A5086792381- Pancreatic function and diabetes
- Receptor Mechanisms and Signaling
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism, Diabetes, and Cancer
- Diabetes Treatment and Management
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA modifications and cancer
- Neuropeptides and Animal Physiology
- Growth Hormone and Insulin-like Growth Factors
- Diabetes and associated disorders
- Liver Disease Diagnosis and Treatment
- TGF-β signaling in diseases
- Mechanisms of cancer metastasis
- Regulation of Appetite and Obesity
- Diet, Metabolism, and Disease
- Genetic Neurodegenerative Diseases
- Ferroptosis and cancer prognosis
- Pancreatic and Hepatic Oncology Research
- Liver physiology and pathology
- Neuroscience and Neuropharmacology Research
Nantong University
2018-2025
Tianjin Medical University
2022-2024
Tianjin Medical University General Hospital
2024
Hangzhou Cancer Hospital
2023
Zhejiang University
2023
National Institute of Diabetes and Digestive and Kidney Diseases
2015-2022
Hubei University of Chinese Medicine
2022
Chinese Academy of Sciences
2007-2020
National Institutes of Health
2015-2020
University of South China
2019-2020
Glucagon, a hormone released from pancreatic α cells, plays key role in maintaining proper glucose homeostasis and has been implicated the pathophysiology of diabetes. In vitro studies suggest that intraislet glucagon can modulate function β cells. However, because lack suitable experimental tools, vivo physiological this cross-talk remained elusive. To address issue, we generated mouse model selectively expressed an inhibitory designer GPCR (Gi DREADD) cells only. Drug-induced activation...
Upon activation, TGF-β type I receptor (TβRI) undergoes active ubiquitination via recruitment of E3 ligases to the complex by Smad7. However, how TβRI is coupled intracellular trafficking, and protein degradation remains unclear. We report here that Tollip, an adaptor contains both ubiquitin-associated domains endosome-targeting domain, plays important role in modulating trafficking TβRI. Tollip was previously demonstrated possess a functional signaling interleukin-1 Toll-like receptors....
β-arrestins are critical signalling molecules that regulate many fundamental physiological functions including the maintenance of euglycemia and peripheral insulin sensitivity. Here we show inactivation β-arrestin-2 gene, barr2, in β-cells adult mice greatly impairs release glucose tolerance fed with a calorie-rich diet. Both KCl-induced secretion calcium responses were profoundly reduced (barr2) deficient β-cells. In human β-cells, barr2 knockdown abolished glucose-induced secretion. We...
Abstract The mitochondrial enriched GCN5-like 1 (GCN5L1) protein has been shown to modulate acetylation, content and retrograde signaling. Here we show that hepatic GCN5L1 ablation reduces fasting glucose levels blunts gluconeogenesis without affecting systemic tolerance. PEPCK G6Pase transcript are downregulated in hepatocytes from liver specific knockout mice their upstream regulator, FoxO1 decreased via proteasome-dependent degradation reactive oxygen species mediated ERK-1/2...
Abstract Hydroxytyrosol (HTS) is a natural polyphenol abundant in olive oil. Increasing evidence indicates HTS has beneficial effect on human health for preventing various diseases. In the present study, we investigated protective effects of acrolein‐induced toxicity retinal pigment epithelial cell line, ARPE‐19, cellular model smoking‐ and age‐related macular degeneration. Acrolein, major component gas phase cigarette smoke also product lipid peroxidation vivo , at 75 μmol/L 24 h caused...
Abstract β-Arrestins are major regulators of G protein-coupled receptor-mediated signaling processes. Their potential roles in regulating adipocyte function vivo remain unexplored. Here we report the novel finding that mice lacking β-arrestin-2 (barr2) selectively adipocytes show significantly reduced adiposity and striking metabolic improvements when consuming excess calories. We demonstrate these beneficial effects due to enhanced through β3-adrenergic receptors (β3-ARs), indicating barr2...
To solve the complexity of traditional motion intention recognition method using a multi-mode sensor signal and lag process, in this paper, an inertial sensor-based for soft exoskeleton is proposed. Compared with recognition, addition to classic five kinds terrain, transformed terrain also added. In mode acquisition, sensors’ data thigh calf different modes are collected. After series preprocessing, such as filtering normalization, sliding window used enhance data, so that each frame...
