A5008090688- Pluripotent Stem Cells Research
- Aquaculture disease management and microbiota
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- Privacy-Preserving Technologies in Data
- Congenital heart defects research
- Vibrio bacteria research studies
- MicroRNA in disease regulation
- Fetal and Pediatric Neurological Disorders
- Chromosomal and Genetic Variations
- Genetic and Kidney Cyst Diseases
- Hedgehog Signaling Pathway Studies
- Structural Engineering and Vibration Analysis
- Antibiotic Resistance in Bacteria
- Salmonella and Campylobacter epidemiology
- Stochastic Gradient Optimization Techniques
- Image and Signal Denoising Methods
- Protist diversity and phylogeny
- Microbial infections and disease research
- Neonatal Respiratory Health Research
- Machine Learning and ELM
University of California, Berkeley
2022-2025
Zhejiang University of Technology
2023-2024
Shanghai Jiao Tong University
2009-2024
Nanjing University of Information Science and Technology
2024
Songjiang District Central Hospital
2024
University of British Columbia
2022
Dalian Ocean University
2018-2019
Newcastle University
2016
Shanghai Institutes for Biological Sciences
2009-2011
Chinese Academy of Sciences
2009-2011
Direct reprogramming of human somatic cells into pluripotency has broad implications in generating patient-specific induced pluripotent stem (iPS) for disease modeling and cellular replacement therapies. However, the low efficiency safety issues associated with generation iPS have limited their usage clinical settings. Cell types can significantly influence kinetics. To date, been obtained only from a few cell types. Here, we report first time rapid efficient amniotic fluid-derived (hAFDCs)...
Choroid plexus is a major site for cerebrospinal fluid (CSF) production, characterized by multiciliated epithelial monolayer that regulates CSF production. We demonstrate defective choroid ciliogenesis or Intraflagellar transport yields neonatal hydrocephalus, at least in part, due to increased water channel Aqp1 and ion transporter Atp1a2 expression. multicilia as sensory cilia, transducing both canonical non-canonical Shh signaling. Interestingly, it the signaling represses expression...
Highlights•Choroid plexus cilia defects contribute to a neonatal hydrocephalus phenotype•Choroid exhibit distinct ultrastructure resembling primary and motile cilia•Choroid transduce Shh repress water channel ion transporter expression•Developmental dynamics of choroid ciliary length governs the signaling intensitySummaryThe is major site for cerebrospinal fluid (CSF) production, characterized by multiciliated epithelial monolayer that regulates CSF production. We demonstrate defective...
Human induced pluripotent stem cell (hiPSC) utility is limited by variations in the ability of these cells to undergo lineage-specific differentiation. We have undertaken a transcriptional comparison human embryonic (hESC) lines and hiPSC shown that hiPSCs are inferior their neuroectodermal Among differentially expressed candidates between hESCs hiPSCs, we identified mitochondrial protein, CHCHD2, whose expression seems correlate with differentiation potential cells. provide evidence...
GATA4 (GATA-binding protein 4), a zinc finger-containing, DNA-binding transcription factor, is essential for normal cardiac development and homeostasis in mice humans, mutations this gene have been reported human heart defects. Defects alternative splicing are associated with many diseases, yet relatively little known about how cell type- or state-specific achieved the heart. Here, we show that regulates type-specific through direct interaction RNA spliceosome induced pluripotent stem...
Human embryonic stem cells (hESCs) and human induced pluripotent (hiPSCs) are defined as in view of their self-renewal ability potential to differentiate all three germ layers. Recent studies have indicated that microRNAs (miRNAs) play an important role the maintenance pluripotency cell cycle regulation. We used a microarray based approach identify miRNAs were enriched hESCs when compared differentiated at same time showed significant expression changes between different phases cycle....
Abstract Biogenesis of organelles requires targeting a subset proteins to specific subcellular domains by signal peptides or mechanisms controlling mRNA localization and local translation. How distribution translation mRNAs for organelle biogenesis is achieved remains elusive likely be dependent on the cellular context. Here we identify Trinucleotide repeat containing-6a ( Tnrc6a ), component miRNA pathway, distinctively localized apical granules differentiating airway multiciliated cells...
Induced pluripotent stem (iPS) cells have attracted enormous attention due to their vast potential in regenerative medicine, pharmaceutical screening and basic research. Most prior established iPS cell lines were derived maintained on mouse embryonic fibroblast (MEF) supplemented with exogenous leukemia inhibitory factor (LIF). Drawbacks of MEF impede optimization as well dissection reprogramming events limit the usage derivatives therapeutic applications. In this study, we develop a...
