Luisa Imberti
A5056910421- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Immunotherapy and Immune Responses
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Cytomegalovirus and herpesvirus research
- Multiple Sclerosis Research Studies
- CAR-T cell therapy research
- Hematopoietic Stem Cell Transplantation
- HIV Research and Treatment
- Polyomavirus and related diseases
- Cancer Immunotherapy and Biomarkers
- Chronic Lymphocytic Leukemia Research
- COVID-19 and healthcare impacts
- Diabetes and associated disorders
- Systemic Lupus Erythematosus Research
- vaccines and immunoinformatics approaches
- Viral Infections and Immunology Research
- Blood disorders and treatments
- Cutaneous Melanoma Detection and Management
- Full-Duplex Wireless Communications
- Parvovirus B19 Infection Studies
- Long-Term Effects of COVID-19
University of Brescia
2010-2025
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
2015-2025
Research Institute Hospital 12 de Octubre
2024
University of South Florida
2022
Flinders Medical Centre
2022
Flinders University
2022
New York Medical College
1985-2015
Institute of Cell Biology and Neurobiology
2013
Inserm
2013
University of Münster
2013
Interindividual clinical variability in the course of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is vast. We report that at least 101 987 patients with life-threatening disease 2019 (COVID-19) pneumonia had neutralizing immunoglobulin G (IgG) autoantibodies (auto-Abs) against interferon-ω (IFN-ω) (13 patients), 13 types IFN-α (36), or both (52) onset critical disease; a few also auto-Abs other three type I IFNs. The neutralize ability corresponding IFNs to block...
The genetics underlying severe COVID-19 immune system is complex and involves many genes, including those that encode cytokines known as interferons (IFNs). Individuals lack specific IFNs can be more susceptible to infectious diseases. Furthermore, the autoantibody dampens IFN response prevent damage from pathogen-induced inflammation. Two studies now examine likelihood affects risk of coronavirus disease 2019 (COVID-19) through components this (see Perspective by Beck Aksentijevich). Q....
Circulating autoantibodies (auto-Abs) neutralizing high concentrations (10 ng/mL, in plasma diluted 1 to 10) of IFN-α and/or -ω are found about 10% patients with critical COVID-19 pneumonia, but not subjects asymptomatic infections. We detect auto-Abs 100-fold lower, more physiological, (100 pg/mL, 1/10 dilutions plasma) 13.6% 3,595 COVID-19, including 21% 374 > 80 years, and 6.5% 522 severe COVID-19. These antibodies also detected 18% the 1,124 deceased (aged 20 days-99 years; mean: 70...
Immune and inflammatory responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contribute disease severity of 2019 (COVID-19). However, the utility specific immune-based biomarkers predict clinical outcome remains elusive. Here, we analyzed levels 66 soluble in 175 Italian patients with COVID-19 ranging from mild/moderate critical assessed type I IFN–, II NF-κB–dependent whole-blood transcriptional signatures. A broad signature was observed, implicating activation various...
T cells are involved in the early identification and clearance of viral infections also support development antibodies by B cells. This central role for makes them a desirable target assessing immune response to SARS-CoV-2 infection. Here, we combined two high-throughput profiling methods create quantitative picture T-cell SARS-CoV-2. First, at individual level, deeply characterized 3 acutely infected 58 recovered COVID-19 subjects experimentally mapping their CD8 through antigen stimulation...
Significance There is growing evidence that preexisting autoantibodies neutralizing type I interferons (IFNs) are strong determinants of life-threatening COVID-19 pneumonia. It important to estimate their quantitative impact on mortality upon SARS-CoV-2 infection, by age and sex, as both the prevalence these risk death increase with higher in men. Using an unvaccinated sample 1,261 deceased patients 34,159 individuals from general population, we found against IFNs strongly increased...
Patients with autoimmune polyendocrinopathy syndrome type 1 (APS-1) caused by autosomal recessive AIRE deficiency produce autoantibodies that neutralize I interferons (IFNs)1,2, conferring a predisposition to life-threatening COVID-19 pneumonia3. Here we report patients NIK or RELB deficiency, specific of autosomal-dominant NF-κB2 also have neutralizing against IFNs and are at higher risk getting pneumonia. In these found only in individuals who heterozygous for variants associated both...
