A5016163132- Metabolomics and Mass Spectrometry Studies
- Lipid metabolism and biosynthesis
- Immune Cell Function and Interaction
- Cancer, Hypoxia, and Metabolism
- Algal biology and biofuel production
- Advanced Proteomics Techniques and Applications
- Plant Micronutrient Interactions and Effects
- Phagocytosis and Immune Regulation
- Plant Stress Responses and Tolerance
- Sphingolipid Metabolism and Signaling
- Photosynthetic Processes and Mechanisms
- Mesenchymal stem cell research
- Metabolism, Diabetes, and Cancer
- Cellular transport and secretion
- Protein Kinase Regulation and GTPase Signaling
- Dysphagia Assessment and Management
- Mass Spectrometry Techniques and Applications
- Education and Public Policy
- Public Health in Brazil
- Diabetes and associated disorders
- Tryptophan and brain disorders
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Magnesium in Health and Disease
- Ion Transport and Channel Regulation
KU Leuven
2023-2025
VIB-KU Leuven Center for Cancer Biology
2023-2025
Institute of Protein Biochemistry
2021-2022
Centro Hospitalar Tondela-Viseu
2022
National Research Council
2021
Istituto di Chimica Biomolecolare
2021
University of Aveiro
2001-2019
Many individuals with cancer are resistant to immunotherapies. Here, we identify the gene encoding pyrimidine salvage pathway enzyme cytidine deaminase (CDA) among top upregulated metabolic genes in several immunotherapy-resistant tumors. We show that CDA cells contributes uridine diphosphate (UDP) pool. Extracellular UDP hijacks immunosuppressive tumor-associated macrophages (TAMs) through its receptor P2Y
Sunflower (Helianthus annuus L. cv. SH222) plants and calli were exposed to KCl stress for three weeks. Calli more tolerant than plants. decreased NO−3, Mn, Fe B levels in whole P, Ca Mg shoots. Ca, Mg, 100 mM‐stressed calli. Chlorophyll content, Fm (Fm−F0)/Fm ratio stressed leaves, while F0 increased only leaves severe (100 150 mM). Membrane permeability lipid peroxidation under all conditions mM calli, but remained unchanged 25 Salt also induced changes relating antioxidant enzymes: showed...
Hypoxia-inducible factor-1α (HIF1α) attenuates mitochondrial activity while promoting glycolysis. However, lower glycolysis is compromised in human clear cell renal carcinomas, which HIF1α acts as a tumor suppressor by inhibiting cell-autonomous proliferation. Here, we find that, unexpectedly, suppresses after the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) step, leading to reduced lactate secretion different types when cells encounter limited pyruvate supply such that typically found...
Obesity is a public health problem and risk factor for pathologies such type 2 diabetes mellitus, cardiovascular diseases, nonalcoholic fatty liver disease. Given these clinical implications, there growing interest to understand the pathophysiological mechanism of obesity. Changes in lipid metabolism have been associated with obesity obesity-related complications. However, changes profile obese children overlooked. In present work, we analyzed serum phospholipidome overweight by HILIC-MS/MS...
Mesenchymal stromal cells (MSCs) present anti-inflammatory properties and are being used with great success as treatment for inflammatory autoimmune diseases. In clinical applications MSCs subjected to a strong pro-inflammatory environment, essential their immunosuppressive action. Despite the wide use of these cells, how exert effect remains unclear. Several lipids known be involved in cell's signaling modulation cellular functions. The aim this paper is examine variation lipid profile...
Myocardium infarction is one of the most deathly cardiovascular diseases. It characterized by myocardium ischemia as a result nutrients depletion and hypoxia. The cell can respond to this injury autophagy or apoptosis, which determines evolution possible recovery infarction. Lipids play an important role in disease. However reports stating lipidome variations disease are scarce that lipids pathological condition not completely understood. aim work was identify changes lipid profile myoblast...