In this study, bacterial pathogens were isolated from the liver, spleen, kidney, heart, and muscle of diseased goldfish (Carassius auratus) after a massive outbreak disease in an aquaculture farm Anshan, Liaoning Province, North China (N41°05′58.42″ E122°53′31.89″). Based on physiological biochemical characterization bacteria 16S rDNA sequence analysis, isolates identified as Aeromonas salmonicida salmonicida. Virulence gene multilocus typing analysis showed that all organs collected same...
Non-O1/O139 Vibrio cholerae is increasingly reported in the clinical settings. However, intestinal infections via consumption of non-O1/O139 V. cholerae-carrying seafood are rarely documented China. In this study, we a case mild watery diarrhea young male, caused by downstream Liaohe River. Epidemiological investigation showed that infection potentially associated with raw mollusc. Prior to finding, conducted 6-month pathogen surveillance three locations along River and identified...
To evaluate the overuse of antibiotics and to identify origin pathogens in ornamental fish industry, we conducted a field investigation three representative farms Liaoning province, China. Drug-resistant fishponds groundwater were isolated subtyped by multilocus sequence typing (MLST). In total, 33 pathogenic strains, including Aeromonasveronii five other pathogens, from diseased groundwater. MLST revealed that A.veronii obtained can be into four types, which also identified corresponding...
Previously, a pathogenic ciliate was isolated from the surface ulcer of diseased Turbot (Scophthalmus maximus L.) at an aquaculture farm in North China. After morphological and molecular biological identification based on 18rRNA, ciliated identified as notorious scuticociliates (Pseudocohnilembus persalinus). In this study, whole sequence mitochondrial genomic gene P. persalinus carried out. The sequencing results showed that complete mitogenome 38,375 bp. There were 2 rRNAs, 4 tRNAs, 34...
L-amino acid oxidase (LAAO) is able to stereoselectively catalyse the deamination of acids α-keto acids, ammonia and H2O2. It has been reported that this enzyme biological functions including inhibition bacteria antiviral, anti-parasitic anti-tumour activities. To date, marine animal LAAO are constantly being identified. derived from animals purified for Sebastes schlegelii, Platichthys stellatus, Gadus morhua, Signanus oramin, Aplysia californica some others. In paper, we review progress...
Over the past few years, federated learning has become widely used in various classical machine fields because of its collaborative ability to train data from multiple sources without compromising privacy. However, area graph neural networks, nodes and network structures graphs held by clients are different many practical applications, aggregation method that directly shares model gradients cannot be applied this scenario. Therefore, work proposes a FLGNN federation scenarios investigates...
The human retina, a complex and highly specialized structure, includes multiple cell types that work synergistically to generate transmit visual signals. However, genetic predisposition or age-related degeneration can lead retinal damage severely impairs vision causes blindness. Treatment options for diseases are limited, there is an urgent need innovative therapeutic strategies. Cell gene therapies promising because of the efficacy delivery systems transport genes targeted cells. Gene hold...
Abstract Biogenesis of organelles requires targeting a subset proteins to specific subcellular domains by signal peptides or mechanisms controlling mRNA localization and local translation. How distribution translation mRNAs for organelle biogenesis is achieved remains elusive likely be dependent on the cellular context. Here we identify Trinucleotide repeat containing-6a ( Tnrc6a) , component miRNA pathway, distinctively localized apical granules differentiating airway multiciliated cells...
Biogenesis of organelles requires targeting subset proteins to specific subcellular domains by signal peptides or mechanisms controlling mRNA localization and local translation. How distribution translation mRNAs for organelle biogenesis is achieved remains elusive likely be dependent on the cellular context. Here we identify Trinucleotide repeat containing-6a (Tnrc6a), a component miRNA pathway, distinctively localized apical granules differentiating airway multiciliated cells (MCCs)...
Real-world systems are dynamic and complex. Networks can be used to represent these real-world systems. Cluster formation is very important challenging problems regarding the networks, especially since networks hard distinguish communities. DynaMo, which community detection by incrementally maximizing modularity analysis, one of algorithms that cluster networks. In this paper, principle methodology DynaMo discussed introduce basic background DynaMo. order test performance an experiment...