A5022005227- Breast Cancer Treatment Studies
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Advanced Breast Cancer Therapies
- Breast Lesions and Carcinomas
- Lymphatic System and Diseases
- Cancer Risks and Factors
- Estrogen and related hormone effects
- Cancer Genomics and Diagnostics
- HER2/EGFR in Cancer Research
- Immune cells in cancer
- BRCA gene mutations in cancer
- Radiomics and Machine Learning in Medical Imaging
- Cancer Treatment and Pharmacology
- Prostate Cancer Treatment and Research
- Molecular Biology Techniques and Applications
- Cardiac Ischemia and Reperfusion
- Multiple and Secondary Primary Cancers
- Ferroptosis and cancer prognosis
- Cancer Diagnosis and Treatment
- Breast Implant and Reconstruction
- Adipose Tissue and Metabolism
- Cancer, Stress, Anesthesia, and Immune Response
- Diagnosis and Treatment of Venous Diseases
- Cardiac electrophysiology and arrhythmias
KU Leuven
2013-2024
Universitair Ziekenhuis Leuven
2017-2024
Cancer Institute (WIA)
2022
Inserm
2019
Agendia (Netherlands)
2019
Institut Curie
2019
Immunité et Cancer
2019
Breast Center
2018
AZ Sint-Lucas
2006-2017
Ghent University Hospital
2017
Abstract Since a detailed inventory of endothelial cell (EC) heterogeneity in breast cancer (BC) is lacking, here we perform single RNA-sequencing 26,515 cells (including 8433 ECs) from 9 BC patients and compare them to published EC taxonomies lung tumors. Angiogenic ECs are phenotypically similar, while other subtypes different. Predictive interactome analysis reveals known but also previously unreported receptor-ligand interactions between immune cells, suggesting an involvement responses....
Anti-HER2 antibody-drug conjugates (ADCs) have shown important efficacy in HER2-low metastatic breast cancer (mBC). Criteria for receiving ADCs are based on a single assay the primary tumour or small biopsy. We assessed intra-patient inter-metastasis heterogeneity of status HER2-negative mBC.We included samples 10 patients (7 ER-positive and 3 ER-negative) donated context our post-mortem tissue donation program UPTIDER. Excisional biopsies 257 metastases 8 tumours underwent central HER2...
Obesity is associated with an increased risk of developing breast cancer (BC) and worse prognosis in BC patients, yet its impact on biology remains understudied humans. This study investigates how the untreated primary differs according to patients' body mass index (BMI) using data from >2,000 patients. We identify several genomic alterations that are differentially prevalent overweight or obese patients compared lean report evidence supporting ageing accelerating effect obesity at genetic...
Importance Young women with breast cancer who have germline pathogenic variants in BRCA1 or BRCA2 face unique challenges regarding fertility. Previous studies demonstrating the feasibility and safety of pregnancy survivors included limited data BRCA carriers. Objective To investigate cumulative incidence disease-free survival young are Design, Setting, Participants International, multicenter, hospital-based, retrospective cohort study conducted at 78 participating centers worldwide. The...
When added to decongestive lymphatic therapy (DLT), what is the effect of fluoroscopy-guided manual drainage (MLD) versus traditional MLD or placebo for treatment breast cancer-related lymphoedema (BCRL)?Multicentre, three-arm, randomised controlled trial with concealed allocation, intention-to-treat analysis and blinding assessors participants.At five hospitals in Belgium, 194 participants unilateral chronic BCRL were recruited.All received standard DLT (education, skin care, compression...
Abstract Research on metastatic cancer has been hampered by limited sample availability. Here we present the breast post-mortem tissue donation program UPTIDER and show how it enabled sampling of a median 31 (range: 5-90) metastases 5-8 liquids per patient from its first 20 patients. In dedicated experiment, mild impact increasing time after death RNA quality, transcriptional profiles immunohistochemical staining in tumor samples. We that this can be counteracted organ cooling. successfully...
