A5059528071- Protein purification and stability
- Polysaccharides Composition and Applications
- Glycosylation and Glycoproteins Research
- Neuroscience of respiration and sleep
- Polysaccharides and Plant Cell Walls
- Proteins in Food Systems
- Diabetes Management and Research
- Lignin and Wood Chemistry
- Crystallization and Solubility Studies
- Metabolism, Diabetes, and Cancer
- Food composition and properties
- Advanced Cellulose Research Studies
- Bacteriophages and microbial interactions
- Analytical Chemistry and Chromatography
- Pancreatic function and diabetes
- Advanced Drug Delivery Systems
- Postharvest Quality and Shelf Life Management
- Salivary Gland Disorders and Functions
- Cultural Heritage Materials Analysis
- Pressure Ulcer Prevention and Management
- Potato Plant Research
- Celiac Disease Research and Management
- Microfluidic and Capillary Electrophoresis Applications
- Lipid Membrane Structure and Behavior
- Sleep and Wakefulness Research
University of Nottingham
2014-2023
Sheffield Hallam University
2023
Queen's Medical Centre
2015-2022
Nottingham College
2013-2016
Loughborough University
2016
Georgetown University
1972-2007
McGill University
1972
Sedimentation equilibrium (analytical ultracentrifugation) is one of the most inherently suitable methods for determination average molecular weights and weight distributions polymers, because its absolute basis (no conformation assumptions) inherent fractionation ability (without need columns or membranes associated assumptions over inertness). With modern instrumentation it also possible to run up 21 samples simultaneously in a single run. Its application has been severely hampered...
Sustainably derived poly(glycerol adipate) (PGA) has been deemed to deliver all the desirable features expected in a polymeric scaffold for drug-delivery, including biodegradability, biocompatibility, self-assembly into nanoparticles (NPs) and functionalisable pendant group. Despite showing these advantages over commercial alkyl polyesters, PGA suffers from series of key drawbacks caused by poor amphiphilic balance. This leads weak drug-polymer interactions subsequent low drug-loading NPs,...
Abstract The development of new materials for the consolidation waterlogged archaeological wood from sustainable sources is an important area research, as most widely used consolidant today petroleum based. Ideally a will interact with existing structure, ensuring maximum compatibility. Lignin often major component remaining in wood, it less susceptible to degradation than holocellulose. Therefore, order maximise potential interaction cells, lignin-like oligomers have been synthesized...
The structure and function of clinical dosage insulin its analogues were assessed. This included 'native insulins' (human recombinant, bovine, porcine), 'fast-acting analogues' (aspart, glulisine, lispro) 'slow-acting (glargine, detemir, degludec). Analytical ultracentrifugation, both sedimentation velocity equilibrium experiments, employed to yield distributions molar mass coefficient all nine insulins. Size exclusion chromatography, coupled multi-angle light scattering, was also used...
The synaptic connection between the commissural portion of nucleus tractus solitarius (ComNTS) and dorsal motor vagus (DMV) was studied in rat brain stem slices, using patch-clamp technique. excitatory postsynaptic currents (EPSC) evoked by stimulation ComNTS were blocked kynurenic acid (1 mM) and, Mg2+-free solution, sensitive to both N-methyl-D-aspartic (NMDA) receptor blocker 3-[(RS)-2-carboxypiperazine-4-yl] -propyl-1-phosphonic (20 microM) non-NMDA...
MultiSig is a newly developed mode of analysis sedimentation equilibrium (SE) experiments in the analytical ultracentrifuge, having capability taking advantage remarkable precision (~0.1% signal) principal optical (fringe) system employed, thus supplanting existing methods through reducing 'noise' level certain important parameter estimates by up to orders magnitude. Long-known limitations SE method, arising from lack knowledge true fringe number optics and use unstable numerical algorithms...
Abstract Unusual protein-like, partially reversible associative behaviour has recently been observed in solutions of the water soluble carbohydrates known as 6-deoxy-6-(ω-aminoalkyl)aminocelluloses, which produce controllable self-assembling films for enzyme immobilisation and other biotechnological applications. Now, first time, we have found a fully self-association (tetramerisation) within this family polysaccharides. Remarkably these carbohydrate tetramers are then seen to associate...
Lanthanide binding site translation linearly along a coiled coil has large impact on stability, coordination chemistry, and MRI relaxivity.
VanA-type resistance to glycopeptide antibiotics in clinical enterococci is regulated by the VanS
Diabetes mellitus is associated with impaired wound healing. The topical use of insulin a promising therapy because it may favor all phases the healing process. This study aimed to investigate therapeutic outcomes gel in wounds hyperglycemic mice. After diabetes induction, 1-cm2 full-thickness was created on each animal's dorsum. lesions were treated daily for 14 days (insulin group) or vehicle without (vehicle group). Tissue samples extracted 4, 7, 10, and after creation lesion. analyzed...
Abstract The reversibility and strength of the previously established dimerization important glycopeptide antibiotic vancomycin in four different aqueous solvents (including a medically-used formulation) have been studied using short-column sedimentation equilibrium analytical ultracentrifuge model-independent SEDFIT-MSTAR analysis across range loading concentrations. change weight average molar mass M w with concentration was consistent monomer-dimer equilibrium. Overlap data sets point...
The heterogeneity and molecular weight of a chitosan low (molar mass) degree acetylation (0.1) for potential use as consolidant decayed archaeological wood were examined by sedimentation velocity equilibrium in the analytical ultracentrifuge before after depolymerisation. Sedimentation depolymerisation revealed uniform distribution coefficient with little concentration dependence. SEDFIT-MSTAR analysis average Mw (14.2 ± 1.2) kDa, polydispersity index ~ 1.2. Further using MULTISIG material...
A number of methods are available to researchers for estimating molecular weights (molar masses) and weight distributions starches − or those in solution, using an appropriate solvent/solubilisation protocol. We outline the assess their relative merits limitations. focus on size-exclusion chromatography field flow fractionation coupled multi-angle light scattering, viscometry, sedimentation velocity equilibrium analytical ultracentrifuge.
While a good mucoadhesive biopolymer must adhere to mucus membrane, it also have unloading ability. Here, we demonstrate that the pullulan is partially digested by human salivary α-amylase, thus acting as controlled release system, in which enzyme triggers an increased of flavour. Our oral processing simulations confirmed increase bioavailability aroma and salt compounds function degradation, although kinetics suggest rather slow process. One greatest challenges flavour science retain...
Abstract The 6-deoxy-6-aminocelluloses—or “aminocelluloses”—are a class of synthetic natural cellulose derivatives which are mostly aqueous soluble and have excellent film-forming properties. Recent studies connected these properties at the molecular level with protein-like self-associative behaviour for range aminocelluloses including 6-deoxy-6-(ω-aminoethyl) aminocellulose AEA-1 association being two-stage process—a reversible oligomerisation followed by further (semi-reversible)...
Abstract The natural glycopeptide antibiotic teicoplanin is used for the treatment of serious Gram-positive related bacterial infections and can be administered intravenously, intramuscularly, topically (ocular infections), or orally. It has also been considered targeting viral infection by SARS-CoV-2. hydrodynamic properties A2 ( M 1 = 1880 g/mol) were examined in phosphate chloride buffer (pH 6.8, I 0.10 M) using sedimentation velocity equilibrium analytical ultracentrifuge together with...