A5057488889- Microtubule and mitosis dynamics
- Biotechnology and Related Fields
- Genetics, Bioinformatics, and Biomedical Research
- BRCA gene mutations in cancer
- Milk Quality and Mastitis in Dairy Cows
- Cancer Research and Treatments
- Mitochondrial Function and Pathology
- Probiotics and Fermented Foods
- Endoplasmic Reticulum Stress and Disease
- Cancer Genomics and Diagnostics
- Cellular transport and secretion
- Lipid metabolism and biosynthesis
- RNA modifications and cancer
- Protein Kinase Regulation and GTPase Signaling
- Digestive system and related health
- Metabolism and Genetic Disorders
- ATP Synthase and ATPases Research
- Nuclear Structure and Function
- RNA Research and Splicing
- Biopolymer Synthesis and Applications
- Cancer-related Molecular Pathways
Consejo Nacional de Investigaciones Científicas y Técnicas
2014-2024
Instituto Multidisciplinario de Biología Vegetal
2023-2024
National University of Villa María
2024
Universidad Nacional de Córdoba
2014-2020
Research Centre in Biological Chemistry of Córdoba
2014-2020
BRCA1 and BRCA2 deficiencies are widespread drivers of human cancers that await the development targeted therapies. We aimed to identify novel synthetic lethal relationships with therapeutic potential using BRCA-deficient isogenic backgrounds.We developed a phenotypic screening technology simultaneously search for (SL) interactions in BRCA1- BRCA2-deficient contexts. For validation, we chimeric spheroids dual-tumor xenograft model allowed confirmation SL induction concomitant evaluation...
Differentiation of neuronal cells is crucial for the development and function nervous system. This process involves high rates membrane expansion, during which synthesis lipids must be tightly regulated. In this work, using a variety molecular biochemical assays approaches, including immunofluorescence microscopy FRET analyses, we demonstrate that proto-oncogene c-Fos (c-Fos) activates cytoplasmic lipid in central system thereby supports differentiation. Specifically, hippocampal primary...
c-Fos is a well-recognized member of the AP-1 (activator protein-1) family transcription factors. In addition to this canonical activity, we previously showed that cytoplasmic activates phospholipid synthesis through mechanism independent its genomic activity. associates with particular enzymes lipid pathway at endoplasmic reticulum and increases Vmax reactions without modifying Km values. This activation associated events differentiation proliferation require high rates membrane biogenesis....
Glioblastoma multiforme is the most aggressive type of tumor CNS with an overall survival rate approximately one year. Since this has not changed significantly over last 20 years, development new therapeutic strategies for treatment these tumors peremptory. The over-expression proto-oncogene c-Fos been observed in several including glioblastoma and usually associated a poor prognosis. Besides its genomic activity as AP-1 transcription factor, protein can also activate phospholipid synthesis...
This is an assay designed to examine the radioactive phosphorous incorporation when molecule being synthesized, which means that only de novo synthesized phospholipids can be detected. Thus, with this technique it possible detect in vitro phospholipid synthesis under different required experimental conditions respect controls (Guido and Caputto, 1990; Ferrero et al., 2014). There are types of lipids. Among them we find phospholipids, contain glycerol esterified two fatty acyl chains a...
<p>Extended methods including bionformatic analysis details and scripts used</p>
<p>Legends included in the figure file</p>
<p>Legends included in the figure file</p>
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<p>Full list of hits the BRCA1-deficient population in screening performed with PKIS2 library</p>
<p>Legends included in the figure file</p>
<p>Legends included in the figure file</p>
<p>Extended methods including bionformatic analysis details and scripts used</p>
<p>Full list of hits the BRCA1-deficient population in screening performed with PKIS2 library</p>
<p>Legends included in the figure file</p>
<p>Legends included in the figure file</p>
<p>Legends included in the figure file</p>
<p>Legends included in the figure file</p>
<p>Legends included in the figure file</p>
<p>Legends included in the figure file</p>
<div>AbstractPurpose:<p>BRCA1 and BRCA2 deficiencies are widespread drivers of human cancers that await the development targeted therapies. We aimed to identify novel synthetic lethal relationships with therapeutic potential using BRCA-deficient isogenic backgrounds.</p>Experimental Design:<p>We developed a phenotypic screening technology simultaneously search for (SL) interactions in BRCA1- BRCA2-deficient contexts. For validation, we chimeric spheroids dual-tumor...
<div>AbstractPurpose:<p>BRCA1 and BRCA2 deficiencies are widespread drivers of human cancers that await the development targeted therapies. We aimed to identify novel synthetic lethal relationships with therapeutic potential using BRCA-deficient isogenic backgrounds.</p>Experimental Design:<p>We developed a phenotypic screening technology simultaneously search for (SL) interactions in BRCA1- BRCA2-deficient contexts. For validation, we chimeric spheroids dual-tumor...