A5061315439- Melanoma and MAPK Pathways
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Complex Network Analysis Techniques
- Opinion Dynamics and Social Influence
- Protein Degradation and Inhibitors
- Cancer Genomics and Diagnostics
- Cutaneous Melanoma Detection and Management
- RNA Research and Splicing
- CAR-T cell therapy research
- Privacy-Preserving Technologies in Data
- Cancer, Hypoxia, and Metabolism
- Immunotherapy and Immune Responses
- Game Theory and Applications
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Game Theory and Voting Systems
- Cellular transport and secretion
- Privacy, Security, and Data Protection
- Data Management and Algorithms
- Algorithms and Data Compression
- Cryptography and Data Security
- Immune cells in cancer
- Evolutionary Game Theory and Cooperation
VIB-KU Leuven Center for Cancer Biology
2018-2025
KU Leuven
2018-2024
University of Copenhagen
2024
Aarhus University
2020-2024
VIB-KU Leuven Center for Microbiology
2022
Vlaams Instituut voor Biotechnologie
2021
Aalborg University
2017-2018
Spanish National Cancer Research Centre
2014-2018
Centro de Investigación del Cáncer
2017-2018
University of Ioannina
2016
The Hippo signaling pathway and its two downstream effectors, the YAP TAZ transcriptional coactivators, are drivers of tumor growth in experimental models. Studying mouse models, we show that can also exert a tumor-suppressive function. We found normal hepatocytes surrounding liver tumors displayed activation deletion Yap Taz these peritumoral accelerated growth. Conversely, hyperactivation triggered regression primary melanoma-derived metastases. Furthermore, whereas cells growing wild-type...
Abstract Bidirectional signalling between the tumour and stroma shapes aggressiveness metastasis. ATF4 is a major effector of Integrated Stress Response, homeostatic mechanism that couples cell growth survival to bioenergetic demands. Using conditional knockout mice, we show global, or fibroblast-specific loss host ATF4, results in deficient vascularization pronounced delay syngeneic melanoma pancreatic tumours. Single-cell transcriptomics tumours grown Atf4 Δ/Δ mice uncovered reduction...
To better understand intrinsic resistance to immune checkpoint blockade (ICB), we established a comprehensive view of the cellular architecture treatment-naive melanoma ecosystem and studied its evolution under ICB. Using single-cell, spatial multi-omics, showed that tumor microenvironment promotes emergence complex transcriptomic landscape. Melanoma cells harboring mesenchymal-like (MES) state, population known confer targeted therapy, were significantly enriched in early on-treatment...
Deciphering the genomic regulatory code of enhancers is a key challenge in biology because this underlies cellular identity. A better understanding how work will improve interpretation noncoding genome variation and empower generation cell type–specific drivers for gene therapy. Here, we explore combination deep learning cross-species chromatin accessibility profiling to build explainable enhancer models. We apply strategy decipher melanoma, relevant case study owing presence distinct...
The ability to adapt environmental stress, including therapeutic insult, contributes tumor evolution and drug resistance. In suboptimal conditions, the integrated stress response (ISR) promotes survival by dampening cytosolic translation. We show that ISR-dependent also relies on a concomitant up-regulation of mitochondrial protein synthesis, vulnerability can be exploited using mitoribosome-targeting antibiotics. Accordingly, such agents sensitized MAPK inhibition, thus preventing...
Recent research studied the problem of publishing microdata without revealing sensitive information, leading to privacy-preserving paradigms k -anonymity and l -diversity. protects against identification an individual's record. -diversity, in addition, safeguards association individual with specific information. However, existing approaches suffer from at least one following drawbacks: (i) -diversification is solved by techniques developed for simpler -anonymization problem, causing...
Abstract Nuclear 3’-end-polyadenylation is essential for the transport, stability and translation of virtually all eukaryotic mRNAs. Poly(A) tail extension can also occur in cytoplasm, but transcripts involved are incompletely understood, particularly cancer. Here we identify a lineage-specific requirement cytoplasmic polyadenylation binding protein 4 (CPEB4) malignant melanoma. CPEB4 upregulated early melanoma progression, as defined by computational histological analyses. Melanoma cells...
Tumor cell plasticity, including transdifferentiation, is thought to be a key driver of therapy failure, tumor dormancy, and metastatic dissemination. Although melanoma cells have been shown adopt various phenotypic features in vitro, direct vivo evidence plasticity remains sparse. Here, we combine lineage tracing spontaneous mouse model melanoma, advanced imaging, single-cell RNA sequencing approaches search for pathophysiologically relevant cellular states. We identify intravascular niches...
Melanoma is a paradigm of aggressive tumors with complex and heterogeneous genetic background. Still, melanoma cells frequently retain developmental traits that trace back to lineage specification programs. In particular, lysosome-associated vesicular trafficking emerging as melanoma-enriched dependency. However, the contribution other lysosomal functions such autophagy progression unclear, particularly in context metastasis resistance targeted therapy. Here we mined broad spectrum cancers...
Today there is a strong interest in publishing set-valued data privacy-preserving manner. Such associate individuals to sets of values (e.g., preferences, shopping items, symptoms, query logs). In addition, an individual can be associated with sensitive label marital status, religious or political conviction). Anonymizing such implies ensuring that adversary should not able (1) identify individual's record, and (2) infer label, if exists. Existing research on this problem either perturbs the...
Abstract Primary resistance drastically limits the clinical success of immune checkpoint blockade (ICB) in melanoma. Resistance to ICB may also develop when tumours relapse after targeted therapy. To identify cancer cell-intrinsic mechanisms driving ICB, we generated single-cell RNA-sequencing (scRNA-seq) data from a prospective longitudinal cohort patients on therapy, including an early time point obtained only one cycle treatment. Comparing these with murine scRNA-seq datasets, established...
Increased pulmonary vascular resistance in hypertension (PH) is caused by vasoconstriction and obstruction of small arteries proliferating cells. In analogy to cancer, subsets cells may be derived from endothelial transitioning into a mesenchymal phenotype. To understand phenotypic shifts transpiring within PH, we injected rats with alkaloid monocrotaline induce PH measured lung tissue levels endothelial-specific protein critical differentiation marker (VE)-cadherin. VE-cadherin expression...
Syntactic data anonymization strives to (i) ensure that an adversary cannot identify individual's record from published attributes with high probability, and (ii) provide utility. These mutually conflicting goals can be expressed as optimization problem privacy the constraint utility objective function. Conventional research using k-anonymity model has resorted publishing in homogeneous generalized groups. A recently proposed alternative does not create such cliques; instead, it recasts...