A5071682725- Reproductive System and Pregnancy
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Advanced Polymer Synthesis and Characterization
- Nail Diseases and Treatments
- Plant and animal studies
- Insect and Arachnid Ecology and Behavior
- Genetic and Kidney Cyst Diseases
- Medical and Biological Ozone Research
- Protist diversity and phylogeny
- Luminescence and Fluorescent Materials
- Plant Pathogens and Fungal Diseases
- Fungal Infections and Studies
- Genetics and Neurodevelopmental Disorders
- Supramolecular Chemistry and Complexes
- Proteoglycans and glycosaminoglycans research
- Extracellular vesicles in disease
- Hydrogen's biological and therapeutic effects
- Micro and Nano Robotics
- Nanoparticles: synthesis and applications
- Photochromic and Fluorescence Chemistry
- Immune Cell Function and Interaction
- Endometriosis Research and Treatment
- Fossil Insects in Amber
- Osteoarthritis Treatment and Mechanisms
University of Siena
2015-2024
Walter de Gruyter (Germany)
2015
Robert Koch Institute
2015
Goethe University Frankfurt
2015
Institute for Macromolecular Studies
2005-2008
Kanagawa Academy of Science and Technology
2005
Intraflagellar transport (IFT) is the bidirectional movement of multipolypeptide particles between ciliary membrane and axonemal microtubules, required for assembly, maintenance, sensory function cilia flagella. In this paper, we present first high-resolution ultrastructural analysis trains flagellar IFT particles, using transmission electron microscopy electron-tomographic sections from flat-embedded Chlamydomonas reinhardtii cells. Using wild-type mutant cells with defects in IFT,...
Alkaptonuria (AKU) is an ultra-rare disease developed from the lack of homogentisic acid oxidase activity, causing (HGA) accumulation that produces a HGA-melanin ochronotic pigment, unknown composition. There no therapy for AKU. Our aim was to verify if AKU implied secondary amyloidosis. Congo Red, Thioflavin-T staining and TEM were performed assess amyloid presence in specimens (cartilage, synovia, periumbelical fat, salivary gland) HGA-treated human chondrocytes cartilage. SAA SAP...
Evidence of oxidative stress has been reported in the blood patients with Rett syndrome (RTT), a neurodevelopmental disorder mainly caused by mutations gene encoding Methyl-CpG-binding protein 2. Little is known regarding redox status RTT cellular systems and its relationship morphological phenotype. In ( n = 16) we investigated four different markers, F 2 -Isoprostanes (F -IsoPs), 4 -Neuroprostanes -NeuroPs), nonprotein bound iron (NPBI), (4-HNE PAs), glutathione one most accessible cells,...
Intraflagellar transport (IFT) is responsible for the bidirectional trafficking of molecular components required elongation and maintenance eukaryotic cilia flagella. Cargo transported by IFT 'trains', linear rows multiprotein particles moved motors along axonemal doublets. We have previously described two structurally distinct categories 'long' 'short' trains. Here, we analyse relative number these trains throughout flagellar regeneration show that long are most abundant at beginning growth...
Background . Alkaptonuria, a rare autosomal recessive metabolic disorder caused by deficiency in homogentisate 1,2-dioxygenase activity, leads to accumulation of oxidised homogentisic acid cartilage and collagenous structures present all organs tissues, especially joints heart, causing pigmentation called ochronosis. A secondary amyloidosis is associated with AKU. Here we report study an aortic valve from AKU patient. Results Congo Red birefringence, Th-T fluorescence, biochemical assays...
Near-infrared quantum dots (NIR QDs) are a new class of fluorescent labels with excellent bioimaging features, such as high fluorescence intensity, good stability, sufficient electron density, and strong tissue-penetrating ability. For all NIR QDs have great potential for early cancer diagnosis, in vivo tumor imaging resolution microscopy studies on cells.In the present study we constructed functionalized NT4 cancer-selective tetrabranched peptides (NT4-QDs). We observed specific uptake...
