A5026875941- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Genomics, phytochemicals, and oxidative stress
- CRISPR and Genetic Engineering
- Renal and related cancers
- Dermatology and Skin Diseases
- Cancer-related gene regulation
- Tissue Engineering and Regenerative Medicine
- Muscle Physiology and Disorders
- RNA modifications and cancer
- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Digestive system and related health
- Cancer Cells and Metastasis
- Hair Growth and Disorders
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Kruppel-like factors research
- Skin and Cellular Biology Research
- Educational Methods and Impacts
- Neonatal Respiratory Health Research
- Chromosomal and Genetic Variations
- Microtubule and mitosis dynamics
- Innovative Teaching Methods
- Plant Molecular Biology Research
University of California, San Francisco
2025
Massachusetts General Hospital
2015-2024
Harvard University
2015-2024
Harvard Stem Cell Institute
2015-2024
Broad Institute
2021
Massachusetts Institute of Technology
2021
Howard Hughes Medical Institute
2015-2016
University of Colorado Anschutz Medical Campus
2012-2016
ProHealth Oconomowoc Memorial Hospital
2016
University of Colorado Denver
2011-2014
Skeletal muscle harbors quiescent stem cells termed satellite and proliferative progenitors myoblasts, which play pivotal roles during regeneration. However, current technology does not allow permanent capture of these cell populations in vitro. Here, we show that ectopic expression the myogenic transcription factor MyoD, combined with exposure to small molecules, reprograms mouse fibroblasts into expandable induced progenitor (iMPCs). iMPCs express key skeletal markers including Pax7 Myf5...
The transcription factor Nrf2 is a key regulator of the cellular stress response, and pharmacological activation promising strategy for skin protection cancer prevention. We show here that prolonged in keratinocytes causes sebaceous gland enlargement seborrhea mice due to upregulation growth epigen, which we identified as novel target. This was accompanied by thickening hyperkeratosis hair follicle infundibula. These abnormalities caused dilatation infundibula, loss, cyst development upon...
Sox2 expression marks gastric stem and progenitor cells, raising important questions regarding the genes regulated by role of itself during stomach homeostasis disease. By using ChIP-seq analysis, we have found that majority targets in epithelial cells are tissue specific related to functions such as endoderm development, Wnt signaling, cancer. Unexpectedly, is dispensable for cell self-renewal, yet Sox2(+) highly susceptible tumorigenesis an Apc/Wnt-driven mouse model. Moreover, loss...
Development of benchtop, portable Fourier transform nuclear magnetic resonance (NMR) and infrared (IR) spectrometers has opened up opportunities for creating university–high school partnerships that provide high students with hands-on experience NMR IR instruments. With recent changes to the international baccalaureate chemistry advanced placement curricula, spectroscopy can be mapped directly content is already built into general, organic, materials courses offered at level. We developed a...
The generation of myotubes from fibroblasts upon forced MyoD expression is a classic example transcription factor-induced reprogramming. We recently discovered that additional modulation signaling pathways with small molecules facilitates reprogramming to more primitive induced myogenic progenitor cells (iMPCs). Here, we dissected the transcriptional and epigenetic dynamics mouse undergoing either or iMPCs using MyoD-inducible transgenic model. Induction in combined generated Pax7 + high...
Epigenetic clocks, built on DNA methylation patterns of bulk tissues, are powerful age predictors, but their biological basis remains incompletely understood. Here, we conducted a comparative analysis epigenetic in murine muscle, epithelial, and blood cell types across lifespan. Strikingly, our results show that cellular subpopulations within these including adult stem progenitor cells as well differentiated progeny, exhibit different ages. Accordingly, experimentally demonstrate clocks can...
Barrett's esophagus (BE) and gastric intestinal metaplasia are related premalignant conditions in which areas of human stomach epithelium express mixed features. Intestinal transcription factors (TFs) expressed both conditions, with unclear causal roles cis-regulatory mechanisms. Ectopic CDX2 reprogrammed isogenic mouse organoid lines to a hybrid stomach-intestinal state transcriptionally similar clinical metaplasia; squamous esophageal organoids resisted this CDX2-mediated effect....
Aurora kinase-A (Aurora-A) promotes timely entry into mitosis, centrosome maturation, and formation of bipolar spindles. To address the role Aurora-A in skin development homeostasis, we interbred a floxed (Aurora-Afl) mouse with Cre-deleter strain, K14.Cre. Aurora-Afl/fl;Krt14.Cre (Aurora-A−/−) mice died shortly after birth. These had translucent skin, histological evaluation showed that dorsal was very thin fragile frank erosions. Although expression basal layer marker keratin 14...
Spermatogenesis requires progression of a self-renewing male germline stem cell population through precisely timed and ordered developmental sequence to form spermatozoa. For several years, we have been investigating the functional impact signals on this by members TGFβ superfamily, follicle stimulating hormone bcl-2 family. example, our lab others shown that activin A, bone morphogenetic protein (BMP)-4 glial-derived neurotropic factor (GDNF) all modulate development spermatogonial...