A5038985451- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Additive Manufacturing and 3D Printing Technologies
- Bone Tissue Engineering Materials
- Tissue Engineering and Regenerative Medicine
- biodegradable polymer synthesis and properties
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nanofabrication and Lithography Techniques
- Cellular Mechanics and Interactions
- Neuroscience and Neural Engineering
- Graphene and Nanomaterials Applications
- Advanced Sensor and Energy Harvesting Materials
- Nerve injury and regeneration
- Silk-based biomaterials and applications
- Mesenchymal stem cell research
- Gut microbiota and health
- Polymer Surface Interaction Studies
- Innovations in Concrete and Construction Materials
- Liver physiology and pathology
- Polymer composites and self-healing
- Analytical chemistry methods development
- Advanced Materials and Mechanics
- Probiotics and Fermented Foods
- Anatomy and Medical Technology
- Gene Regulatory Network Analysis
Piaggio (Italy)
2016-2025
University of Pisa
2016-2025
Max Planck Institute for Extraterrestrial Physics
2023
Ingegneria dei Sistemi (Italy)
2014
Informa (Italy)
2014
Istituto di Fisiologia Clinica
2001-2008
University of Padua
2008
National Research Council
2008
National Academies of Sciences, Engineering, and Medicine
2007
Institute of Molecular and Cell Biology
2006
Four dimensional (4D) printing is an emerging technology with great capacity for fabricating complex, stimuli-responsive 3D structures, providing potential tissue and organ engineering applications. Although the 4D concept was first highlighted in 2013, extensive research has rapidly developed, along more-in-depth understanding assertions regarding definition of 4D. In this review, we begin by establishing criteria printing, followed summary state-of-the-art technological advances field....
Bioartificial blends of poly-(ε-caprolactone) (PCL) with a polysaccharide (starch, S; dextran, D; or gellan, G) (PCL/S, PCL/D, PCL/G 90.9/9.1 wt ratio) were prepared by solution−precipitation technique and widely characterized differential scanning calorimetry analysis (DSC), Fourier transform infrared-attenuated total reflectance spectroscopy (FTIR-ATR), optical microscopy (OM), wide-angle X-ray diffraction (WAXD), thermogravimetry (TGA). DSC showed that the reduced crystallinity PCL had...
A technique for controlled deposition of biomaterials and cells in specific complex architectures is described. It employs a highly accurate three-dimensional micropositioning system with pressure-controlled syringe to deposit biopolymer structures lateral resolution 5 microm. The pressure-activated microsyringe equipped fine-bore exit needle wide variety two- patterns on which be deposited can adhere. has been characterized terms parameters such as applied pressure, motor speed, line width...
Abstract The aim of this work was the preparation blends based on alginate and gelatin, with different weight ratio, to combine advantages these two natural polymers for application in cardiac tissue engineering. physicochemical characterization, performed by Fourier transform infrared spectroscopy, differential scanning calorimetry thermogravimetric analysis, revealed a good miscibility presence interactions among functional groups pure biopolymers. Concerning swelling degradation tests,...
Abstract Bone is a highly vascularized tissue, in which vascularization and mineralization are concurrent processes during skeletal development. Indeed, both components should be included any reliable adherent vitro model platform for the study of bone physiology pathogenesis disorders. To this end, we developed an model, using gelatin-nanohydroxyapatite (gel-nHA) three-dimensional (3D) bioprinted scaffold. First, seeded human mesenchymal stem cells (hMSCs) on scaffold, underwent osteogenic...
Bone tissue engineering has emerged as a promising strategy to overcome the limitations of current treatments for bone-related disorders, but trade-off between mechanical properties and bioactivity remains concern many polymeric materials. To address this need, novel blends poly-L-lactic acid (PLLA), polycaprolactone (PCL) poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) have been explored. Blend filaments comprising PLLA/PCL/PHBV at ratio 90/5/5 wt% prepared using twin-screw extrusion....
Abstract 3D bioprinting has developed tremendously in the last couple of years and enables fabrication simple, as well complex, tissue models. The international space agencies have recognized unique opportunities these technologies for manufacturing cell models basic research space, particular investigating effects microgravity cosmic radiation on different types human tissues. In addition, is capable producing clinically applicable grafts, its implementation therefore can support autonomous...
