A5086803537- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- COVID-19 and healthcare impacts
- Immunotherapy and Immune Responses
- Multiple Sclerosis Research Studies
- HIV Research and Treatment
- Cytomegalovirus and herpesvirus research
- Immunodeficiency and Autoimmune Disorders
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Polyomavirus and related diseases
- vaccines and immunoinformatics approaches
- Animal Virus Infections Studies
- Diabetes and associated disorders
- Full-Duplex Wireless Communications
- Hematopoietic Stem Cell Transplantation
- Consumer Attitudes and Food Labeling
- Cell Adhesion Molecules Research
- SARS-CoV-2 detection and testing
- Viral Infections and Immunology Research
- Systemic Sclerosis and Related Diseases
- Antioxidant Activity and Oxidative Stress
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
2015-2025
University of Brescia
1991-2023
University of Padua
2021
National Institutes of Health
2021
Istituto Superiore di Sanità
2010
LivaNova (Italy)
1999
Consorzio Interuniversitario per le Biotecnologie
1990-1997
National Research Council
1992-1993
Istituto di Tipizzazione Tissutale e Problemi della Dialisi
1990
T cells are involved in the early identification and clearance of viral infections also support development antibodies by B cells. This central role for makes them a desirable target assessing immune response to SARS-CoV-2 infection. Here, we combined two high-throughput profiling methods create quantitative picture T-cell SARS-CoV-2. First, at individual level, deeply characterized 3 acutely infected 58 recovered COVID-19 subjects experimentally mapping their CD8 through antigen stimulation...
Immune system aging is becoming a field of increasing public health interest because prolonged life expectancy, which not paralleled by an increase in expectancy. As age progresses, innate and adaptive immune systems undergo changes, are defined, respectively, as inflammaging senescence. A wealth available data demonstrates that these two conditions closely linked, leading to greater vulnerability elderly subjects viral, bacterial, opportunistic infections well lower post-vaccination...
Abstract Synovial fluids of patients with rheumatoid arthritis contain activated T lymphocytes that may play an important role in the pathogenesis disease. Previous studies have suggested cell receptor (TcR) repertoire these cells is restricted, reflecting vivo selection a limited number specificities at site inflammation. To characterize better populations we used polymerase chain reaction technology to estimate proportion TcR α and β RNA containing any particular V elements from...
Life-threatening “breakthrough” cases of critical COVID-19 are attributed to poor or waning antibody (Ab) response SARS-CoV-2 vaccines in individuals already at risk. Preexisting auto-Abs neutralizing type I IFNs underlie least 15% pneumonia unvaccinated individuals; their contribution hypoxemic breakthrough vaccinated people is unknown. We studied a cohort 48 (aged 20 86 years) who received two doses messenger RNA (mRNA) vaccine and developed infection with 2 weeks 4 months later. Ab levels...
SEN virus (SEN-V) is a recently identified single-stranded, circular DNA virus. Two SEN-V variants (SENV-D and SENV-H) were assayed by polymerase chain reaction (PCR) to investigate their role in the causation of transfusion-associated non-A E hepatitis. The incidence infection after transfusion was 30% (86 286) compared with 3% (3 97) among nontransfused controls (P < .001). Transfusion risk increased number units transfused .0001) donor-recipient linkage for shown sequence homology....
Abstract The ability of patients with hematologic malignancies (HM) to develop an effective humoral immune response after COVID-19 is unknown. A prospective study was performed monitor the SARS-CoV-2 follicular lymphoma (FL), diffuse large B-cell (DLBCL), chronic lymphoproliferative disorders (CLD), multiple myeloma (MM), or myelodysplastic/myeloproliferative syndromes (MDS/MPN). Antibody (Ab) levels nucleocapsid (N) and spike (S) protein were measured at +1, +3, +6 months nasal swabs became...
Dysregulation in neutrophil extracellular trap (NET) formation and degradation may play a role the pathogenesis severity of COVID-19; however, its pediatric manifestations this disease, including multisystem inflammatory syndrome children (MIS-C) chilblain-like lesions (CLLs), otherwise known as "COVID toes," remains unclear. Studying multinational cohorts, we found that, CLLs, NETs were significantly increased serum skin. There was geographic variability prevalence MIS-C, association with...
