A5065787721- Macrophage Migration Inhibitory Factor
- Nuclear Receptors and Signaling
- Endometriosis Research and Treatment
- Pregnancy and preeclampsia studies
- Vagus Nerve Stimulation Research
- Birth, Development, and Health
- Nicotinic Acetylcholine Receptors Study
- Cardiovascular Issues in Pregnancy
- Reproductive System and Pregnancy
- Traumatic Brain Injury and Neurovascular Disturbances
- Respiratory Support and Mechanisms
- Preterm Birth and Chorioamnionitis
- Apelin-related biomedical research
- Pregnancy-related medical research
- Diabetes and associated disorders
- Magnesium in Health and Disease
- Cell Adhesion Molecules Research
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Heart Rate Variability and Autonomic Control
- Neuroendocrine regulation and behavior
- Trypanosoma species research and implications
- Protease and Inhibitor Mechanisms
- Vascular Anomalies and Treatments
- Chemotherapy-induced organ toxicity mitigation
Feinstein Institute for Medical Research
2016-2025
Northwell Health
2016-2025
Donald & Barbara Zucker School of Medicine at Hofstra/Northwell
2017-2024
Universität Hamburg
2023-2024
University Medical Center Hamburg-Eppendorf
2023-2024
Icahn School of Medicine at Mount Sinai
2014-2022
North Shore University Hospital
2004-2021
Northeast Georgia Medical Center
2021
Coney Island Hospital
2021
General Hospital of Serres
2021
Macrophage migration inhibitory factor (MIF) accounts for one of the first cytokine activities to have been described, and it has emerged recently be an important regulator innate adaptive immunity. MIF is upstream activator monocytes/macrophages, centrally involved in pathogenesis septic shock, arthritis, other inflammatory conditions. The protein encoded by a unique but highly conserved gene, X-ray crystallography studies shown define new fold structural superfamily. Although recent work...
Abstract Fibrocytes are a distinct population of blood-borne cells that display unique cell surface phenotype (collagen I+/CD11b+/CD13+/CD34+/CD45RO+/MHC class II+/CD86+) and exhibit potent immunostimulatory activities. Circulating fibrocytes rapidly enter sites tissue injury, suggesting an important role for these in wound repair. However, the regulatory processes govern differentiation mechanisms underlie migration to currently not known. We report herein ex vivo cultured can differentiate...
Transforming growth factor-β (TGF-β) is a potent regulator of cellular differentiation, proliferation, migration, and protein expression. These properties have been exploited to create variety bioassays for detecting the mature factor. In this paper, we describe highly sensitive specific, nonradioactive quantitative bioassay TGF-β based on its ability induce plasminogen activator inhibitor-1 (PAI-1) Mink lung epithelial cells (MLEC) were stably transfected with an expression construct...
The protein known as macrophage migration inhibitory factor (MIF) was one of the first cytokines to be discovered and described 30 years ago a T-cell-derived that inhibited random macrophages in vitro. A much broader role for MIF has emerged recently result studies have demonstrated it released from anterior pituitary gland vivo. also is been identified secreted monocytes/macrophages upon glucocorticoid stimulation. Once released, acts "override" or counter-regulate suppressive effects...
Circulating autoantibodies (auto-Abs) neutralizing high concentrations (10 ng/mL, in plasma diluted 1 to 10) of IFN-α and/or -ω are found about 10% patients with critical COVID-19 pneumonia, but not subjects asymptomatic infections. We detect auto-Abs 100-fold lower, more physiological, (100 pg/mL, 1/10 dilutions plasma) 13.6% 3,595 COVID-19, including 21% 374 > 80 years, and 6.5% 522 severe COVID-19. These antibodies also detected 18% the 1,124 deceased (aged 20 days-99 years; mean: 70...
Endothelial cell activation plays a critical role in regulating leukocyte recruitment during inflammation and infection. Based on recent studies showing that acetylcholine other cholinergic mediators suppress the production of proinflammatory cytokines via α7 nicotinic receptor (α7 nAChR) expressed by macrophages our observations human microvascular endothelial cells express nAChR, we examined effect stimulation vitro vivo. Using Shwartzman reaction, observed nicotine (2 mg/kg) novel agent...
Macrophage migration inhibitory factor (MIF) is an important pro-inflammatory mediator with the unique ability to counter-regulate effects of glucocorticoids on immune cell activation. MIF released from cells in response glucocorticoids, certain stimuli, and mitogens acts regulate glucocorticoid action ensuing inflammatory response. To gain insight into molecular mechanism action, we have examined role proliferation intracellular signaling events well characterized, NIH/3T3 fibroblast line....
Transforming growth factor-β (TGF-β) is secreted by many cell types as part of a large latent complex composed three subunits: TGF-β, the TGF-β propeptide, and binding protein (LTBP). To interact with its surface receptors, must be released from disrupting noncovalent interactions between mature propeptide. Previously, we identified LTBP-1 transglutaminase, cross-linking enzyme, reactants involved in formation TGF-β. In this study, demonstrate that are substrates for transglutaminase....
Significance There is growing evidence that preexisting autoantibodies neutralizing type I interferons (IFNs) are strong determinants of life-threatening COVID-19 pneumonia. It important to estimate their quantitative impact on mortality upon SARS-CoV-2 infection, by age and sex, as both the prevalence these risk death increase with higher in men. Using an unvaccinated sample 1,261 deceased patients 34,159 individuals from general population, we found against IFNs strongly increased...
We recently described a novel population of blood-borne cells, termed fibrocytes, that display distinct cell surface phenotype (collagen+/CD13+/CD34+/CD45+), rapidly enter sites tissue injury, and contribute to scar formation. To further characterize the role these cells in vivo, we examined expression type I collagen cytokine mRNAs by isolated from wound chambers implanted into mice. Five days after chamber implantation, CD34+ fibrocytes but not CD14+ monocytes or CD90+ T expressed mRNA for...
OBJECTIVE—Data from experimental studies have suggested that the increased formation of advanced glycation end products (AGEs) is one causes endothelial dysfunction in diabetes. This study was performed to investigate whether changes endothelium-dependent vasodilation, a marker function, were related serum AGEs concentrations patients with type 2 RESEARCH DESIGN AND METHODS—For this study, 170 diabetes and 83 healthy nondiabetic control subjects similar age recruited. Serum assayed by...
Objective Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine whose involvement in tumor necrosis α (TNFα) synthesis and T cell activation suggests role the pathogenesis of rheumatoid arthritis (RA). Antagonism MIF associated with marked inhibition animal models RA. Uniquely, inducible by low concentrations glucocorticoids. We sought to investigate expression RA synovial tissue. Methods was demonstrated human synovium immunohistochemistry, flow cytometry, enzyme-linked...
MIF is a proinflammatory cytokine that has been implicated in the pathogenesis of sepsis, arthritis, and other inflammatory diseases. Antibodies against are effective experimental models inflammation, there interest strategies to inhibit its deleterious activities. Here we identify mechanism inhibiting pro-inflammatory activities by targeting tautomerase activity. We designed small molecules this activity; lead molecule, “ISO-1 ((S,R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid...