Sigrid Hatse
A5088923965- HIV Research and Treatment
- Chemokine receptors and signaling
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- HIV/AIDS drug development and treatment
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Frailty in Older Adults
- Cancer Genomics and Diagnostics
- Breast Cancer Treatment Studies
- Carbohydrate Chemistry and Synthesis
- Monoclonal and Polyclonal Antibodies Research
- Immune cells in cancer
- Cancer Risks and Factors
- Nutrition and Health in Aging
- Telomeres, Telomerase, and Senescence
- interferon and immune responses
- Gestational Trophoblastic Disease Studies
- Cytokine Signaling Pathways and Interactions
- Cancer Treatment and Pharmacology
- Genetic factors in colorectal cancer
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- Biochemical and Molecular Research
- Advanced Breast Cancer Therapies
KU Leuven
2016-2025
Universitair Ziekenhuis Leuven
2011-2024
Ospedale Policlinico San Martino
2019
University of Genoa
2019
Université Libre de Bruxelles
2017
Institut Jules Bordet
2017
VIB-KU Leuven Center for Microbiology
2017
Statistics Belgium
2017
Cancer Institute (WIA)
2012-2015
Helsinki University Hospital
2012
Two prominent members of the ATP-binding cassette superfamily transmembrane proteins, multidrug resistance 1 (MDR1) P-glycoprotein and protein (MRP1), can mediate cellular extrusion xenobiotics (anticancer) drugs from normal tumor cells. The MRP subfamily consists at least six members, here we report functional characterization human MRP5. We found against thiopurine anticancer drugs, 6-mercaptopurine (6-MP) thioguanine, anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine (PMEA) in...
This study was undertaken to demonstrate the unique specificity of chemokine receptor CXCR4 antagonist AMD3100. Calcium flux assays with selected chemokine/cell combinations, affording distinct specificities, revealed no interaction AMD3100 any receptors CXCR1 through CXCR3, or CCR1 CCR9. In contrast, potently inhibited CXCR4-mediated calcium signaling and chemotaxis in a concentration-dependent manner different cell types. Also, stromal cell-derived factor (SDF)-1-induced endocytosis CXCR4,...
Autoimmune collagen-induced arthritis (CIA) in IFN-gammaR-deficient DBA/1 mice was shown to be reduced severity by treatment with the bicyclam derivative AMD3100, a specific antagonist of interaction between chemokine stromal cell-derived factor-1 (SDF-1) and its receptor CXCR4. The beneficial effect CXCR4 demonstrable when initiated time immunization appearance first symptoms. Treatment also delayed-type hypersensitivity response autoantigen, collagen type II. These observations are...
Importance Young women with breast cancer who have germline pathogenic variants in BRCA1 or BRCA2 face unique challenges regarding fertility. Previous studies demonstrating the feasibility and safety of pregnancy survivors included limited data BRCA carriers. Objective To investigate cumulative incidence disease-free survival young are Design, Setting, Participants International, multicenter, hospital-based, retrospective cohort study conducted at 78 participating centers worldwide. The...
ABSTRACT The plant lectins derived from Galanthus nivalis (Snowdrop) (GNA) and Hippeastrum hybrid (Amaryllis) (HHA) selectively inhibited a wide variety of human immunodeficiency virus type 1 (HIV-1) HIV-2 strains clinical (CXCR4- CCR5-using) isolates in different cell types. They also efficiently infection T lymphocytes by mutant strains. GNA HHA markedly prevented syncytium formation between persistently infected HUT-78/HIV cells uninfected lymphocytes. did not measurably affect the...
Mannose-binding proteins derived from several plants (<i>i.e. Hippeastrum</i> hybrid and <i>Galanthus nivalis</i> agglutinin) or prokaryotes (<i>i.e.</i> cyanovirin-N) inhibit human immunodeficiency virus (HIV) replication select for drug-resistant viruses that show profound deletion of <i>N</i>-glycosylation sites in the GP120 envelope (Balzarini, J., Van Laethem, K., Hatse, S., Vermeire, De Clercq, E., Peumans, W., Damme, Vandamme, A.-M., Bolmstedt, A., Schols, D. (2004) <i>J. Virol.</i>...
