A5010525301- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Additive Manufacturing and 3D Printing Technologies
- Electrospun Nanofibers in Biomedical Applications
- Cystic Fibrosis Research Advances
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Silk-based biomaterials and applications
- Neuroscience and Neural Engineering
- Collagen: Extraction and Characterization
- Osteoarthritis Treatment and Mechanisms
- Gut microbiota and health
- Hydrogels: synthesis, properties, applications
- Dyeing and Modifying Textile Fibers
- Inflammatory mediators and NSAID effects
- Innovative Microfluidic and Catalytic Techniques Innovation
- Pharmacogenetics and Drug Metabolism
- Congenital Ear and Nasal Anomalies
- Genetic and Kidney Cyst Diseases
- Surgical Sutures and Adhesives
- Hernia repair and management
- History of Medical Practice
- Dental materials and restorations
- Intestinal and Peritoneal Adhesions
- Mathematical Biology Tumor Growth
University of Pisa
2013-2023
Piaggio (Italy)
2013-2023
Policlinico San Matteo Fondazione
2007
Abstract Bone is a highly vascularized tissue, in which vascularization and mineralization are concurrent processes during skeletal development. Indeed, both components should be included any reliable adherent vitro model platform for the study of bone physiology pathogenesis disorders. To this end, we developed an model, using gelatin-nanohydroxyapatite (gel-nHA) three-dimensional (3D) bioprinted scaffold. First, seeded human mesenchymal stem cells (hMSCs) on scaffold, underwent osteogenic...
Developing green and nontoxic biomaterials, derived from renewable sources processable through 3D bioprinting technologies, is an emerging challenge of sustainable tissue engineering. Here, pectin citrus peels was cross-linked for the first time with (3-glycidyloxypropyl)trimethoxysilane (GPTMS) a one-pot procedure. Freeze-dried porous sponges, tunable properties in terms porosity, water uptake, compressive modulus, were obtained by controlling GPTMS content. Cell experiments showed that did...
Clinical trials and animal studies on the gut microbiota are often limited by difficult access to gut, restricted possibility of in vivo monitoring, ethical issues. An easily accessible monitorable vitro model represents a valid tool for wider comprehension mechanisms which microbes interact with host each other. Herein, we present novel reliable system culturing human vitro. electrospun gelatin structure was biofabricated as scaffold microbial growth. The efficiency this supporting...
Gelatin is a natural biopolymer extensively used for tissue engineering applications due to its similarities the native extracellular matrix. However, rheological properties of gelatin formulations are not ideal extrusion-based bioprinting. In this work, we present an approach improve bioprinting performances by using pectin as rheology modifier and (3-glycidyloxypropyl)trimethoxysilane (GPTMS) gelatin–pectin crosslinking agent. The preparation initially optimized obtain homogenous gels....
Poultry feathers are among the most abundant and polluting keratin-rich waste biomasses. In this work, we developed a one-pot microwave-assisted process for eco-friendly keratin extraction from poultry followed by direct electrospinning (ES) of raw extract, without further purification, to obtain keratin-based bioplastics. This (MAE) was conducted in acetic acid 70% v/v. The effects time, solvent/feathers ratio, heating mode (MAE vs. conventional heating) on yield were investigated. highest...
Purpose The objective of the study was to compare intraocular pressure (IOP)-lowering efficacy and safety travoprost 0.004%/timolol 0.5% ophthalmic solution (Trav/Tim) latanoprost 0.005%/timolol (Lat/Tim), dosed once daily in morning, patients with open-angle glaucoma (OAG) or ocular hypertension (OH). Methods This a randomized, double-masked, multicenter, parallel group, active-controlled conducted at 41 sites. At eligibility visit were randomized (1:1) assigned masked medication if they...
The diagnosis of primary ciliary dyskinesia (PCD) can be challenging, and it may particularly difficult to distinguish disease from the secondary changes after infections.The purpose study was evaluate if nasal epithelial cells, obtained with brushing instead a biopsy, could used in culture system for PCD cases.Ciliary motion analysis (CMA) transmission electron microscopy (TEM) were performed on 59 subjects persistent or recurrent pneumonia. These investigations allowed 13 (22%) patients...
The aim of this study is the analysis and characterization a hydrolyzed keratin-based biomaterial its processing using electrospinning technology to develop in vitro tissue models. This biomaterial, extracted from poultry feathers, was mixed with type A porcine gelatin cross-linked γ-glycidyloxy-propyl-trimethoxy-silane (GPTMS) be casted initially form film characterized terms swelling, contact angle, mechanical properties surface charge density. After these chemical-physical...
Abstract Developing biomaterial formulations with specific biochemical characteristics and physical properties suitable for bioprinting of 3D scaffolds is a pivotal challenge in tissue engineering. Therefore, the design novel bioprintable continuously evolving research field. In this work, authors aim at expanding library inks by blending two natural biopolymers: pectin gelatin. Cytocompatible are obtained combining gelatin different ratios using (3‐glycidyloxypropyl)trimethoxysilane (GPTMS)...