Embryo implantation has been defined as the “black box” of human reproduction. Most knowledge on mechanisms underlining this process derives from animal models, but they cannot always be translated to humans. Therefore, development an in vitro/ex vivo model recapitulating closely and precisely possible fundamental functional features endometrial tissue is very much desirable. Here, we have validated organoids a suitable 3D-model studying epithelial interface for embryo implantation....
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) may affect female reproductive health. Here, we investigated the potential of SARS-CoV-2 to infect follicular microenvironment, in particular granulosa (GCs) and cumulus cells (CCs), thus providing evidence for a productive infection. GCs CCs were recovered from women (n = 25) who underwent vitro fertilization at Assisted Reproductive Unit, Siena University Hospital. Follicular ovarian co-cultured with then analyzed by qPCR,...
Despite the significant advances in last decades, low implantation rate per transferred embryo still remains a major concern assisted reproductive techniques, highlighting need to better characterize endometrial receptivity also by mean of specific biomarkers. Based on physiology and intimate contact with endometrium as tissue interest, this study we developed validated an optimized protocol that uses extracellular vesicles (EVs) recovered from uterine flushings cervical brush, latter never...
Surface capping rules AgNP dissolution and interaction with biomolecules cells, influencing the exposure outcome.
Objective: To analyse whether an HIV-1 envelope protein might play a role in damaging the blood-brain barrier as fundamental step early invasion of central nervous system by HIV-1. Design: Analysis permeability rat brain endothelium cultures to albumin, assess functional integrity vascular component barrier. Methods: Rat prepared cerebral microvessels were exposed recombinant gp120IIIB on microporous membranes and passage biotin-labelled albumin was analysed. Scanning electron microscopy...
Vinyl polymers carrying l-phenylalanine residues were synthesized in the free and cross-linked hydrogel form, as homo- copolymers with N-isopropylacrylamide (NIPAAm). The protonation reaction thermodynamics of COO- group studied aqueous media, at different temperatures increased concentrations sodium chloride, mainly by potentiometry solution calorimetry. soluble polymer, namely poly(N-acryloyl-l-phenylalanine), its low NIPAAm content displayed characteristic features. Their basicity...
The synthesis of a series benzofulvene derivatives 3 related to the recently studied ethyl 1-methylene-3-(4-methylphenyl)-1H-indene-2-carboxylate (BF1) is described. properties these trans-diene were characterized with regard their capability polymerizing spontaneously give new polymers based on functionalized indene monomeric units. has been investigated by NMR spectroscopy, multiangle light scattering online size exclusion chromatography, UV−vis mass spectrometry, differential scanning...
Autism spectrum disorders (ASDs) are a complex group of neurodevelopment steadily rising in frequency and treatment refractory, where the search for biological markers is paramount importance. Although red blood cells (RBCs) membrane lipidomics rheological variables have been reported to be altered, with some suggestions indicating an increased lipid peroxidation erythrocyte membrane, date no information exists on how oxidative damage may affect cytoskeletal proteins and, ultimately, RBCs...
Abstract The controversial mating of the strepsipteran Xenos vesparum was studied to investigate possible sperm routes for fertilization. female, which is a neotenic permanent endoparasite Polistes wasps, extrudes only its anterior region, “cephalothorax,” from host abdomen. This region has an opening where both and larval escape occur. Observations with scanning transmission electron microscopy revealed spermatozoa not in hemocoel, but also “ventral canal” (an extragenital duct peculiar...
The polymerization of trans-diene BF1 [ethyl 1-methylene-3-(4-methylphenyl)-1H-indene-2-carboxylate] has been studied in the presence various amounts an anionic initiator such as phenyllithium order to obtain information on properties this diene monomer and its polymers. produced a mixture oligomers polymer (poly-BF1-AP), proportion which is regulated by amount used. Poly-BF1 was separated from lower basis solubility n-hexane, soluble material further fractionated chromatography activated...