and characterization SPCL (starch with epsilon-polycaprolactone, 30:70%) SPLA [starch poly(lactic acid), 30:70%] fibre-meshes, aimed at application in bone tissue-engineering strategies. Scaffolds based were prepared fibres obtained by melt-spinning...
Healthcare-associated infections resulting from bacterial attachment and biofilm formation on medical implants are posing significant challenges in particular with the emergence of resistance to antibiotics. Here, we report design, synthesis characterization self-assembled nanostructures, which integrate their surface antibacterial peptides. The WMR peptide, is a modification native sequence myxinidin, marine peptide isolated epidermal mucus hagfish, was used considering its enhanced...
The susceptibility of starch-based biomaterials to enzymatic degradation by amylolytic enzymes (glucoamylase and α-amylase) was investigated means incubating the materials with a buffer solution, containing at different concentrations combinations, 37 °C for 6 weeks. Two polymeric blends corn starch poly(ethylene-vinyl alcohol) copolymer poly(ε-caprolactone), designated SEVA-C SPCL, respectively, were studied. material characterized gravimetry measurements, tensile mechanical testing,...
Sunflower (Helianthus annuus L. cv. SH222) plants and calli were exposed to KCl stress for three weeks. Calli more tolerant than plants. decreased NO−3, Mn, Fe B levels in whole P, Ca Mg shoots. Ca, Mg, 100 mM‐stressed calli. Chlorophyll content, Fm (Fm−F0)/Fm ratio stressed leaves, while F0 increased only leaves severe (100 150 mM). Membrane permeability lipid peroxidation under all conditions mM calli, but remained unchanged 25 Salt also induced changes relating antioxidant enzymes: showed...
Bone morphogenetic proteins (BMPs) are cytokines with strong ability to promote new bone formation. Herein, we report the use of silk fibroin microparticles as carriers for delivery BMP-2, BMP-9 or BMP-14. BMP-containing were prepared by a mild methodology using dropwise addition ethanol, exhibiting mean diameters 2.7 +/- 0.3 microm. Encapsulation efficiencies varied between 67.9 6.1 % and 97.7 2.0 depending on type amount BMP loaded. Release kinetics showed that BMP-14 released in two...
Supramolecular chemistry offers an exciting opportunity to assemble materials with molecular precision. However, there remains unmet need turn self-assembly into functional and devices. Harnessing the inherent properties of both disordered proteins graphene oxide (GO), we report a protein-GO co-assembling system that through diffusion-reaction process disorder-to-order transitions generates hierarchically organized exhibit high stability access non-equilibrium on demand. We use experimental...
ABSTRACT The objective of this work was to evaluate the effects cadmium (Cd) exposure on sunflower (Helianthus annuus L.) plant growth, chlorophyll content, and fluorescence. Sunflower plants were exposed different concentrations Cd (0, 5, 50, 500 μ M) for 21 d. Growth parameters (organ length, fresh dry weights) determined results compared with two associated photosynthesis degradation: content fluorescence (an easy non-destructive method). Exposure significantly decreased growth by...
We developed a novel injectable carrageenan/fibrin/hyaluronic acid-based hydrogel with in situ gelling properties to be seeded chondrogenic cells and used for cartilage tissue engineering applications. first analysed the distribution within construct phenotype of human articular chondrocytes (HACs) cultured 3 weeks vitro. observed statistically significant increase cell number during 2 maintenance viability throughout culture, together deposition/formation cartilage-specific extracellular...
Atherosclerosis (AS) is a lipid-driven chronic inflammatory disease occurring at the arterial subendothelial space. Macrophages play critical role in initiation and development of AS. Herein, targeted codelivery anti-miR 155 anti-inflammatory baicalein exploited to polarize macrophages toward M2 phenotype, inhibit inflammation treat The system consists carrier-free strategy (drug-delivering-drug, DDD), fabricated by loading anti-miR155 on nanocrystals, named as nanorods (BNRs), followed...