A5100391248- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- DNA Repair Mechanisms
- Genomics and Phylogenetic Studies
- Hemoglobinopathies and Related Disorders
- Molecular Biology Techniques and Applications
- Chromosomal and Genetic Variations
- RNA Interference and Gene Delivery
- Genetic Neurodegenerative Diseases
- Congenital heart defects research
- HIV Research and Treatment
- Biomedical Ethics and Regulation
- Vibrio bacteria research studies
- Protein Degradation and Inhibitors
- Genomics and Chromatin Dynamics
- Genomic variations and chromosomal abnormalities
- CAR-T cell therapy research
- Insect and Arachnid Ecology and Behavior
- Cardiac Fibrosis and Remodeling
- Plant and Fungal Species Descriptions
- Folate and B Vitamins Research
- Microbial Natural Products and Biosynthesis
- Plant Reproductive Biology
- Biochemical and Molecular Research
- Metabolism and Genetic Disorders
Huizhou University
2024
Gladstone Institutes
2019-2024
Guiyang University
2022
University of California, San Francisco
2006-2021
Yale University
2007
Osaka University
1993-1996
Individuals homozygous for the C-C chemokine receptor type 5 gene with 32-bp deletions (CCR5Δ32) are resistant to HIV-1 infection. In this study, we generated induced pluripotent stem cells (iPSCs) naturally occurring CCR5Δ32 mutation through genome editing of wild-type iPSCs using a combination transcription activator-like effector nucleases (TALENs) or RNA-guided clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 together piggyBac technology. Remarkably, TALENs...
The innovation of reprogramming somatic cells to induced pluripotent stem provides a possible new approach treat β-thalassemia and other genetic diseases such as sickle cell anemia. Induced (iPS) can be made from these patients' the mutation in β-globin gene corrected by targeting, differentiated into hematopoietic returned patient. In this study, we reprogrammed skin fibroblasts patient with homozygous β 0 thalassemia iPS cells, showed that could synthesized hemoglobin. Prenatal diagnosis...
Derivation of human hematopoietic stem cells (HSCs) from induced pluripotent (iPSCs) offers considerable promise for cell therapy, disease modeling, and drug screening. However, efficient derivation functional iPSC-derived HSCs with in vivo engraftability multilineage potential remains challenging. Here, we demonstrate a tractable approach respecifying blood into highly engraftable progenitor (HSPCs) through transient expression single transcription factor, MLL-AF4 These HSPCs (iHSPCs)...
The discovery of induced pluripotent stem cells (iPSCs) holds great promise for regenerative medicine since it is possible to produce patient-specific from affected individuals potential autologous treatment. Using nonintegrating cytoplasmic Sendai viral vectors, we generated iPSCs efficiently adult mobilized CD34⁺ and peripheral blood mononuclear cells. After 5-8 passages, the genome could not be detected by real-time quantitative reverse transcription-polymerase chain reaction. spin...
Cystic fibrosis (CF) is a recessive inherited disease associated with multiorgan damage that compromises epithelial and inflammatory cell function. Induced pluripotent stem cells (iPSCs) have significantly advanced the potential of developing personalized cell-based therapy for diseases like CF by generating patient-specific can be differentiated into repair tissues damaged pathology. The F508del mutation in airway cell-derived CF-iPSCs was corrected small/short DNA fragments (SDFs)...
To date, a large number of reports have described reprogramming many somatic cell types into induced pluripotent stem (iPS) cells, using different numbers transcription factors and devising alternate methods introducing the factor genes or proteins cells. Here, we describe method bacteriophage ΦC31 integrase to reprogram mouse embryonic fibroblasts human amniotic fluid cells iPS These showed morphology, surface antigens, gene expression, epigenetic states similar ES formed teratomas with...
Sickle cell anemia is one of the most common genetic diseases worldwide. Patients often suffer from anemia, painful crises, infections, strokes, and cardiopulmonary complications. Although current management has improved quality life survival patients, cure can be achieved only with bone marrow transplantation when histocompatible donors are available. The ES technology suggests that a therapeutic cloning approach may feasible for treatment this disease. Using transgenic/knockout sickle...
DNA transposon piggyBac (PB) is a newly established mutagen for large-scale mutagenesis in mice. We have designed and implemented an integrated database system called PBmice (PB Mutagenesis Information CEnter) storing, retrieving displaying the information derived from PB insertions (INSERTs) mouse genome. This centered on INSERTs with including their genomic locations flanking sequences, expression levels of hit genes, patterns trapped genes if trapping vector was used. It also archives...
