A5074340788- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Sensors
- Genetics, Aging, and Longevity in Model Organisms
- Biosensors and Analytical Detection
- biodegradable polymer synthesis and properties
- Nanoparticle-Based Drug Delivery
- Nematode management and characterization studies
- Oral microbiology and periodontitis research
- Electrospun Nanofibers in Biomedical Applications
- Nanoplatforms for cancer theranostics
- Parasite Biology and Host Interactions
- Bacterial biofilms and quorum sensing
- Photonic and Optical Devices
- Molecular Sensors and Ion Detection
- 3D Printing in Biomedical Research
- Parasites and Host Interactions
- Graphene and Nanomaterials Applications
- Photoacoustic and Ultrasonic Imaging
- Helminth infection and control
- SARS-CoV-2 detection and testing
- Advanced Polymer Synthesis and Characterization
- Ultrasound Imaging and Elastography
- Advanced Fluorescence Microscopy Techniques
- Nanoparticles: synthesis and applications
- Electrochemical sensors and biosensors
University of Nottingham
2015-2025
Advanced Materials and Technologies (Slovenia)
2025
Park University
2018
Extended dynamic range pH-sensitive ratiometric nanosensors, capable of accurately mapping the full physiological pH range, have been developed and used to characterize pharyngeal intestinal lumen Caenorhabditis elegans in real-time. Nanosensors, 40 nm diameter, were prepared by conjugating fluorophores, carboxyfluorescein (FAM) Oregon Green (OG) a 1:1 ratio, reference fluorophore, 5-(and-6)-carboxytetramethylrhodamine (TAMRA) an inert polyacrylamide matrix. Accurate measurements calculated...
Sustainably derived poly(glycerol adipate) (PGA) has been deemed to deliver all the desirable features expected in a polymeric scaffold for drug-delivery, including biodegradability, biocompatibility, self-assembly into nanoparticles (NPs) and functionalisable pendant group. Despite showing these advantages over commercial alkyl polyesters, PGA suffers from series of key drawbacks caused by poor amphiphilic balance. This leads weak drug-polymer interactions subsequent low drug-loading NPs,...
Abstract This report presents an optical fibre-based endo-microscopic imaging tool that simultaneously measures the topographic profile and 3D viscoelastic properties of biological specimens through phenomenon time-resolved Brillouin scattering. uses intrinsic viscoelasticity specimen as a contrast mechanism without fluorescent tags or photoacoustic mechanisms. We demonstrate 2 μm lateral resolution 320 nm axial for cells Caenorhabditis elegans larvae. has enabled first ever stiffness...
Ratiometric pH nanosensors with tuneable pKa were prepared by entrapping combinations of two pH-sensitive fluorophores (fluorescein isothiocyanate dextran (FITC-D) and Oregon Green®dextran (OG-D)) a reference fluorophore (5-(and-6)-carboxytetramethylrhodamine (TAMRA-D)), in biocompatible polymer matrix. Dual-fluorophore permit the measurement an extended dynamic range, from 4.0 to 7.5.
A custom designed microelectromechanical systems (MEMS) micro-hotplate, capable of operating at high temperatures (up to 700 °C), was used thermo-optically characterize fluorescent temperature-sensitive nanosensors. The nanosensors, 550 nm in diameter, are composed rhodamine B (RhB) fluorophore which conjugated an inert silica sol–gel matrix. Temperature-sensitive nanosensors were dispersed and dried across the surface MEMS mounted slide holder a fluorescence confocal microscope. Through...
Understanding the dynamic environmental microniches of biofilms will permit us to detect, manage and exploit these communities. The components architecture have been interrogated in depth; however, little is known about present. This primarily because absence tools with required measurement sensitivity resolution detect changes. We describe application ratiometric fluorescent pH-sensitive nanosensors, as a tool, observe physiological pH changes real time. Nanosensors comprised two...
Polymers are among the most studied materials as drug carriers, due to their tunable chemical structure and ability self-assemble give different types of nanostructures. In this study, chitosan (CS) nanoparticles (NPs) were investigated carriers for anticancer sodium usnate (NaU) treatment osteosarcoma (OS) is prevalent primary malignant bone sarcoma in pediatric adolescent patients. CS nano-assembling was induced by electrostatic interactions with anionic cross-linker tripolyphosphate, thus...
