A5083364929- Immune Response and Inflammation
- Mycobacterium research and diagnosis
- Milk Quality and Mastitis in Dairy Cows
- Urinary Bladder and Prostate Research
- Probiotics and Fermented Foods
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Reproductive tract infections research
- Antimicrobial Peptides and Activities
- Immunotherapy and Immune Responses
- Genital Health and Disease
- Sexual function and dysfunction studies
- Hormonal and reproductive studies
- Veterinary Equine Medical Research
- Biopolymer Synthesis and Applications
- Reproductive Physiology in Livestock
- Bacterial biofilms and quorum sensing
- T-cell and B-cell Immunology
- Urinary Tract Infections Management
- Nanocomposite Films for Food Packaging
- Reproductive System and Pregnancy
- Macrophage Migration Inhibitory Factor
- Enterobacteriaceae and Cronobacter Research
- Identification and Quantification in Food
- Arsenic contamination and mitigation
Consejo Nacional de Investigaciones Científicas y Técnicas
2011-2025
National University of Villa María
2017-2025
Instituto Multidisciplinario de Biología Vegetal
2023-2024
Universidad Nacional de Córdoba
2010-2020
Research Centre in Biological Chemistry of Córdoba
2019-2020
Centro Científico Tecnológico - Tucumán
2020
Villa Maria Hospital
2020
Ghent University
2011
To assess the presence of self-reactive immune responses to seminal and prostate antigens (PAg), biomarkers inflammation male genital tract, semen quality parameters in patients with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS).Peripheral blood samples were collected from CP/CPPS age-matched healthy control volunteers. We analysed lymphoproliferative peripheral mononuclear cells (PBMC) different plasma (SP)-derived purified PAg, serum autoantibodies specific leucocyte...
Experimental autoimmune prostatitis (EAP) is considered a valid model for the human disease chronic prostatitis/chronic pelvic pain syndrome. In this report, we analyzed phenotypic characteristics of T cells that gain access to prostate and induce leukocyte recruitment in mice with different susceptibility EAP. After EAP induction, NOD developed specific cellular response characterized by mixed Th1/Th17 pattern mainly expressing CXCR3 infiltrated damaged prostate. contrast, BALB/c mice, as...
Experimental autoimmune prostatitis (EAP) is an inflammatory disease of the prostate characterized by peripheral prostate-specific responses associated with inflammation. EAP induced in rodents upon immunization antigens (PAg) plus adjuvants and shares important clinical immunological features human chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS).EAP was young NOD, C57BL/6, BALB/c male mice PAg complete Freund́s adjuvant. Tactile allodynia assessed using Von Frey fibers as a...
Toll-like receptor (TLR) ligands may be a valuable tool to promote antitumor responses by reinforcing immunity. In addition their expression in immune cells, functional TLRs are also expressed many cancer but significance has been controversial. this study, we examined the action of TLR on tumor pathophysiology as result direct cell effects. B16 murine melanoma cells were stimulated vitro with TLR4 ligand (LPS-B16) prior inoculation into TLR4-deficient mice (Tlr4 (lps-del)). Under such...
Pain and inflammation in the absence of infection are hallmarks chronic prostatitis pelvic pain syndrome (CP/CPPS) patients. The etiology CP/CPPS is unclear, autoimmunity has been proposed as a cause. Experimental autoimmune (EAP) models have long used for studying CP/CPPS. Herein, we studied prostate induction development EAP using IL-12p40-KO, IL-4-KO, IL-17-KO, wild-type (C57BL/6) mice. Prostate antigen (PAg) immunization C57BL/6 mice induced specific Th1 Th17 immune responses severe cell...
Abstract Bovine mastitis affects the health of dairy cows and profitability herds worldwide. Coagulase-negative staphylococci (CNS) are most frequently isolated pathogens in bovine intramammary infection. Based on wide range antimicrobial, mucoadhesive immunostimulant properties demonstrated by chitosan, we have evaluated therapy efficiency chitosan incorporation to cloxacillin antibiotic as well its effect against different bacterial lifestyles seven CNS isolates from chronic infections....
We investigated Chlamydia trachomatis infection and its pathogenic consequences in the male rodent genital tract.Male rats were inoculated meatal urethra with muridarum. sought bacterial DNA at early late times after inoculation different parts of tract. Histological alterations immune response against prostate antigens analyzed.Male showed ascending wide dissemination bacteria tract an time point inoculation. At later stages persisted only some gland. C. muridarum was also detected semen a...
The greatest concern in dairy farming nowadays is bovine mastitis (BM), which results mainly from bacterial colonization of the mammary gland. Antibiotics are most widely used strategy for its prevention and treatment, but overuse has led to growing antimicrobial resistance. Pathogens have also developed other mechanisms persist udder, such as biofilm formation internalization into epithelial cells. New therapies therefore needed reduce or replace antibiotic therapies. In a previous study,...
The role of innate cells and their receptors within the male genital tract remains poorly understood. Much less is known about relative contribution different such as epithelial/stromal resident leucocytes. In this study, we examined immune responses to Chlamydia trachomatis by prostate Murine primary cultures were performed leucocyte sorted based on surface protein expression CD45 magnetism-activated cell sorting or fluorescence-activated sorting. Prostate derived CD45- CD45+ infected with...
Macrophages contribute in the initiation and progression of insulitis during type 1 diabetes (T1D). However, mechanisms governing their recruitment into islets as well manner retention activation are incompletely understood. Here, we investigated a role for macrophage migration inhibitory factor (MIF) its transmembrane receptor, CD74, T1D. Our data indicated elevated MIF concentrations especially long-standing T1D patients mice. Additionally, NOD mice featured increased gene expression CD74+...
Abstract Susceptibility to autoimmune diseases results from the encounter of a complex and long evolved genetic context with no less changing environment. Major actors in maintaining health are regulatory T cells (Treg) that primarily dampen large subset autoreactive lymphocytes escaping thymic negative selection. Here, we directly asked whether Treg participate defining susceptibility resistance Experimental Autoimmune Prostatitis (EAP). We analyzed three common laboratory strains mice...
Chlamydia trachomatis is an intracellular pathogen that infects mucosal epithelial cells, causing persistent infections. Although chronic inflammation a hallmark of chlamydial disease, the proinflammatory mechanisms involved are poorly understood. Little known about how innate immunity in male genital tract (MGT) responds to C. trachomatis. Toll-like receptors (TLRs) family recognize different pathogen-associated molecular patterns (PAMPs) present bacteria, viruses, yeasts and parasites. The...
A significant proportion of individuals develop chronic, persistent and recurrent genital tract infections with Chlamydia trachomatis, which has been attributed to the numerous strategies that bacterium uses subvert host immune responses. Animal chlamydia models have demonstrated protective response is mediated by CD4+ Th1 cytokine Herein, we demonstrate early after infecting male tract, C. muridarum triggers production IL-10 splenic lymph node cells. In addition, IL-6 TNFα secretion. Data...
Bovine mastitis is the most frequent and costly disease that affects dairy cattle. Non-aureus staphylococci (NAS) are currently one of main pathogens associated with difficult-to-treat intramammary infections. Biofilm an important virulence factor can protect bacteria against antimicrobial treatment prevent their recognition by host's immune system. Previously, we found chronic isolates which were refractory to antibiotic therapy developed strong biofilm biomass. Now, evaluated influence...