Tendon and ligament injuries are relevant clinical problems in modern society, the current medical approaches do not guarantee complete recovery of physiological functionalities. Moreover, they present a non-negligible failure rate after surgery. Failures often occur at enthesis, which is area tendons ligaments insertion to bones. This highly anisotropic composed four distinct zones: tendon or ligament, non-mineralized fibrocartilage, mineralized bone. The organization these regions provides...
Decellularized bone matrix is receiving much attention as biological scaffolds and implantable biomaterials for tissue regeneration. Here, we evaluated the efficacy of a cell-free demineralized on mesenchymal stem cells (MSCs) survival differentiation in vitro. The seeding human umbilical cord-derived MSCs (hUC-SCs) decellularized matrices up to 14 days was exploited, assessing their capability scaffold colonization evaluating gene expression markers. Light Scanning Electron Microscopies...
For a deeper knowledge of phenomena at cell and tissue level, for understanding the role on bimolecular signalling development new drugs it is important to recreate in vitro environments that mimic physiological one. Spatial gradients soluble species guide cells' morphogenesis, they range three-dimensional (3D) environment. Gradients mechanical properties, which have 3D pattern, could lead migration differentiation. In this work, Concentration Gradient Maker able generate concentration was...
The aim of this study was set-up and test gelatin carbon nanotubes scaffolds. Gelatin-based (5%) genipin cross-linked (0.2%) scaffolds embedding single-walled (SWCNTs, 0.3, 0.5, 0.7, 0.9, 1.3% w/w) were prepared mechanically/electrically characterized. For biological evaluation, H9c2 cell line cultured for 10 days. Cytotoxicity, growth differentiation, immunohistochemistry, real-time PCR analysis performed. Myoblast cardiac differentiation obtained by serum reduction to 1% (C1% ) stimulation...
Diagnosis of primary ciliary dyskinesia (PCD) sometimes requires repeated nasal brushing to exclude secondary alterations. Our aim was evaluate whether the use a new method epithelial cell culture can speed PCD diagnosis in doubtful cases and identify which are most informative parameters by means multilayer artificial neural network (ANN). A cross-sectional study performed patients with suspected PCD. All underwent for motion analysis, ultrastructural assessment evaluation function after...
Genipin is a natural low-toxic cross-linker for molecules with primary amino groups, and its use collagen gelatin has shown great potential in tissue engineering applications. The fabrication of scaffolds well-organized micro macro topology using additive manufacturing systems requires an accurate control working parameters, such as reaction rate, gelling time, diffusion constant. A polymeric system 5% w/v PBS 2 mg/mL solutions 1:1 weight ratio was used template to perform measurements...
Today biomedical sciences are experiencing the importance of imaging biological parameters with luminescence methods. Studying 2D pH distribution those methods allows building knowledge about complex cellular processes. Immobilizing sensitive nanoparticles inside hydrogel matrixes, in order to guarantee a proper SNR, could easily make stable and biocompatible sensors. Inkjet printing is also well known as tool for images onto porous surfaces. Recently it has been used free-form fabrication...
Although the adhesion of bacteria on surfaces is a widely studied process, to date, most works focus single species microorganisms and are aimed at evaluating antimicrobial properties biomaterials. Here, we describe how complex microbial community, i.e., human gut microbiota, adheres surface form stable biofilms. Two electrospun structures made natural, gelatin, synthetic, polycaprolactone, polymers were used study their ability both promote microbiota support growth in vitro. Due different...
In the last decades drop-on-demand inkjet technology played an increasing role in industrial and medical applications. This is due to ability deposit a small amount of material precisely defined position. field Biofabrication, printers are used build 2D 3D scaffolds gels with biological molecules, including living cells. Several works, seminal papers on bioprinting, were carried out modified office printers. These have fixed structural characteristics operating size, especially print-head,...
Abstract The tissue integration and the formation of adhesions in repair abdominal wall defects are principally led to topology mechanical properties implanted prosthesis. In this study we analyzed influence meshes for repair, made polypropylene (PP), evaluating its ability prevent minimize adhesions, promote ingrowth. Two series vivo studies were performed. first, two types PP meshes, a lightweight macroporous mesh (LWM) heavyweight microporous (HWM) compared with determine optimal porosity...
In this work, we designed and realized a new phantom able to mimic the principal mechanical, rheological, physical cues of human eye that can be used as common benchmark validate surgical procedures, innovative vitrectomes, training system for surgeons. This phantom, in particular its synthetic humor vitreous, had aim reproducing diffusion properties natural evaluate pharmacokinetics drugs optimization their dose, limiting animal experiments. The was built layer-by-layer starting from sclera...
The biochemistry of a system made up three kinds cell is virtually impossible to work out without the use in silico models. Here, we deal with homeostatic balance phenomena from metabolic point view and present new computational model merging single-cell models, already available our research group: first reproduced behaviour hepatocyte, second one represented an endothelial cell, third described adipocyte. Multiple interconnections were created among these models order mimic main...