Immune system aging is becoming a field of increasing public health interest because prolonged life expectancy, which not paralleled by an increase in expectancy. As age progresses, innate and adaptive immune systems undergo changes, are defined, respectively, as inflammaging senescence. A wealth available data demonstrates that these two conditions closely linked, leading to greater vulnerability elderly subjects viral, bacterial, opportunistic infections well lower post-vaccination...
The mechanism of T cell depletion during infection with the human immunodeficiency virus (HIV) is unclear. Examination repertoire receptor V (variable) regions in persons infected HIV revealed absence a common set beta regions, whereas alpha usage was normal. lack these segments did not appear to correlate opportunistic infections. selective elimination cells that express defined sequences may indicate presence an HIV-encoded superantigen, similar those encoded by long terminal repeat mouse...
Abstract Synovial fluids of patients with rheumatoid arthritis contain activated T lymphocytes that may play an important role in the pathogenesis disease. Previous studies have suggested cell receptor (TcR) repertoire these cells is restricted, reflecting vivo selection a limited number specificities at site inflammation. To characterize better populations we used polymerase chain reaction technology to estimate proportion TcR α and β RNA containing any particular V elements from...
Altered glomerular metabolism of arachidonic acid (AA) has already been demonstrated in experimental nephrotoxic nephritis. The enhanced synthesis thromboxane A2 (TxA2) isolated glomeruli that found may mediate changes renal hemodynamics. objectives this investigation were: to check whether AA is also altered a model glomerulopathy which no leukocyte infiltration or platelet deposition could be demonstrated; establish correlation between the and proteinuria; explore alteration prostaglandin...
To find biomarkers identifying patients at risk for the development of progressive multifocal leukoencephalopathy (PML) during natalizumab treatment.Patients were recruited from 10 European and US cohorts. Of 289 with multiple sclerosis (MS), 224 had been treated (18-80 months), 21 received other immune-modulatory treatments, 28 untreated. We access to samples 16 PML patients. Eight these given blood before diagnosis PML. also analyzed non-natalizumab-treated who developed (n = 10) age-...
SARS-CoV-2 vaccination is recommended in patients with inborn errors of immunity (IEIs); however, little known about immunogenicity and safety these patients.
Type-I interferons (IFNs) mediate antiviral activity and have emerged as important immune mediators during coronavirus disease 19 (COVID-19). Several lines of evidence suggest that impaired type-I IFN signaling may predispose to severe COVID-19. However, the pathophysiologic mechanisms contribute illness severity remain unclear. In this study, our goal was gain insight into how IFNs influence outcomes in patients with To achieve goal, we compared clinical between 26 neutralizing...
The thymus plays a fundamental role in establishing and maintaining central peripheral tolerance defects thymic architecture or AIRE expression result the development of autoreactive lymphocytes. Patients with partial DiGeorge Syndrome (DGS) Down (DS) present alterations size higher risk to develop autoimmunity. We sought evaluate thymocyte DGS DS patients determine extent which immune dysregulation T cell homeostasis perturbation these patients. Thymi from pediatric age-matched controls...
Life-threatening “breakthrough” cases of critical COVID-19 are attributed to poor or waning antibody (Ab) response SARS-CoV-2 vaccines in individuals already at risk. Preexisting auto-Abs neutralizing type I IFNs underlie least 15% pneumonia unvaccinated individuals; their contribution hypoxemic breakthrough vaccinated people is unknown. We studied a cohort 48 (aged 20 86 years) who received two doses messenger RNA (mRNA) vaccine and developed infection with 2 weeks 4 months later. Ab levels...
Abstract Combination of chemotherapy with cancer vaccines is currently regarded as a potentially valuable therapeutic approach for the treatment some metastatic tumors, but optimal modalities remain unknown. We designed phase I/II pilot study evaluating effects dacarbazine (DTIC) on immune response in HLA‐A2 + disease‐free melanoma patients who received anticancer vaccination 1 day following (800 mg/mq i.v.). The vaccine, consisting combination restricted antigen A (Melan‐A/MART‐1) and gp100...