Joke Van Elsen
A5001195292- Liver Disease Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Metabolism, Diabetes, and Cancer
- Lipid metabolism and biosynthesis
- T-cell and B-cell Immunology
- Adipose Tissue and Metabolism
- RNA modifications and cancer
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Nerve injury and regeneration
- Glycosylation and Glycoproteins Research
- PI3K/AKT/mTOR signaling in cancer
- Polyamine Metabolism and Applications
- Parkinson's Disease Mechanisms and Treatments
- Protein Degradation and Inhibitors
- CAR-T cell therapy research
VIB-KU Leuven Center for Cancer Biology
2020-2023
KU Leuven
2020-2022
Janssen (Belgium)
2021
Abstract Hepatic fat accumulation is associated with diabetes and hepatocellular carcinoma (HCC). Here, we characterize the metabolic response that high-fat availability elicits in livers before disease development. After a short term on diet (HFD), otherwise healthy mice showed elevated hepatic glucose uptake increased contribution to serine pyruvate carboxylase activity compared control (CD) mice. This phenotype occurred independently from transcriptional or proteomic programming, which...
Cancer cells display an increased plasticity in their lipid metabolism, which includes the conversion of palmitate to sapienate via enzyme fatty acid desaturase 2 (FADS2). We find that FADS2 expression correlates with mammalian target rapamycin (mTOR) signaling and sterol regulatory element-binding protein 1 (SREBP-1) activity across multiple cancer types is prognostic some types. Accordingly, activating mTOR by deleting tuberous sclerosis complex (Tsc2) or overexpression SREBP-1/2...
T cells dynamically rewire their metabolism during an immune response. We applied single-cell RNA sequencing to CD8+ activated and differentiated in vitro physiological medium resolve these metabolic dynamics. identify a differential time-dependent reliance of activating on the synthesis versus uptake various non-essential amino acids, which we corroborate with functional assays. also genes that potentially dictate outcome cell differentiation, by ranking them based expression Among them,...
Abstract Background Accumulation of misfolded a‐synuclein protein in intracellular inclusion bodies dopaminergic neurons underlies the pathogenesis synucleinopathies, which include Parkinson’s Disease (PD), Dementia with Lewy Bodies (DLB) and Multiple System Atrophy (MSA). Therefore, clearance α‐synuclein from could principle offer a an approach for modifying currently remain untreatable. Method In this study, we employ Affinity‐directed PROtein Missile (AdPROM) system consisting substrate...
Abstract Cancer metastasis requires the transient activation of cellular programs enabling dissemination and seeding in distant organs. Genetic, transcriptional translational intra-tumor heterogeneity contributes to this dynamic process. Beyond this, metabolic has also been observed, yet its role for cancer progression remains largely elusive. Here, we discovered that phosphoglycerate dehydrogenase (PHGDH) protein expression drives breast cell formation. Specifically, observed heterogeneous...
ABSTRACT Cytotoxic T cells dynamically rewire their metabolism during the course of an immune response. While T-cell has been extensively studied at phenotypic endpoints activation and differentiation, underlying dynamics remain largely elusive. Here, we leverage on single-cell RNA-sequencing (scRNA-seq) measurements in vitro activated differentiated CD8 + cultured physiological media to resolve these metabolic dynamics. We find that our scRNA-seq analysis identifies most changes previously...
<div>Abstract<p>Hepatic fat accumulation is associated with diabetes and hepatocellular carcinoma (HCC). Here, we characterize the metabolic response that high-fat availability elicits in livers before disease development. After a short term on diet (HFD), otherwise healthy mice showed elevated hepatic glucose uptake increased contribution to serine pyruvate carboxylase activity compared control (CD) mice. This phenotype occurred independently from transcriptional or proteomic...
<p>This document contains supplemental figures 1-7 and detailed methods</p>
<p>Supplemental tables 1-10.</p>
<p>Supplemental tables 1-10.</p>
<p>This document contains supplemental figures 1-7 and detailed methods</p>
<div>Abstract<p>Hepatic fat accumulation is associated with diabetes and hepatocellular carcinoma (HCC). Here, we characterize the metabolic response that high-fat availability elicits in livers before disease development. After a short term on diet (HFD), otherwise healthy mice showed elevated hepatic glucose uptake increased contribution to serine pyruvate carboxylase activity compared control (CD) mice. This phenotype occurred independently from transcriptional or proteomic...
Abstract Cancer cells outgrowing in distant organs of metastasis rewire their metabolism to fuel on the available nutrients. While this is often considered an adaptive pressure limiting formation, some nutrients at metastatic site naturally or through changes organ physiology may inherently promote growth. We find that lung, a frequent metastasis, lipid-rich environment. Moreover, we observe pathological conditions such as pre-metastatic niche formation and obesity further increase...