Outbreaks of bacterial diseases occur in farmed Chinese longsnout catfish (Leiocassis longirostris). Due to limited information on aquatic Klebsiella variicola-infected animals, this study aimed identify strain LL2208 isolated from diseased L. longirostris, determine its biological features, and evaluate risk public health. Strain was tested for molecular identification, challenge, string, biofilm formation, antimicrobial susceptibility. Furthermore, the whole genome sequenced analyzed....
Ectopic overexpression of transcription factors has been used to reprogram cell fate. For example, virus-mediated four OCT4, SOX2, MYC, and KLF4, known as Yamanaka factors, can convert somatic cells induced pluripotent stem (iPS) cells. However, gene-specific switch-on endogenous gene production without the use foreign DNA remains a challenge. The small RNA machinery that comprised RNAs Argonaute proteins is silence expression, but be repurposed activate expression when directed promoters,...
Summary Hereditary persistence of fetal haemoglobin (HPFH) is the major modifier clinical severity β‐thalassaemia. The homozygous mutation c.‐196 C>T in Aγ‐globin ( HBG1 ) promoter, which causes Sardinian δβ 0 ‐thalassaemia, able to completely rescue β‐major thalassaemia phenotype caused by β 39‐thalassaemia mutation, ensuring high levels synthesis during adulthood. Here, we describe a CRISPR/Cas9 genome‐editing approach, combined with non‐homologous end joining (NHEJ) pathway repair,...
Oligo/polynucleotide-based gene targeting strategies provide new options for achieving sequence-specific modification of genomic DNA and have implications the development therapies transgenic animal models. One such strategy, small fragment homologous replacement (SFHR), was evaluated qualitatively quantitatively in human lymphoblasts that contain a single base substitution hypoxanthine-guanine phosphoribosyl transferase (HPRT1) gene. Because HPRT1 mutant cells are readily discernable from...
The genus Galerita Fabricius, 1801 belongs to the tribe Galeritini of family Carabidae. In this study, complete mitochondrial genome (GenBank: ON920164.1) G. orientalis is newly sequenced, annotated, characterized, and composed 37 typical genes, one control region. Mitogenome a circular DNA molecule 16,137 bp with 78.79% AT content. All 13 protein-coding genes are initiated using ATN (Met) as start codon, except for nad1, which has TTG terminated TAN stop codon. Twenty-two tRNAs could fold...
Fibroblasts can be transdifferentiated directly into other somatic cells such as cardiomyocytes, hematopoietic cells, and neurons. An advantage of cell differentiation without first generating induced pluripotent stem (iPSCs) is that it avoids contamination the differentiated with residual iPSCs, which may cause teratoma. However, since primary fibroblasts from biopsy undergo senescence during repeated culture, difficult to grow in sufficient numbers for future therapeutic purposes. To...
Objectives: The discovery of induced pluripotent stem cells (iPSC) has brought promise to regenerative medicine as it breaks the ethical barrier using embryonic cells. Such cell culture-derived patient-specific autologous are needed for transplantation. Here we report deriving HIV-1-infected patients’ iPSC lines under transgene-free methods and feeder-free xeno-free culture conditions meet requirement clinical application. Methods results: We have reprogrammed peripheral blood mononuclear...
Genetic editing of induced pluripotent stem (iPS) cells represents a promising avenue for an HIV cure. However, certain challenges remain before bringing this approach to the clinic. Among them, in vivo engraftment genetically edited vitro needs be achieved. In study, CD34
Camellia nitidissima Chi (CNC), a species of golden Camellia, is well known as “the queen camellias.” It an ornamental, medicinal, and edible plant grown in China. In this study, we conducted genome survey sequencing analysis simple sequence repeat (SSR) identification CNC using the Illumina platform. The 21-mer predicted its size to be 2,778.82 Mb, with heterozygosity repetition rates 1.42% 65.27%, respectively. sequences were assembled into 9,399,197 scaffolds, covering ∼2,910 Mb N50 869...
Journal Article Population variation in the dinucleotide repeat polymorphism at D8S360 locus Get access Kouzin Kamino, Kamino * *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Jun Nakura, Nakura Koichi Kihara, Kihara Lin Ye, Ye Keiko Nagano, Nagano Tohru Ohta, Ohta 1Department of Human Genetics, Nagasaki University School MedicineSakamoto-Machi 1-12-4, 852, Japan Yoshihiro Jinno, Jinno Norio Niikawa, Niikawa Tetsuro...