A5070207840- Phosphodiesterase function and regulation
- Liver Disease Diagnosis and Treatment
- Diet, Metabolism, and Disease
- Pancreatic function and diabetes
- 3D Printing in Biomedical Research
- Cholinesterase and Neurodegenerative Diseases
- Drug Transport and Resistance Mechanisms
- Glycosylation and Glycoproteins Research
- Peroxisome Proliferator-Activated Receptors
- Liver physiology and pathology
- Metabolism and Genetic Disorders
- Protein Kinase Regulation and GTPase Signaling
- Biochemical effects in animals
- Amino Acid Enzymes and Metabolism
- Cell Adhesion Molecules Research
- Neuropeptides and Animal Physiology
- Cholesterol and Lipid Metabolism
- Renin-Angiotensin System Studies
- Clinical Nutrition and Gastroenterology
- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- Barrier Structure and Function Studies
- Retinoids in leukemia and cellular processes
- Proteins in Food Systems
- Receptor Mechanisms and Signaling
University of L'Aquila
2014-2024
Inserm
2009
University of California, San Francisco
1998-1999
San Francisco VA Medical Center
1998
Sapienza University of Rome
1988-1997
Istituto Nazionale di Fisica Nucleare, Sezione di Lecce
1994
Three dimensional, periodic scaffolds of chitosan-coated alginate are fabricated in a layer-by-layer fashion by rapid prototyping. A novel dispensing system based on two coaxial needles delivers simultaneously and calcium chloride solutions permitting the direct deposition fibers according to any designed pattern. Coating fiber with chitosan subsequent cross-linking EDC genipin assured endurance scaffold culture environment for prolonged period time. The protocol adopted imparted...
Highly open porous biodegradable scaffolds, based on gelatin A3, were fabricated with the aim of using them for tissue-engineering applications. The fabrication process is an emulsion-templating technique. In preparation scaffolds two different cross-linking procedures adopted: (I) radical polymerization methacrylate functionalities, previously introduced onto chains and (II) formation isopeptide bridges among promoted by enzyme microbial transglutaminase. method exerts a pronounced effect...
It is now well established that exposure of cells and tissues to nitric oxide leads the formation a dinitrosyl-iron complex bound intracellular proteins, but little known about how formed, identity physiological role this process. By using EPR spectroscopy enzyme activity measurements study mechanism in hepatocytes, we here identify as dinitrosyl-diglutathionyl-iron (DNDGIC) Alpha class glutathione S-transferases (GSTs) with extraordinary high affinity (KD = 10-10 m). This formed...
Gelatin is one of the most commonly used biopolymer for creating cellular scaffolds due to its innocuous nature. To create stable gelatin at physiological temperature (37 degrees C), chemical cross-linking a necessary step. In previous paper (Biomacromolecules 2006, 7, 3059-3068), was carried out by either radical polymerization methacrylated derivative (GMA) or through formation isopeptide bonds catalyzed transglutaminase. The method scaffold production based on emulsion templating in which...
High-fat and/or high-carbohydrate diets may predispose to several metabolic disturbances including liver fatty infiltration (hepatosteatosis) or be associated with necro-inflammation and fibrosis (steatohepatitis). Several studies have emphasized the hepatoprotective effect of some natural agents. In this study, we investigated potential therapeutic effects treatment emodin, an anthraquinone derivative anti-oxidant anti-cancer abilities, in rats developing diet-induced hepatosteatosis...
The development of blended gelatin and glycosaminoglycan (GAG) scaffolds can potentially be used in many soft tissue engineering applications since these mimic the structure biological function native extracellular matrix (ECM). In this study, we were able to obtain a gelatin–GAG scaffold by using concentrated emulsion templating technique known as high internal phase (HIPE), which prevailing volume organic is dispersed form discrete droplets inside an aqueous solution three biopolymers...
Abstract Injection of cell‐laden scaffolds in the form mesoscopic particles directly to site treatment is one most promising approaches tissue regeneration. Here, a novel and highly efficient method presented for preparation porous microbeads tailorable dimensions (in range ≈300–1500 mm) with uniform fully interconnected internal texture. The starts generation monodisperse oil‐in‐water emulsion inside flow‐focusing microfluidic device. This later broken‐up, use electric field, into double...
Origins of hyperlipidemia and cholestasis that occur during pregnancy were investigated by examining expression key elements related to plasma hepatic cholesterol metabolism pregnancy, lactation, post-lactation in the rat model. Among major findings were: activities 3-hydroxy-3-methylglutaryl coenzyme A reductase, acyl A:cholesterol acyltransferase, A:diacylglycerol 7α-hydroxylase, ester hydrolases, low density lipoprotein receptors, LRP, mdr2 significantly lower or similar non-pregnant...
Many whole cell-based assays in use today rely on flat, two-dimensional (2D) glass or plastic substrates that may not produce results characteristic of vivo conditions. In this study, a three-dimensional (3D) assay scaffold was fabricated using gas-in-foam templating technique. The made poly(vinyl alcohol), water-soluble synthetic polymer with excellent film-forming, emulsifying, and biocompatible properties widely used the biomedical field. preliminary rheological studies solution PVA...
ABSTRACT Type 4 cyclic nucleotide phosphodiesterases (PDE4) are major members of a superfamily enzymes (PDE) involved in modulation intracellular signaling mediated by cAMP. Broadly expressed most human tissues and present large amounts the liver, PDEs have last decade been key therapeutic targets for several inflammatory diseases. Recently, significant body work has underscored their involvement different kinds cancer, but with no attention paid to liver cancer. The study investigated...
Triclocarban (TCC), is an antimicrobial component in personal care products and it one of the emerging contaminants since has been detected various environmental matrices. Its presence human cord blood, breast milk, maternal urine raised issues about its possible impact on development increased concerns safety daily exposure. This study aims to provide additional information effects zebrafish early-life exposure TCC eye visual function. Zebrafish embryos were exposed two concentrations (5 50...
Cholesterol 7α-hydroxylase is a rate-limiting enzyme in bile acid synthesis, major pathway for cholesterol catabolism. It plays crucial role postnatal development and survival. In an adult liver, its activity messenger RNA (mRNA) are heterogeneously distributed with concentration the pericentral area. We defined how pattern of mRNA evolves during rat liver development, correlated this total levels, determined when heterogeneous expression established. was undetectable 18-day-old fetal livers...
Abstract Because of their peculiar physico-chemical properties, alginate beads have often been proposed as an alternative cell immobilization matrix for many biotechnological applications. For entrapped hepatocytes perfused in a bioreactor, demonstrated to promote viability and three-dimensional organization. In order optimise the hepatocyte culture, we investigated relationship between at high low content guluronic acid (G), relative reorganization when bioreactor. The primary structure...
Parkinson's disease is one of the most common neurologic disorder, affecting about 1-4% persons older than 60 years. Among proposed mechanisms PD generation, free radical damage believed to play a pivotal role in development and/or progression disease. Recently, PPARs, class transcription factors involved several pathways both physiological and pathological conditions, have been linked by us others neurodegeneration. Particularly, PPARγ its ligands indicated as potential therapeutic targets...
Non-alcoholic fatty liver disease (NAFLD) is nowadays considered as one of the most serious pathological conditions affecting liver. NAFLD supposed to be initiated by accumulation lipids in liver, which finally results an impaired hepatic insulin signalling. Many researchers have recently focused their attention on role played fructose a NAFLD-triggering agent, because increased diffusion fructose-sweetened food. However, epidemiological data do not permit evaluate per se, these foods are...