A5069538965- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Electrospun Nanofibers in Biomedical Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Tissue Engineering and Regenerative Medicine
- Muscle Physiology and Disorders
- Bone Tissue Engineering Materials
- Pickering emulsions and particle stabilization
- Osteoarthritis Treatment and Mechanisms
- biodegradable polymer synthesis and properties
- Wound Healing and Treatments
- Extracellular vesicles in disease
- Tendon Structure and Treatment
- Silk-based biomaterials and applications
- RNA Interference and Gene Delivery
- Aerogels and thermal insulation
- Advanced biosensing and bioanalysis techniques
- Angiogenesis and VEGF in Cancer
- Cellular Mechanics and Interactions
- Periodontal Regeneration and Treatments
- Cell Adhesion Molecules Research
- Advanced Materials and Mechanics
- Neuroscience and Neural Engineering
- Cellular and Composite Structures
- Mesenchymal stem cell research
Institute of Physical Chemistry
2018-2025
Polish Academy of Sciences
2018-2025
Warsaw University of Technology
2016-2021
Sapienza University of Rome
1997-2019
Università Campus Bio-Medico
2016-2018
A novel bioink and a dispensing technique for 3D tissue-engineering applications are presented. The incorporates coaxial extrusion needle using low-viscosity cell-laden to produce highly defined biostructures. system is then coupled microfluidic device control the arrangement deposition, demonstrating versatility of bioprinting technique. This cell-responsive promotes cell migration alignment within each fiber organizing encapsulated cells. As service our authors readers, this journal...
Abstract The myocardium behaves like a sophisticated orchestra that expresses its true potential only if each member performs the correct task harmonically. Recapitulating complexity within engineered 3D functional constructs with tailored biological and mechanical properties, is one of current scientific priorities in field regenerative medicine tissue engineering. In this study, driven by necessity fabricating advanced model cardiac tissue, we present an innovative approach consisting...
We present a new strategy for the fabrication of artificial skeletal muscle tissue with functional morphologies based on an innovative 3D bioprinting approach. The methodology is microfluidic printing head coupled to co-axial needle extruder high-resolution hydrogel fibers laden precursor cells (C2C12). To promote myogenic differentiation, we formulated tailored bioink photocurable semi-synthetic biopolymer (PEG-Fibrinogen) encapsulating into constructs composed aligned fibers. After 3-5...
In this work we demonstrate how to print 3D biomimetic hydrogel scaffolds for cartilage tissue engineering with high cell density (>10(7) cells ml(-1)), viability (85 ÷ 90%) and printing resolution (≈100 μm) through a two coaxial-needles system. The were composed of modified biopolymers present in the extracellular matrix (ECM) cartilage, namely gelatin methacrylamide (GelMA), chondroitin sulfate amino ethyl methacrylate (CS-AEMA) hyaluronic acid (HAMA). polymers used prepare three...
Osteochondral (OC) tissue is a biphasic material comprised of articular cartilage integrated atop subchondral bone. Damage to this highly problematic, owing its intrinsic inability regenerate functional in response trauma or disease. Further, the function largely conferred by compartmentalized zonal microstructure and composition. Current clinical treatments fail new that recapitulates structure. Consequently, regenerated often lacks long-term stability. To address growing problem, we...
Next generation engineered tissue constructs with complex and ordered architectures aim to better mimic the native structures, largely due advances in three-dimensional (3D) bioprinting techniques. Extrusion has drawn tremendous attention its widespread availability, cost-effectiveness, simplicity, facile rapid processing. However, poor printing resolution low speed have limited fidelity clinical implementation. To circumvent downsides associated extrusion printing, microfluidic technologies...
Fiber-based approaches hold great promise for tendon tissue engineering enabling the possibility of manufacturing aligned hydrogel filaments that can guide collagen fiber orientation, thereby providing a biomimetic micro-environment cell attachment, migration, and proliferation. In this study, 3D system composed cell-laden, highly yarns is designed obtained via wet spinning in order to reproduce morphology structure fascicles. A bioink alginate gelatin methacryloyl (GelMA) optimized loaded...
