A5045317471- Congenital heart defects research
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Congenital Heart Disease Studies
- Epigenetics and DNA Methylation
- Genomics and Rare Diseases
- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- Genomic variations and chromosomal abnormalities
- RNA Research and Splicing
- Cardiomyopathy and Myosin Studies
- Genetic diversity and population structure
- SARS-CoV-2 and COVID-19 Research
- Physiological and biochemical adaptations
- Evolution and Genetic Dynamics
- Peptidase Inhibition and Analysis
- Gene Regulatory Network Analysis
- PARP inhibition in cancer therapy
- Protein Degradation and Inhibitors
- Renal and related cancers
- Bacterial biofilms and quorum sensing
- Mitochondrial Function and Pathology
- COVID-19 Clinical Research Studies
- Muscle Physiology and Disorders
Lucile Packard Children's Hospital
2021-2025
Stanford University
2022-2024
Palo Alto University
2024
Gladstone Institutes
2015-2023
Stanford Medicine
2022
University of California, San Francisco
2015-2019
Harvard University
2006-2018
Harvard Stem Cell Institute
2012-2016
Broad Institute
2012-2016
Boston University
2007
Bacterial pathogens frequently use protein secretion to mediate interactions with their hosts. Here we found that a virulence locus (HSI-I) of Pseudomonas aeruginosa encodes apparatus. The apparatus assembled in discrete subcellular locations and exported Hcp1, hexameric forms rings 40 angstrom internal diameter. Regulatory patterns HSI-I suggested the functions during chronic infections. We detected Hcp1 pulmonary secretions cystic fibrosis (CF) patients Hcp1-specific antibodies sera. Thus,...
Complex genetic mechanisms are thought to underlie many human diseases, yet experimental proof of this model has been elusive. Here, we show that a cardiac anomaly can be caused by combination rare, inherited heterozygous mutations. Whole-exome sequencing nuclear family revealed three offspring with childhood-onset cardiomyopathy had missense single-nucleotide variants in the
The evolution of reproductive barriers is the first step in formation new species and can help us understand diversification life on Earth. These often take form hybrid incompatibilities, which alleles derived from two different no longer interact properly hybrids
Mapping the gene-regulatory networks dysregulated in human disease would allow design of network-correcting therapies that treat core mechanism. However, small molecules are traditionally screened for their effects on one to several outputs at most, biasing discovery and limiting likelihood true disease-modifying drug candidates. Here, we developed a machine-learning approach identify broadly correct gene induced pluripotent stem cell (iPSC) model common form heart involving aortic valve...
Significance Spinal cord injury (SCI) significantly disrupts normal neural circuitry, leading to severe degradation of motor and sensory function. Excitatory interneurons that relay signals from the brain networks throughout spinal cord, including glutamatergic V2a coordinate respiration locomotion, are lost after SCI. Thus, transplantation SCI could provide a novel therapy restore functional connections between spared downstream neurons. This study describes generation human pluripotent...
Following injury, adult skeletal muscle undergoes a well-coordinated sequence of molecular and physiological events to promote repair regeneration. However, thorough understanding the in vivo epigenomic transcriptional mechanisms that control these reparative is lacking. To address this, we monitored dynamics three histone modifications coding noncoding RNA expression throughout regenerative process mouse model traumatic injury. We first illustrate how both RNAs tissues sorted satellite...
There is considerable variability in the susceptibility and progression for COVID-19 it appears to be strongly correlated with age, gender, ethnicity pre-existing health conditions. However, our knowledge, cohort studies of clinically vulnerable groups are lacking. Host genetics has also emerged as a major risk factor COVID-19, variation ACE2 receptor, which facilitates entry SARS-CoV-2 virus into cell, become focus attention. Thus, we interrogated an ethnically diverse National Health...
Abstract The evolution of reproductive barriers is the first step in formation new species and can help us understand diversification life on Earth. These often take form “hybrid incompatibilities,” where alleles derived from two different no longer interact properly hybrids. Theory predicts that hybrid incompatibilities may be more likely to arise at rapidly evolving genes involving multiple should common, but there has been sparse empirical data evaluate these predictions. Here, we...
GATA4 (GATA-binding protein 4), a zinc finger-containing, DNA-binding transcription factor, is essential for normal cardiac development and homeostasis in mice humans, mutations this gene have been reported human heart defects. Defects alternative splicing are associated with many diseases, yet relatively little known about how cell type- or state-specific achieved the heart. Here, we show that regulates type-specific through direct interaction RNA spliceosome induced pluripotent stem...
Abstract Traumatic lower-limb musculoskeletal injuries are pervasive amongst athletes and the military typically an individual returns to activity prior fully healing, increasing a predisposition for additional chronic pain. Monitoring healing progression after injury involves different types of imaging but these approaches suffer from several disadvantages. Isolating profiling transcripts injured site would abrogate shortcomings provide enumerative insights into regenerative potential...