A5025265080- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Cytokine Signaling Pathways and Interactions
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- interferon and immune responses
- CAR-T cell therapy research
- Polyomavirus and related diseases
- Immune cells in cancer
- Cytomegalovirus and herpesvirus research
- COVID-19 Clinical Research Studies
- Influenza Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Adenosine and Purinergic Signaling
- thermodynamics and calorimetric analyses
- Gut microbiota and health
- Mesenchymal stem cell research
- Barrier Structure and Function Studies
- vaccines and immunoinformatics approaches
- Phagocytosis and Immune Regulation
- Cancer-related gene regulation
- Immune responses and vaccinations
- Histone Deacetylase Inhibitors Research
Istituto Superiore di Sanità
2013-2025
Georgia State University
2015
Molecular Oncology (United States)
2007-2014
University Hospital of Basel
2005-2009
University of Basel
2006-2007
Successful chemotherapy accounts for both tumor-related factors and host immune response. Compelling evidence suggests that some chemotherapeutic agents can induce an immunogenic type of cell death stimulating tumor-specific immunity. Here, we show cyclophosphamide (CTX) exerts two types actions relevant the induction antitumor immunity in vivo: (i) effect on dendritic (DC) homeostasis, mediated by endogenous I interferons (IFN-I), leading to preferential expansion CD8α(+) DC, main subset...
Cancer stem cells (CSCs) are a subpopulation of cancer endowed with high tumorigenic, chemoresistant and metastatic potential. Nongenetic mechanisms acquired resistance increasingly being discovered, but molecular insights into the evolutionary process CSCs limited. Here, we show that type I interferons (IFNs-I) function as hubs during immunogenic chemotherapy, triggering epigenetic regulator demethylase 1B (KDM1B) to promote an adaptive, yet reversible, transcriptional rewiring towards...
Abstract Purpose: Immunotherapy is a promising antitumor strategy, which can be successfully combined with current anticancer treatments, as suggested by recent studies showing the paradoxical chemotherapy-induced enhancement of immune response. The purpose present work to dissect biological events induced chemotherapy that cooperate immunotherapy in success treatment against cancer. In particular, we focused on following: (a) cyclophosphamide-induced modulation several cytokines, (b)...
Abstract The identification of natural adjuvants capable selectively promoting an efficient immune response against infectious agents would represent important advance in immunology, with direct implications for vaccine development, whose progress is generally hampered by the difficulties defining powerful synthetic suitable clinical use. Here, we demonstrate that endogenous type I IFN necessary Th1 induced typical mice and itself unexpectedly adjuvant when administered human influenza...
To investigate the ability of bone marrow (BM)-derived mesenchymal stromal cells (BM-MSCs) in suppressing proliferation stimulated lymphocytes across a range conditions including autologous BM-MSCs derived from autoimmune disease (AD) patients.In vitro cultures healthy donors and AD patients were established characterized by their differentiation potential into adipocytes osteoblasts, fibroblast-colony-forming unit (CFU-F) phenotype flow cytometry. (irradiated non-irradiated) tested for to...
Cross-presentation is a crucial mechanism for generating CD8 T cell responses against exogenous Ags, such as dead cell-derived Ag, and mainly fulfilled by CD8α(+) dendritic cells (DC). Apoptotic death occurring in steady-state conditions largely tolerogenic, thus hampering the onset of effector responses. Type I IFNs (IFN-I) have been shown to promote cross-priming soluble or viral partly through stimulation DC. By using UV-irradiated OVA-expressing mouse EG7 thymoma cells, we show that...
Abstract Combination of chemotherapy with cancer vaccines is currently regarded as a potentially valuable therapeutic approach for the treatment some metastatic tumors, but optimal modalities remain unknown. We designed phase I/II pilot study evaluating effects dacarbazine (DTIC) on immune response in HLA‐A2 + disease‐free melanoma patients who received anticancer vaccination 1 day following (800 mg/mq i.v.). The vaccine, consisting combination restricted antigen A (Melan‐A/MART‐1) and gp100...
Abstract Background Bacterial toxins are emerging as promising hallmarks of colorectal cancer (CRC) pathogenesis. In particular, Cytotoxic Necrotizing Factor 1 (CNF1) from E. coli deserves special consideration due to the significantly higher prevalence this toxin gene in CRC patients with respect healthy subjects, and numerous tumor-promoting effects that have been ascribed vitro. Despite evidence, a definitive causal link between CNF1 was missing. Here we investigated whether plays an...
AuNP and AuNP/DXM stability drug release kinetics in different biological media.
<h3>Objectives:</h3> To investigate whether human bone marrow-derived mesenchymal stem cells (BM-MSCs) and articular chondrocytes (ACs) affect the in vitro proliferation of T lymphocytes peripheral blood mononuclear (PBMCs) driven by homeostatic interleukin (IL)2, IL7 IL15 cytokines binding to common cytokine receptor γ-chain (γ<sub>c</sub>) absence cell (TCR) triggering. <h3>Methods:</h3> PBMCs, total subsets (CD4+ CD8+) were stimulated with IL2, or exposed cultured BM-MSCs ACs at varying...
Abstract DC are the most potent antigen‐presenting cells that recognise signs of infection and serve as main activators naïve T cells. We have previously shown type I IFN (IFN‐I) produced by can act in an autocrine manner to activate DC. In present study, we investigated role IFN‐I regulating turnover lifespan found DC, especially CD8α + subset, from receptor knock out (IFNAR KO) mice, display a reduced rate when compared with WT revealed BrdU labelling kinetics. vitro , IFNAR KO BM...
Human polyomavirus BK (BKV) has been implicated in oncogenic transformation. Its ability to replicate is determined by the binding of its large tumor antigen (LTag) products tumor-suppressor genes regulating cell cycle, as specifically p53. We investigated CD8+ T immune responses BKV LTag portions involved p53 HLA-A*0201+ experienced individuals. Peptides selected from either p53-binding region (LTag351–450 and LTag533–626) current algorithms capacity bind HLA-A*0201 molecule were used...