Most animals experience fasting-feeding cycles throughout their lives. It is well known that the liver plays a central role in regulating glycogen metabolism. However, how hepatic glycogenesis coordinated with cycle to control postprandial glucose homeostasis remains largely unknown. This study determines molecular mechanism underlying coupling of cycle.Through series molecular, cellular, and animal studies, we investigated PPP1R3G, glycogen-targeting regulatory subunit protein phosphatase 1...
Phosphoinositide 3-kinase (PI3K) mediates insulin actions by relaying signals from receptors (IRs) to downstream targets. The p110α catalytic subunit of class IA PI3K is the primary insulin-responsive implicated in signaling. We demonstrate here a new mode spatial regulation for PAQR3 that exclusively localized Golgi apparatus. interacts with p110α, and intracellular targeting apparatus reduced downregulation increased overexpression. Insulin-stimulated activity phosphoinositide...
PAQR3 is a member of the progestin and adipoQ receptor (PAQR) family was recently characterized as spatial regulator that negatively modulates Ras/Raf/MEK/ERK signaling cascade. However, little known about physiological functions in tumorigenesis colorectal cancers. The function cancer development mice analyzed by crossing Paqr3-depleted with Apc Min/+ have germline mutation gene-encoding tumor suppressor adenomatous polyposis coli (APC). survival time area small intestine significantly...
An increase in hepatic glucose production (HGP) represents a key feature of type 2 diabetes. This deficiency metabolic control critically depends on enhanced signaling through glucagon receptors (GCGRs). Here, we have demonstrated that selective inactivation the GPCR-associated protein β-arrestin hepatocytes adult mice results greatly increased GCGR signaling, leading to striking deficits homeostasis. However, hepatocyte-specific did not affect insulin sensitivity or β-adrenergic signaling....
An increase in hepatic glucose production (HGP) is a key feature of type 2 diabetes. Excessive signaling through Gs-linked glucagon receptors critically contributes to pathologically elevated HGP. Here, we tested the hypothesis that this metabolic impairment can be counteracted by enhancing Gi signaling. Specifically, used chemogenetic approach selectively activate Gi-type G proteins mouse hepatocytes vivo. Unexpectedly, activation triggered pronounced HGP and severely impaired homeostasis....
Abstract Adipocyte dysfunction links obesity to insulin resistance and type 2 diabetes. function is regulated by receptor-mediated activation of heterotrimeric G proteins. Little known about the potential in vivo metabolic roles i -type proteins expressed adipocytes, primarily due lack suitable animal models. To address this question, we generated mice lacking functional selectively adipocytes. Here report that these mutant displayed significantly impaired glucose tolerance reduced...
Abstract Background Glutaminolysis is a critical metabolic process that promotes cancer cell proliferation, including hepatocellular carcinoma (HCC). Delineating the molecular control of glutaminolysis could identify novel targets to ameliorate this oncogenic pathway. Here, we evaluated role general amino acid synthesis 5 like 1 (GCN5L1), regulator mitochondrial protein acetylation, in modulating acetylation and activity glutaminase regulate HCC development. Methods Cell proliferation was...
Diet-induced obesity is commonly associated with leptin resistance, and attenuated signaling contributes to the progression of obesity. PAQR3 a member progesterone AdipoQ receptor (PAQR) family close homology adiponectin receptors. We hypothesized that implicated in regulation energy homeostasis. To address this hypothesis, we fed Paqr3-deleted mice high-fat diet (HFD), followed by analyses evaluate obesity, hepatic steatosis, insulin metabolic rate, signaling. found deletion Paqr3 are...
Liver glycogen metabolism plays an important role in glucose homeostasis. Glycogen synthesis is mainly regulated by synthase that dephosphorylated and activated protein phosphatase 1 (PP1) combination with glycogen-targeting subunits or G subunits. There are seven (PPP1R3A to G) control glycogenesis different organs. PPP1R3G a recently discovered subunit whose expression changed along the fasting-feeding cycle proposed play postprandial In this study, we analyzed physiological function of...
Abstract Insulin-like growth factor 1 (IGF1) is a key for tissue and fuel metabolism. The potential function of central IGF1 remains unclear. We previously observed that expression increased in the hypothalamus obese mice lacking STAT5 nervous system (CNS). In this study, we explored metabolic by intracerebroventricular (ICV) injection IGF1, over-expression administering an adeno-associated virus (AAV), ICV anti-IGF1 antibody. Mice over-expressed displayed appetite, improved glucose...