A5016056400- Chronic Lymphocytic Leukemia Research
- CRISPR and Genetic Engineering
- Cytokine Signaling Pathways and Interactions
- Acute Lymphoblastic Leukemia research
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Advanced Breast Cancer Therapies
- Cancer Genomics and Diagnostics
- Multiple Myeloma Research and Treatments
- Genomics and Chromatin Dynamics
- Immunodeficiency and Autoimmune Disorders
- Protein Degradation and Inhibitors
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Chronic Myeloid Leukemia Treatments
- Innovation and Socioeconomic Development
- Cytomegalovirus and herpesvirus research
- CAR-T cell therapy research
- Cancer therapeutics and mechanisms
- Reproductive System and Pregnancy
- Genetic Syndromes and Imprinting
- Virus-based gene therapy research
- Pancreatic function and diabetes
- RNA regulation and disease
University of Utah
2024-2025
Center for Cancer Research
2018-2024
Harvard University
2014-2024
Massachusetts General Hospital
2017-2024
Kingston University
2024
Broad Institute
2014-2023
Eli and Edythe Broad Foundation
2022
Harvard Stem Cell Institute
2015-2019
Harvard–MIT Division of Health Sciences and Technology
2014-2015
Massachusetts Institute of Technology
2014
Recent innovations in single-cell Assay for Transposase Accessible Chromatin using sequencing (scATAC-seq) enable profiling of the epigenetic landscape thousands individual cells. scATAC-seq data analysis presents unique methodological challenges. experiments sample DNA, which, due to low copy numbers (diploid humans), lead inherent sparsity (1-10% peaks detected per cell) compared transcriptomic (scRNA-seq) (10-45% expressed genes cell). Such challenges generation emphasize need informative...
Abstract Sequencing-based approaches have led to new insights about DNA methylation. While many different techniques for genome-scale mapping of methylation been employed, throughput has a key limitation most. To further facilitate the methylation, we describe protocol gel-free multiplexed reduced representation bisulfite sequencing (mRRBS) that reduces workload dramatically and enables processing 96 or more samples per week. mRRBS achieves similar CpG coverage original RRBS protocol, while...
In normal mammalian development cytosine methylation is essential and directed to specific regions of the genome. Despite notable advances through mapping its genome-wide distribution, studying direct contribution DNA gene genome regulation has been limited by lack tools for precise manipulation. Thus, combining targeting capability CRISPR-Cas9 system with an epigenetic modifier attracted interest in scientific community. contrast profiling cleavage a nuclease competent Cas9, tracing global...
Abstract Inflammatory breast cancer (IBC) is a difficult-to-treat disease with poor clinical outcomes due to high risk of metastasis and resistance treatment. In cancer, CD44+CD24− cells possess stem cell-like features contribute progression, we previously described CD44+CD24−pSTAT3+ cell subpopulation that dependent on JAK2/STAT3 signaling. Here report are the most frequent type in IBC commonly pSTAT3+. Combination inhibition paclitaxel decreased xenograft growth more than either agent...