A5067596998- PI3K/AKT/mTOR signaling in cancer
- Pancreatic function and diabetes
- Cancer, Lipids, and Metabolism
- MicroRNA in disease regulation
- Liver physiology and pathology
- Immune Cell Function and Interaction
- Liver Disease Diagnosis and Treatment
- RNA modifications and cancer
- Nutrition, Genetics, and Disease
- Pharmacogenetics and Drug Metabolism
- Genetic and Kidney Cyst Diseases
- Metabolism, Diabetes, and Cancer
- Cholesterol and Lipid Metabolism
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Immune responses and vaccinations
- Inflammasome and immune disorders
- Genomics, phytochemicals, and oxidative stress
- Immune cells in cancer
- T-cell and B-cell Immunology
- Mitochondrial Function and Pathology
Spanish National Cancer Research Centre
2019-2025
Centro de Investigación del Cáncer
2025
KU Leuven
2022-2023
VIB-KU Leuven Center for Cancer Biology
2022
Durham University
2015
Abstract The metabolic functions of the liver are spatially organized in a phenomenon called zonation, linked to differential exposure portal and central hepatocytes nutrient-rich blood. mTORC1 signaling pathway controls cellular metabolism response nutrients insulin fluctuations. Here we show that simultaneous genetic activation nutrient hormone results impaired establishment postnatal zonal identity hepatocytes. Mutant fail upregulate postnatally expression Frizzled receptors 1 8, reduced...
The mechanistic target of rapamycin complex 1 (mTORC1) integrates cellular nutrient signaling and hormonal cues to control metabolism. We have previously shown that constitutive mTORC1 by means genetic activation RagA (expression GTP-locked RagA, or RagAGTP) in mice resulted a fatal energetic crisis at birth. Herein, we rescue neonatal lethality RagAGTP find morphometric metabolic alterations span glucose, lipid, ketone, bile acid amino homeostasis adults, median lifespan nine months....
We present a protocol for measuring the activity of mechanistic target rapamycin (mTOR) pathway in ex vivo isolated mouse primary hepatocytes. It can be used as tool genetic, pharmacological, metabolomic, and signal transduction procedures. discuss critical aspects improving yield, viability, modulation mTOR pathway. This adapted to other signaling cascades is compatible with multiple readouts. For complete details on use execution this protocol, please refer Ortega-Molina et al. (2021).
Conditions are described for the preparation of cholesterol with 17O and 18O labels from i-cholesteryl methyl ether using minimal amounts isotopically enriched water. Optimum yields employed trifluoromethanesulfonic acid as catalyst in 1,4-dioxane at room temperature 5 equivalents An isotopic enrichment >90% that water used reaction could be attained. Tetrafluoroboric also catalyst, expense a lower overall yield. Byproducts included dicholesteryl ether, cholesteryl compounds formed by...
Abstract Cancer cells outgrowing in distant organs of metastasis rewire their metabolism to fuel on the available nutrients. While this is often considered an adaptive pressure limiting formation, some nutrients at metastatic site naturally or through changes organ physiology may inherently promote growth. We find that lung, a frequent metastasis, lipid-rich environment. Moreover, we observe pathological conditions such as pre-metastatic niche formation and obesity further increase...