In this study, we present an innovative strategy to reinforce 3D-printed hydrogel constructs for cartilage tissue engineering by formulating composite bioinks containing alginate and short sub-micron polylactide (PLA) fibers. We demonstrate that Young's modulus obtained pristine (6.9 ± 1.7 kPa) can be increased threefold (up 25.1 3.8 with the addition of PLA Furthermore, assess performance such materials in engineering, loaded human chondrocytes cultured vitro bioprinted up 14 days....
One promising strategy to reconstruct osteochondral defects relies on 3D bioprinted three-zonal structures comprised of hyaline cartilage, calcified and subchondral bone. So far, several studies have pursued the regeneration either cartilage or bone in vitro while-despite its key role region-only few them targeted layer. In this work, we present a biomimetic hydrogel scaffold containing β-tricalcium phosphate (TCP) for engineering through co-axial needle system implemented extrusion-based...
Abstract Tailoring the morphology of macroporous structures remains one biggest challenges in material synthesis. Herein, we present an innovative approach for fabrication custom materials which pore size varies throughout structure by up to order magnitude. We employed a valve‐based flow‐focusing junction (vFF) orifice can be adjusted real‐time (within tens milliseconds) generate foams with on‐line controlled bubble size. used fabricate layered and smoothly graded porous varying range...
In recent years, the main quest of science has been pioneering groundbreaking biomedical strategies needed for achieving a personalized medicine. Ribonucleic acids (RNAs) are outstanding bioactive macromolecules identified as pivotal actors in regulating wide range biochemical pathways. The ability to intimately control cell fate and tissue activities makes RNA-based drugs most fascinating family agents. However, widespread application RNA therapeutics humans is still challenging feat, due...
Abstract 3D bioprinting has developed tremendously in the last couple of years and enables fabrication simple, as well complex, tissue models. The international space agencies have recognized unique opportunities these technologies for manufacturing cell models basic research space, particular investigating effects microgravity cosmic radiation on different types human tissues. In addition, is capable producing clinically applicable grafts, its implementation therefore can support autonomous...
In this article, we have exploited a microfluidic foaming technique for the generation of highly monodisperse gas-in-liquid bubbles as templating system scaffolds characterized by an ordered and homogeneous porous texture. An aqueous poly(vinyl alcohol) (PVA) solution (containing surfactant) gas (argon) are injected simultaneously at constant flow rates in flow-focusing device (FFD), which thread breaks up to form bubbles. Immediately after its formation, foam is collected frozen liquid...
We demonstrate how to generate highly ordered porous matrices from dextran-methacrylate (DEX-MA) using microfluidics. use a flow focusing device inject an aqueous solution of DEX-MA and surfactant break the organic solvent (cyclohexane) into monodisperse droplets at high volume fraction (above 74% v/v) form internal phase emulsion (HIPE). collect crystalline HIPE structure freeze it by gelling. The resulting polyHIPEs are characterized interconnected morphology. size pores interconnects...
In this work, the influence of mechanical stiffness and geometrical confinement on 3D culture myoblast-laden gelatin methacryloyl (GelMA) photo-crosslinkable hydrogels was evaluated in terms vitro myogenesis. We formulated a set cell-laden GelMA with compressive modulus range 1 ÷ 17 kPa, obtained by varying concentration degree cross-linking. C2C12 myoblasts were chosen as cell model to investigate supportiveness different toward myotube formation up 2 weeks. Results showed that 3 kPa...
Three dimensional, periodic scaffolds of chitosan-coated alginate are fabricated in a layer-by-layer fashion by rapid prototyping. A novel dispensing system based on two coaxial needles delivers simultaneously and calcium chloride solutions permitting the direct deposition fibers according to any designed pattern. Coating fiber with chitosan subsequent cross-linking EDC genipin assured endurance scaffold culture environment for prolonged period time. The protocol adopted imparted...