A5057499766- Osteoarthritis Treatment and Mechanisms
- Mesenchymal stem cell research
- Knee injuries and reconstruction techniques
- Tissue Engineering and Regenerative Medicine
- Periodontal Regeneration and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Tendon Structure and Treatment
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Cell Adhesion Molecules Research
- Orthopaedic implants and arthroplasty
- Silk-based biomaterials and applications
- Shoulder Injury and Treatment
- Total Knee Arthroplasty Outcomes
- Connective tissue disorders research
- Animal Genetics and Reproduction
- Developmental Biology and Gene Regulation
- TGF-β signaling in diseases
- Bone fractures and treatments
- Congenital heart defects research
- Wnt/β-catenin signaling in development and cancer
- Proteoglycans and glycosaminoglycans research
- Orthopedic Infections and Treatments
- Cancer-related gene regulation
- RNA Research and Splicing
Chinese University of Hong Kong
2018-2025
Hong Kong Science and Technology Parks Corporation
2022-2025
University of Pittsburgh
2015-2024
McGowan Institute for Regenerative Medicine
2013-2022
Prince of Wales Hospital
2022
Chinese University of Hong Kong, Shenzhen
2021
University of Hong Kong
2021
National Institutes of Health
2006-2020
Cornell University
2020
Harvard University
1978-2020
The architecture of an engineered tissue substitute plays important role in modulating growth. A novel poly(D,L-lactide-co-glycolide) (PLGA) structure with a unique produced by electrospinning process has been developed for tissue-engineering applications. Electrospinning is whereby ultra-fine fibers are formed high-voltage electrostatic field. electrospun structure, composed PLGA ranging from 500 to 800 nm diameter, features morphologic similarity the extracellular matrix (ECM) natural...
Abstract Human umbilical cord perivascular cells (HUCPVCs) have been shown to a high proliferative potential and the capacity differentiate into an osteogenic phenotype. HUCPVCs thus considered possible extra-embryonic mesenchymal stem cell (MSC) source for cell-based therapies. To assess this potential, we compared “gold standard” bone marrow stromal (BMSCs) with respect their proliferation, differentiation, transfection capacities. showed higher than BMSCs were capable of osteogenic,...
The ultimate goal of this study is to regenerate lost dental pulp and dentin via stem/progenitor cell-based approaches tissue engineering technologies. In study, we tested the possibility regenerating vascularized human in emptied root canal space producing new on existing dentinal walls using a cell-mediated approach with fragment an immunocompromised mouse model. Stem/progenitor cells from apical papilla stem were isolated, characterized, seeded onto synthetic scaffolds consisting...
Abstract Multipotential adult mesenchymal stem cells (MSCs) are able to differentiate along several known lineages, and lineage commitment is tightly regulated through specific cellular mediators interactions. Recent observations of a low/high bone‐mass phenotype in patients expressing loss‐/gain‐of‐function mutation LRP5, coreceptor the Wnt family signaling molecules, suggest importance bone formation, possibly involving MSCs. To analyze role osteogenesis, we have profiled expression WNTs...
Nanofibrous materials, by virtue of their morphological similarities to natural extracellular matrix, have been considered as candidate scaffolds for cell delivery in tissue-engineering applications. In this study, we evaluated a novel, three-dimensional, nanofibrous poly(epsilon-caprolactone) (PCL) scaffold composed electrospun nanofibers its ability maintain chondrocytes mature functional state. Fetal bovine (FBCs), maintained vitro between passages 2 6, were seeded onto three-dimensional...
Explant cultures of adult human trabecular bone fragments give rise to osteoblastic cells, that are known express osteoblast-related genes and mineralize extracellular matrix. These cells have also been shown undergo adipogenesis in vitro chondrogenesis vivo. Here we report the developmental potential (hOB) derived from explant collagenase-pretreated fragments. In addition osteogenic adipogenic differentiation, these capable chondrogenic differentiation a manner similar marrow-derived...
The multilineage differentiation potential of adult tissue-derived mesenchymal progenitor cells (MPCs), such as those from bone marrow and trabecular bone, makes them a useful model to investigate mechanisms regulating tissue development regeneration, cartilage. Treatment with transforming growth factor-beta (TGF-beta) superfamily members is key requirement for the in vitro chondrogenic MPCs. Intracellular signaling cascades, particularly involving mitogen-activated protein (MAP) kinases,...
Abstract Objective Mesenchymal stem cells (MSCs) are promising candidate for cartilage tissue engineering. Expression of hypertrophy markers (e.g., type X collagen) by MSCs undergoing chondrogenesis raises concern a engineering application MSCs, because would result in apoptosis and ossification. To analyze the biologic basis MSC hypertrophy, we examined response chondrifying to culture conditions known influence chondrocyte using an array hypertrophy‐associated markers. Methods Human pellet...
Transdifferentiation is a process whereby one cell type committed to and progressing along specific developmental lineage switches into another of different through genetic reprogramming. Even though this has been well studied established in amphibian systems, it unclear if mammalian cells possess the same potential. Recent vivo transplantation studies showed that adult mesenchymal stem (MSCs) were able differentiate mesoderm-derived types as with neuroectodermal endodermal characteristics,...
Generation of induced pluripotent stem (iPS) cells holds a great promise for regenerative medicine and other aspects clinical applications. Many types have been successfully reprogrammed into iPS in the mouse system; however, reprogramming human more difficult. To date, dermal fibroblasts are most accessible feasible cell source generation. Dental tissues derived from ectomesenchyme harbor mesenchymal-like stem/progenitor some treated as biomedical wastes, example, exfoliated primary teeth...