The nematode Caenorhabditis elegans is a key model organism in biological research due to its genetic similarity humans and utility studying complex processes. Traditional image analysis methods, such as those using ImageJ, are labour-intensive, which has led the integration of AI. This study introduces an AI framework with three machine learning models: WormGAN, Generative Adversarial Network for generating synthetic images enhance training data; WormDET, precise movement tracking; WormREG,...
The surface of an organism is a dynamic interface that continually adapts to its environment. In nematodes, the cuticle forms complex boundary protects against physicochemical pressures. However, precise molecular composition and function this remain largely unexplored. By utilizing 3D-OrbiSIMS, advanced surface-sensitive mass spectrometry method, we directly characterized outermost regions (∼50 nm) Caenorhabditis elegans Pristionchus pacificus improve understanding species-specific lipid...
Measurement of intracellular acidification is important for understanding fundamental biological pathways as well developing effective therapeutic strategies. Fluorescent pH nanosensors are an enabling technology real-time monitoring acidification. The physicochemical characteristics can be engineered to target specific cellular compartments and respond external stimuli. Therefore, represent a versatile approach probing inside cells. components comprise pH-sensitive fluorophore (signal...
Intracellular pH is a key parameter that influences many biochemical and metabolic pathways can also be used as an indirect marker to monitor intracellular processes. Herein, we utilise ratiometric fluorescent pH-sensitive nanosensors with extended dynamic range measure the of yeast (Saccharomyces cerevisiae) during glucose metabolism in real-time. Ratiometric consisting polyacrylamide nanoparticle matrix covalently linked two fluorophores, Oregon green (OG) 5(6)carboxyfluorescein (FAM),...
Newly synthesised Zn (<sc>ii</sc>) porphyrin nanoparticle conjugates were irradiated with visible light to generate controlled amounts of ROS in hMSCs advance the study oxidative stress and cellular communication.
The nematode Pristionchus pacificus has been established as a model for comparative studies using the well known Caenorhabditis elegans reference. Despite their relatedness, previous have revealed highly divergent development and number of morphological differences including lack pharyngal structure, grinder, used to physically lyse ingested bacteria in C. elegans. To complement current knowledge about developmental ecological with better understanding feeding modes, we sequenced intestinal...
Core–shell silica nanoparticles were shown to demonstrate quenching between a fluorescent core and surface black hole quencher layer, separated by size controllable shell.
Today biomedical sciences are experiencing the importance of imaging biological parameters with luminescence methods. Studying 2D pH distribution those methods allows building knowledge about complex cellular processes. Immobilizing sensitive nanoparticles inside hydrogel matrixes, in order to guarantee a proper SNR, could easily make stable and biocompatible sensors. Inkjet printing is also well known as tool for images onto porous surfaces. Recently it has been used free-form fabrication...
Nanosensors capable of simultaneously measuring dissolved oxygen concentrations from 0 to 100% saturation and pH over the full physiological range, 3.5 7.5, that advance methods towards understanding key biological gradients, were synthesised. A library water soluble oxygen-sensitive porphyrins, with three substituted charged functional groups a chemically flexible carboxylate group spectroscopically analysed assess their sensitivity changes in as free species solution suspension...
Despite the success of polyethylene glycol-based (PEGylated) polyesters in drug delivery and biomedical fields, concerns have arisen regarding PEG's immunogenicity limited biodegradability. In addition, inherent limitations, including chemical handles as well highly hydrophobic nature, can restrict their effectiveness physiological conditions polyester counterpart. To address these matters, an increasing amount research has been focused towards identifying alternatives to PEG. One promising...
Formulating protein therapeutics into nanoparticles (NPs) of poly(lactic-<italic>co</italic>-glycolic acid) (PLGA) provides key features such as protection against clearance, sustained release and less side effects by possible attachment targeting ligands.
Poly(lactic-co-glycolic acid) (PLGA) is a versatile synthetic copolymer that widely used in pharmaceutical applications. This because it well-tolerated the body, and copolymers of varying physicochemical properties are readily available via ring-opening polymerization. However, native PLGA polymers hard to track as drug delivery carriers when delivered subcellular spaces, due absence an easily accessible "handle" for fluorescent labeling. Here we show one-step, scalable, solvent-free, route...
Enteroviruses are ubiquitous mammalian pathogens that can produce mild to life-threatening disease. We developed a multimodal, rapid, accurate and economical point-of-care biosensor detect nucleic acid sequences conserved amongst 96% of all known enteroviruses. The harnesses the physicochemical properties gold nanoparticles oligonucleotides provide colourimetric, spectroscopic lateral flow-based identification an exclusive enteroviral sequence (23 bases), which was identified through in...