A5040409553- Multiple Myeloma Research and Treatments
- Protein Degradation and Inhibitors
- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Chemokine receptors and signaling
- Advanced biosensing and bioanalysis techniques
- Genetic Syndromes and Imprinting
- Zebrafish Biomedical Research Applications
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- Chromosomal and Genetic Variations
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Pluripotent Stem Cells Research
- Peptidase Inhibition and Analysis
- Cancer Mechanisms and Therapy
- PI3K/AKT/mTOR signaling in cancer
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- Lymphoma Diagnosis and Treatment
Center for Excellence in Molecular Cell Science
2020-2024
Chinese Academy of Sciences
2008-2024
University of Chinese Academy of Sciences
2020-2024
University of Macau
2024
Zhengzhou University
2022-2024
Harvard University
2014-2022
Dana-Farber Cancer Institute
2015-2022
Henan Provincial People's Hospital
2022
Henan University
2022
Brigham and Women's Hospital
2017
Purpose Clonal hematopoiesis of indeterminate potential (CHIP) is an age-related condition characterized by somatic mutations in the blood otherwise healthy adults. We hypothesized that patients undergoing autologous stem-cell transplantation (ASCT) for lymphoma, CHIP at time ASCT would be associated with increased risk myelodysplastic syndrome and acute myeloid leukemia, collectively termed therapy-related neoplasm (TMN), other adverse outcomes. Methods performed whole-exome sequencing on...
Highlights•CXCR4 mediates extra-medullary multiple myeloma (MM)•CXCR4 enhances EMT-like phenotype acquisition in MM•Ulocuplumab inhibits MM cell dissemination and EMT MMSummaryExtra-medullary disease (EMD) (MM) is associated with poor prognosis resistance to chemotherapy. However, molecular alterations that lead EMD have not been well defined. We developed bone marrow (BM)- EMD-prone syngeneic lines; identified epithelial-to-mesenchymal transition (EMT) transcriptional patterns were...
The development of sensitive and non-invasive "liquid biopsies" presents new opportunities for longitudinal monitoring tumor dissemination clonal evolution. number circulating cells (CTCs) is prognostic in multiple myeloma (MM), but there little information on their genetic features. Here, we have analyzed the genomic landscape CTCs from 29 MM patients, including eight cases with matched/paired bone marrow (BM) cells. Our results show that 100% mutations patient BM were detected 99% present...
Despite significant advances in the treatment of multiple myeloma (MM), most patients succumb to disease progression. One major immunosuppressive mechanisms that is believed play a role progression expansion regulatory T cells (Tregs). In this study, we demonstrate drive Treg and activation by secreting type 1 interferon (IFN). Blocking IFN α β receptor (IFNAR1) on Tregs significantly decreases both myeloma-associated function Using syngeneic transplantable murine models bone marrow (BM)...
Targeting translation initiation in multiple myeloma induces inhibition of a specific oncogenic program.
Abstract Background Peripheral arterial disease (PAD), a major manifestation of atherosclerosis, is associated with significant cardiovascular morbidity, limb loss and death. However, mechanisms underlying the genesis progression are far from clear. Genome-wide gene expression profiling clinical samples may represent an effective approach to gain relevant information. Results After histological classification, total 30 femoral artery samples, including 11 intermediate lesions, 14 advanced...
Advancement of many solid tumors and hematologic malignancies is frequently characterized by dissemination homing cancer cells to the bone marrow (BM). Methods quantitatively characterize these key steps metastatic cascade in mammalian models are currently limited do not offer opportunities perform rapid, large-scale genomic, or drug screening. Because their optical clarity, we used zebrafish develop an vivo model cell BM. We performed intracardiac injection multiple myeloma (MM) derived...
Identifying the primary site of metastatic cancer is critical to guiding subsequent treatment. Approximately 3-9% patients are diagnosed with unknown sites (CUP) even after a comprehensive diagnostic workup. However, widely accepted molecular test still not available. Here, we report method that applies formalin-fixed, paraffin-embedded tissues construct reduced representation bisulfite sequencing libraries (FFPE-RRBS). We then generate and systematically evaluate 28 classifiers, built on...
Next-generation sequencing platforms for measuring digital expression such as RNA-Seq are displacing traditional microarray-based methods in biological experiments. The detection of differentially expressed genes between groups conditions has led to the development numerous bioinformatics tools, but so far, few exploit expanded dynamic range afforded by new technologies. We present edgeRun, an R package that implements unconditional exact test is a more powerful version edgeR. This increase...
Abstract Background Gestational diabetes mellitus (GDM) is a common pregnancy-specific disease and growing at an alarming rate worldwide, which can negatively affect the health of pregnant women fetuses. However, most studies are limited to one tissue, placenta or umbilical cord blood, usually with omics assay. It thus difficult systematically reveal molecular mechanism GDM key influencing factors on offspring. Results We recruited group 21 20 controls without GDM. For each woman, reduced...
Pharmacological intervention of redox balance in cancer cells often results oxidative stress-mediated apoptosis, attracting much attention for the development a new generation targeted therapy cancer. However, little is known about mechanisms underlying conversion from signaling to downstream activities leading death.We here report systematic detection transcriptome changes response signals generated leukemia upon fenretinide treatment, implicating occurrence numerous stress-responsive...
Chromosomes occupy discrete spaces in the interphase cell nucleus, called chromosome territory. The structural and functional relevance of territory remains elusive. We fused 15 17 mouse haploid embryonic stem cells (haESCs), resulting distinct changes territories cognate chromosomes, but with little effect on gene expression, pluripotency gamete functions haESCs. karyotype-engineered haESCs were successfully implemented generating heterozygous (2n = 39) homozygous 38) models. Mice...
Motif discovery is essential for deciphering regulatory codes from high throughput genomic data, such as those ChIP-chip/seq experiments. However, there remains a lack of effective and efficient methods the identification long gapped motifs in many relevant tools reported to date. We describe here an automated tool that allows de novo transcription factor binding sites, regardless whether are or short, contiguous.