A5022837689- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Neuroinflammation and Neurodegeneration Mechanisms
- Carbohydrate Chemistry and Synthesis
- Antimicrobial Peptides and Activities
- Chemical Synthesis and Analysis
- Aquaculture Nutrition and Growth
- Metabolomics and Mass Spectrometry Studies
- Inflammation biomarkers and pathways
- Antibiotics Pharmacokinetics and Efficacy
- Pneumonia and Respiratory Infections
- Click Chemistry and Applications
- Advanced Polymer Synthesis and Characterization
- Eicosanoids and Hypertension Pharmacology
- Immune cells in cancer
- Glycosylation and Glycoproteins Research
- Algal biology and biofuel production
- Supramolecular Self-Assembly in Materials
- Surfactants and Colloidal Systems
- Nitric Oxide and Endothelin Effects
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
National Research Council
2021-2024
Technobiochip (Italy)
2022
Consorzio Roma Ricerche
2022
Istituto di Chimica Biomolecolare
2021
Institute of Protein Biochemistry
2017-2019
University of Naples Federico II
2019
The immune response arises from a fine balance of mechanisms that provide for surveillance, tolerance, and elimination dangers. Sulfavant A (SULF A) is sulfolipid with promising adjuvant activity. Here we studied the mechanism action SULF addressed identification its molecular target in human dendritic cells (hDCs).Adjuvant effect immunological to were assessed on DCs derived donors. In addition testing various reporter cells, downstream signalling was supported by reverse pharmacology...
Adjuvants are components of vaccine that enhance the specific immune response against co-inoculated antigens. Recently, we reported characterization a synthetic sulfolipid named Sulfavant A (1) as promising candidate novel class molecular adjuvants based on sulfoquinovosyl-diacylglycerol skeleton. Here, report an improved synthesis scaffold, well preparation two analogs Sulfavant-S (2) and Sulfavant-R (3) with enhanced property to modulate master targets such human dendritic cells (DCs)....
Introduction Sulfavant A (SULF A) is a synthetic derivative of naturally occurring sulfolipids. The molecule triggers TREM2-related maturation dendritic cells (DCs) and has shown promising adjuvant activity in cancer vaccine model. Methods the immunomodulatory SULF tested an allogeneic mixed lymphocyte reaction (MLR) assay based on monocyte-derived naïve T lymphocytes from human donors. Flow cytometry multiparametric analyses ELISA assays were performed to characterize immune populations,...
Vaccines play a primary role in the protection of human health by preventing infectious and chronic diseases. Recently we have reported 1,2-O-distearoyl-3-O-β-d-sulfoquinovosylglycerol (β-SQDG18), here named Sulfavant A (1), which shows promising properties as new molecular adjuvant vitro vivo tests. In present manuscript, provide full details about synthetic strategy for preparation 1, including discussion chemical determinants activity major technical hurdles faced during study. Synthesis...
Immunotherapy takes advantage of the immune system to prevent, control, and eliminate neoplastic cells. The research in field has already led major breakthroughs treat cancer. In this work, we describe a platform that integrates
Recently, we described synthetic sulfolipids named Sulfavants as a novel class of molecular adjuvants based on the sulfoquinovosyl-diacylglycerol skeleton. The members this family, Sulfavant A (1), R (2), and S (3), showed important effects triggering receptor expressed myeloid cells 2 (TREM2)-induced differentiation maturation human dendritic (hDC), through cell mechanism underlying regulation immune response. As these molecules are involved in biological TREM2-mediated processes crucial...
In aqueous conditions, amphiphilic bioactive molecules are able to form self-assembled colloidal structures modifying their biological activity. This behavior is generally neglected in preclinical studies, despite its impact on pharmacological development. this regard, a significative example represented by new class of marine-inspired vaccine adjuvants, collectively named Sulfavants, based the β-sulfoquinovosyl-diacylglyceride skeleton. The family includes lead product Sulfavant A (1) and...
Recently, we reported the promising activity of a novel class sulfoquinovosyl-diacylglycerols named Sulfavants as molecular vaccine adjuvants. Herein, describe modified and improved chemical synthesis lead product Sulfavant A (1), with aim to produce from milligrams 10 g pure compound that is necessary for preclinical development. Starting versatile based on trichloroacetimidate methodology, up-scaled preparation (1) was achieved in 11 steps by elimination modification those reactions...
Microalgae are currently considered an attractive source of highly valuable metabolites potentially exploitable as anticancer agents, nutraceuticals and cosmeceuticals for bioenergy purposes. Their ease culturing their high growth rates further promote use raw material the production specialty products. In present paper, we focused our attention on specific glycerol-based lipid compounds, monoacylglycerols (MAGs), which displayed in previous studies a selective cytotoxic activity against...
Abstract The immune response arises from a fine balance of cellular and molecular mechanisms that provide for surveillance, tolerance, elimination dangers as pathogens. Improving the quality remains major goal in immunotherapy vaccine development. Sulfavant A (SULF A) is sulfolipid has shown promising adjuvant activity cancer model. Here we report SULF first synthetic small molecule binding to Triggering Receptor Expressed on Myeloid cells-2 (TREM2). receptor engagement initiates an...
A fast and sensitive method that is based on Ultra High Performance Liquid Chromatography coupled with Resolution Mass Spectrometry (UHPLC-HRMS) for the measurement of Sulfavant A, a molecular adjuvant sulfolipid skeleton, described. The has been validated over linearity range 2.5–2000 ngmL−1 using deuterated derivative (d70-Sulfavant A) as internal standard. Chromatographic separation UHPLC Kinetex® 2.6 µm PS C18 column gradient methanol in 0.32 mM ammonium hydroxide solution buffered at pH...