Abstract Mechanisms through which mature megakaryocytes (Mks) and their progenitors sense the bone marrow extracellular matrix to promote lineage differentiation in health disease are still partially understood. We found PIEZO1, a mechanosensitive cation channel, be expressed mouse human Mks. Human mutations PIEZO1 have been described associated with blood cell disorders. Yet, role for megakaryopoiesis proplatelet formation has never investigated. Here, we show that activation of increases...
Abstract Bioprinting technologies have been extensively studied in literature to fabricate three-dimensional constructs for tissue engineering applications. However, very few examples are currently available on clinical trials using bioprinted products, due a combination of technological challenges (i.e. difficulties replicating the native complexity, long printing times, limited choice printable biomaterials) and regulatory barriers no clear indication product classification current...
Elevated intraocular pressure may lead to retinal ganglion cell injury and consequent visual deficits. Chronic increase is a major risk factor for glaucoma, leading blinding disease, permanent deficits can also occur following acute increments due trauma, glaucoma or refractive surgery. How affects neurons not firmly established. Mechanical damage at the optic nerve head, reduced blood supply, inflammation cytotoxic factors have all been called into play. Reasoning that analysis of soon...
Abstract The replacement of injured cardiac contractile cells with stem cell-derived functionally efficient cardiomyocytes has been envisaged as the resolutive treatment for degenerative heart diseases. Nevertheless, many technical issues concerning optimal procedures to differentiate and engraft remain be answered before cell therapy could routinely used in clinical practice. So far, most studies have focused on evaluating differentiative potential different growth factors without...
Hydrogels are considered as excellent candidates for tissue substitutes by virtue of their high water content and biphasic nature. However, the fact that they soft, wet floppy renders them difficult to process use custom-designed scaffolds. To address this problem alginate hydrogels were modeled characterized measuring stress–strain creep behavior well viscosity a function sodium concentration, cross-linking time calcium ion concentration. The gels then microfabricated into scaffolds using...
A triphasic scaffold (TPS) for the regeneration of bone-ligament interface was fabricated combining a 3D fiber deposited polycaprolactone structure and polylactic co-glycolic acid electrospun. The presented gradient physical mechanical properties which elicited different biological responses from human mesenchymal stem cells. Biological test were performed on whole TPS scaffolds comprised each single part TPS, considered as controls. showed an increase metabolic activity with culturing time...
Developing green and nontoxic biomaterials, derived from renewable sources processable through 3D bioprinting technologies, is an emerging challenge of sustainable tissue engineering. Here, pectin citrus peels was cross-linked for the first time with (3-glycidyloxypropyl)trimethoxysilane (GPTMS) a one-pot procedure. Freeze-dried porous sponges, tunable properties in terms porosity, water uptake, compressive modulus, were obtained by controlling GPTMS content. Cell experiments showed that did...
Supramolecular chemistry offers an exciting opportunity to assemble materials with molecular precision. However, there remains unmet need turn self-assembly into functional and devices. Harnessing the inherent properties of both disordered proteins graphene oxide (GO), we report a protein-GO co-assembling system that through diffusion-reaction process disorder-to-order transitions generates hierarchically organized exhibit high stability access non-equilibrium on demand. We use experimental...
The aim of this work is the implementation a novel non-planar slicing algorithm, capable combining traditional planar layers with ones. geometry to be reconstructed will consist an inner volume made layers, and outer shell layers. Both are in turn constituted by region, shell, infill. guarantees enhancement aesthetic properties electro-mechanical behaviour printed objects, as well better adhesion when directly built onto complex geometry. algorithm was tested on three different...
This study aims to critically analyse the workflow of in situ bioprinting procedure, presenting a simulated neurosurgical case study, based on real traumatic event, for collecting quantitative data support this innovative approach. After event involving head, bone fragments may have be removed and replacement implant placed through highly demanding surgical procedure terms surgeon dexterity. A promising alternative current technique is use robotic arm deposit biomaterials directly onto...