In most mammalian species force of contraction cardiac muscle increases with increasing rate stimulation, i.e. a positive force‐frequency relationship. single mouse ventricular cells, both and negative relationships have been described little is known about the underlying mechanisms. We studied enzymatically isolated myocytes, at 30 °C. During field amplitude unloaded cell shortening increased frequency stimulation ( 0.04 ± 0.01 Δ L / 0 1 Hz to 0.07 4 Hz, n = 12, P < 0.05 ). whole voltage...
Abstract Limited data exist regarding the associations between TROP-2 protein expression, clinical–pathological characteristics, and outcome in triple-negative breast cancer (TNBC). expression was determined for patients diagnosed with TNBC 2000 2017 by immunohistochemistry (IHC) (ab227689, Abcam) on whole slide tumor sections, assessed as continuous categorical variables (H-score high, 201–300, medium 100–200 low < 100). We investigated prognostic value of relapse survival, baseline...
Primary tumors with a mixed invasive breast carcinoma of no-special type (IBC-NST) and lobular cancer (ILC) histology are present in approximately five percent all patients understudied at the metastatic level. Here, we characterized metastases from two primary IBC-NST/ILC postmortem tissue donation program UPTIDER (NCT04531696). The 14 43 lesions collected autopsy had morphological features E-cadherin staining patterns consistent pure ILC. While our findings still require further...
Type II diabetes, often associated with abdominal obesity, frequently leads to heart failure. Clinical and epidemiological evidence suggests that supplemental dyslipidaemia hypertension, as clustered in the metabolic syndrome, aggravate cardiovascular outcome. The differential impact of type diabetes syndrome on left ventricular function, however, remains incompletely defined. We studied function vivo using pressure–volume analysis obese diabetic mice leptin deficiency (ob/ob) dyslipidemic...
Background: Lymphedema is associated with significant physical and psychosocial problems. The Functioning, Disability Health questionnaire for upper limb lymphedema a valid reliable tool quantifying the amount of problems in functioning patients breast cancer-related lymphedema. Patients suggested revision scoring system to facilitate completion questionnaire. Therefore, adjustment was carried out by implementing numeric rating scale instead existing visual analog scale. Purpose this study...
To investigate the reliability, time efficiency and clinical feasibility of five commonly used methods for assessing excessive arm volume in patients with breast cancer-related lymphoedema (BCRL).Cross-sectional study.University Hospitals Leuven, Belgium.30 participants unilateral BCRL.Excessive was determined by different methods: traditional volumetry overflow, without inverse volumetry, optoelectronic calculated based on circumference measurements. intra- inter-rater measurements were...
The objective of this trial was to investigate the effectiveness fluoroscopy-guided manual lymph drainage (MLD), as part decongestive lymphatic therapy (DLT), on superficial architecture in patients with chronic mild moderate breast cancer-related lymphoedema (BCRL). This a multicentre, double-blind, randomised controlled involving 194 participants BCRL. Participants were into (1) DLT MLD (intervention group), (2) traditional (control or (3) placebo (placebo group). Superficial evaluated...
Background: Local tissue water in patients with breast cancer-related lymphedema (BCRL) can be assessed by measurement of the dielectric constant using MoistureMeterD Compact® (MMDC) device, or performing pitting test. Although these assessment methods are commonly used clinical practice, literature shows a lack research on their clinimetric properties. Therefore, aim this study was to investigate reliability both methods, assessing upper limb BCRL. Methods and Results: Thirty women BCRL...
<b><i>Aims:</i></b> Insulin resistance, dyslipidemia and hypertension are independent mediators of endothelial dysfunction. It is incompletely defined whether in addition to diabetes mellitus type II (DMII), as seen the metabolic syndrome (MS), worsen diabetes-induced Furthermore, it unclear treatment influences dysfunction similarly MS DMII. Therefore, we studied vascular reactivity effect vivo with angiotensin-converting enzyme inhibition (ACE-I) or hypocaloric diet...
Background: A disturbance of the superficial lymphatic system (dermal backflow) in patients with breast cancer-related lymphedema (BCRL) can be visualized by near-infrared fluorescence imaging or lymphofluoroscopy. In clinical practice, exact measurement dermal backflow is difficult. The purpose study to investigate concurrent validity between assessments and lymphofluoroscopy BCRL. Methods Results: Forty-five BCRL stage I IIb received their edematous limb (pitting status, skinfold...