The lack of adenosine deaminase (ADA) leads to the accumulation toxic metabolites, resulting in SCID. If disease is left untreated, it likely have a fatal outcome early infancy. Because hematopoietic stem cell transplantation (HSCT) and enzyme replacement therapy with pegylated bovine ADA (PEG-ADA) are both provided our hospital, we undertook retrospective longitudinal comparative study extent lymphocyte recovery two groups treated ADA-SCID children. Together classical immunological...
Combination of chemotherapy and immunotherapy to increase the effectiveness an antitumor immune response is currently regarded as attractive strategy. In a pilot clinical trial, we have recently documented melanoma antigen A (Melan-A)-specific, tumor-reactive, long-lasting effector-memory CD8(+) T cells after administration dacarbazine (DTIC) 1 day before peptide vaccination in patients. Global transcriptional analysis revealed DTIC-induced activation genes involved leukocyte activation. To...
A 31-year-old woman affected by multiple sclerosis (MS) experienced generalized tonic–clonic seizures 2 months after the second alemtuzumab cycle. Positive JC virus (JCV)-DNA in cerebrospinal fluid (CSF) and lesion iconography at magnetic resonance imaging (MRI) were suggestive of progressive multifocal leukoencephalopathy (PML). After 1 month, during full-blown immune reconstitution inflammatory syndrome, JCV-DNA became negative symptoms gradually improved. New T- B-cell output diversity...
Background: Fingolimod inhibits lymphocyte egress from lymphoid tissues, thus altering the composition of peripheral pool multiple sclerosis patients. Objective: The objective this paper is to evaluate whether fingolimod determines a decrease newly produced T- and B-lymphocytes in blood reduction T-cell receptor repertoire diversity that may affect immune surveillance. Methods: Blood samples were obtained patients before therapy initiation then after six 12 months. Newly T B lymphocytes...
Abstract Background Assessment of T-cell diversity, besides giving insights about the molecular basis tumor antigen recognition, has clinical implications since it provides criteria for evaluating antigen-specific T cells clinically relevant spontaneous and vaccine-induced anti-tumor activity. Melan-A is one melanoma antigens most frequently recognized by peripheral tumor-infiltrating lymphocytes in HLA-A2+ patients. Many trials involving vaccination have been conducted using modified...
Abstract Background Coronavirus disease 2019 (COVID-19) severity seems to be influenced by genetic background, sex, age, and presence of specific comorbidities. So far, little attention has been paid sex-specific variations demographic, clinical, laboratory features COVID-19 patients referred the same hospital in two consecutive pandemic waves. Methods Demographic, data were collected 1000 (367 females 633 males), 500 hospitalized first wave second one, at ASST Spedali Civili Brescia from...
Progressive multifocal leukoencephalopathy (PML) usually occurs in patients with severe immunosuppression, hematological malignancies, chronic inflammatory conditions or receiving organ transplant. Recently, PML has also been observed treated monoclonal antibodies. By taking advantage of the availability samples from a multiple sclerosis (MS) patient natalizumab, antibody anti-α4 integrin, who developed and was monitored starting before therapy initiation, we investigated fate T B...
Abstract Background The immune system reconstitution in HIV-1- infected patients undergoing combined antiretroviral therapy is routinely evaluated by T-cell phenotyping, even though the infection also impairs B-cell mediated immunity. To find new laboratory markers of effectiveness, both B- and T- recovery were means a follow-up study long-term treated patients, with special focus on measure T-lymphocyte production. Methods A longitudinal analysis was performed samples obtained from...
To document the prevalence and routes of transmission SEN virus (SEN-V) in community-based individuals patients referred to a liver disease unit, stored serum samples obtained from 160 Canadian Inuit 140 with were tested for SEN-V DNA by polymerase chain reaction. In population, was present 57 (36%) persons. SEN-V-positive tended be younger more often male. Liver enzyme levels serologic markers hepatitis A B viruses similar SEN-V-negative individuals. 30 (21%) disease. Age, sex, risk factors...