Abstract Sacoglossan sea slugs, also known as crawling leaves due to their photosynthetic activity, are highly selective feeders that incorporate chloroplasts from specific macroalgae. These “stolen” plastids - kleptoplasts kept functional inside animal cells and likely provide an alternative source of energy host. The mechanisms supporting the retention functionality remain unknown. A lipidomic mass spectrometry-based analysis was performed study kleptoplasty sacoglossan slug Elysia viridis...
Sunflower ( Helianthus annuus L. cv. SH222) plants and calli were exposed to KCl stress for three weeks. Calli more tolerant than plants. decreased NO −3 , Mn, Fe B levels in whole P, Ca Mg shoots. Ca, Mg, 100 mM‐stressed calli. Chlorophyll content, Fm − F0 )/ ratio stressed leaves, while increased only leaves severe (100 150 mM). Membrane permeability lipid peroxidation under all conditions mM calli, but remained unchanged 25 Salt also induced changes relating antioxidant enzymes: showed a...
<title>Abstract</title> In the brain, tryptophan byproducts are involved in biosynthesis of proteins, energy-rich molecules (e.g., NAD<sup>+</sup>), and neurotransmitters (serotonin melatonin). Impaired catabolism, seen aging, neurodegeneration psychiatric diseases affects mood, learning, sleep; however, reasons for those impairments remain unknown. Our results from cellular, <italic>drosophila melanogaster</italic>, mouse models indicate that SIRT6 regulates catabolism by balancing its...
Abstract Summary ADViSELipidomics is a novel Shiny app for preprocessing, analyzing and visualizing lipidomics data. It handles the outputs from LipidSearch LIQUID lipid identification quantification data Metabolomics Workbench. extracts information by parsing species (using LIPID MAPS classification) and, together with available on samples, performs several exploratory statistical analyses. When experiment includes internal standards, can normalize matrix, providing normalized concentration...
Glycerophosphoinositols (GPIs) are water-soluble bioactive phospholipid derivatives of increasing interest as intracellular and paracrine mediators eukaryotic cell functions. The most representative compound the family is glycerophosphoinositol (GroPIns), an ubiquitous component mammalian cells that participates in proliferation, survival response to stimuli. Levels activity this vary among types deciphering these functions requires accurate measurements vitro vivo models. conventional...
de 2019 até 2024, em língua inglesa e disponíveis forma completa gratuita.Foram excluídos artigos desalinhados com o tema do resumo incompletos.Resultados: Os estudos apontam que a abordagem multidisciplinar melhora significativamente os desfechos clínicos, uma redução no tempo atendimento internamento, além diminuir as taxas pacientes saem sem tratamento.Adicionalmente, foi visto equipes multidisciplinares promovem comunicação eficaz interações positivas entre membros, levam um melhor...
Diante da importância do tema e risco de complicações ainda mais graves, buscou-se reunir informações com o propósito responder ao seguinte problema pesquisa: Como a disfunção motora oral disfagia podem influenciar na qualidade vida no prognóstico dos recém nascidos?. Trata-se uma revisão sistemática literatura, finalidade sistematizar conhecimento sobre pela reunião principais dados disponíveis literatura. exposto, pode-se concluir escassez publicações acerca das disfagias neonatais,...
This clinical case reports a rare disease-Birt-Hogg-Dubé Syndrome-characterized by skin lesions and multiple lung cysts. Because of its rarity, BHDS is likely undiagnosed mistaken for primary spontaneous pneumothorax or emphysema. An early diagnosis important to set up screening renal cancer in patients affected relatives.
A fast and sensitive method that is based on Ultra High Performance Liquid Chromatography coupled with Resolution Mass Spectrometry (UHPLC-HRMS) for the measurement of Sulfavant A, a molecular adjuvant sulfolipid skeleton, described. The has been validated over linearity range 2.5–2000 ngmL−1 using deuterated derivative (d70-Sulfavant A) as internal standard. Chromatographic separation UHPLC Kinetex® 2.6 µm PS C18 column gradient methanol in 0.32 mM ammonium hydroxide solution buffered at pH...