AMD3465 is a novel, nonpeptide CXCR4 antagonist and potent inhibitor of HIV cell entry in that one the four-nitrogen cyclam rings symmetrical, prototype bicyclam AMD3100 has been replaced by two-nitrogen N-pyridinylmethylene moiety. This substitution induced an 8-fold higher affinity as determined against (125)I-12G5 monoclonal antibody binding, 22-fold potency inhibition CXCL12-induced signaling through phosphatidylinositol accumulation. Mutational mapping series analogs this library 23...
The redesign of azamacrocyclic CXCR4 chemokine receptor antagonists resulted in the discovery novel, small molecule, orally bioavailable compounds that retained T-tropic (CXCR4 using, X4) anti-HIV-1 activity. A structure−activity relationship (SAR) was determined on basis inhibition replication X4 HIV-1 NL4.3 MT-4 cells. As a result lead optimization, we identified (S)-N′-((1H-benzo[d]imidazol-2-yl)methyl)-N′-(5,6,7,8-tetrahydroquinolin-8-yl)butane-1,4-diamine (AMD070) 2 as potent and...
We correlated serum 25-hydroxyvitamin D 3 (25OHD) levels with tumor characteristics and clinical disease outcome in breast cancer patients assessed the impact of genetic determinants vitamin insufficiency. collected from 1800 early at diagnosis, measured 25OHD by radioimmunoassay (RIA), determined variants D-related genes Sequenom. Multivariable regression models were used to correlate characteristics. Cox proportional hazard assess overall survival (OS), disease-specific (DSS), disease-free...
Recent genome-wide association studies identified 11 single nucleotide polymorphisms (SNPs) associated with breast cancer (BC) risk. We investigated these and 62 other SNPs for their prognostic relevance. Confirmed BC risk rs17468277 (CASP8), rs1982073 (TGFB1), rs2981582 (FGFR2), rs13281615 (8q24), rs3817198 (LSP1), rs889312 (MAP3K1), rs3803662 (TOX3), rs13387042 (2q35), rs4973768 (SLC4A7), rs6504950 (COX11) rs10941679 (5p12) were genotyped 25 853 patients the available follow-up; SNPs,...
We evaluated the joint associations between a new 313-variant PRS (PRS313) and questionnaire-based breast cancer risk factors for women of European ancestry, using 72 284 cases 80 354 controls from Breast Cancer Association Consortium. Interactions were standard logistic regression newly developed case-only method overall by estrogen receptor status. After accounting multiple testing, we did not find evidence that per-standard deviation PRS313 odds ratio differed across strata defined...
To uncover mechanisms underlying chemotherapy-induced cognitive impairment in breast cancer, we studied new biomarkers of neuroinflammation and neuronal survival. This cohort study included 74 women (47 ± 10 years) from 22 October 2017 until 20 August 2020. Nineteen chemotherapy-treated 18 chemotherapy-naïve patients with cancer were assessed one month after the completion surgery and/or chemotherapy, 37 healthy controls included. Assessments neuropsychological testing, questionnaires, blood...
Abstract Research on metastatic cancer has been hampered by limited sample availability. Here we present the breast post-mortem tissue donation program UPTIDER and show how it enabled sampling of a median 31 (range: 5-90) metastases 5-8 liquids per patient from its first 20 patients. In dedicated experiment, mild impact increasing time after death RNA quality, transcriptional profiles immunohistochemical staining in tumor samples. We that this can be counteracted organ cooling. successfully...
The bicyclam AMD3100 is a highly potent and selective CXCR4 antagonist with strong antiviral activity against human immunodeficiency virus (HIV)-1 HIV-2, which use as coreceptor for host cell entry. Here, we investigated the interaction of at molecular level by mutational analysis. We established set stably transfected U87.CD4 lines expressing different mutant forms (i.e., CXCR4[WT], CXCR4[D171N], CXCR4[D262N], CXCR4[D171N,D262N], CXCR4[H281A]), to compare compound mutated versus wild-type...