A5087142337- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Macrophage Migration Inhibitory Factor
- Viral Infectious Diseases and Gene Expression in Insects
- DNA and Nucleic Acid Chemistry
- Neuroscience and Neural Engineering
- Graphene and Nanomaterials Applications
- Nanowire Synthesis and Applications
Ghent University
2020-2022
Cancer Research Institute Ghent
2020
KU Leuven
2017
Small nucleic acid (NA) therapeutics, such as small interfering RNA (siRNA), are generally formulated in nanoparticles (NPs) to overcome the multiple extra- and intracellular barriers upon vivo administration. Interaction with target cells typically triggers endocytosis sequesters NPs endosomes, thus hampering pharmacological activity of encapsulated siRNAs that occurs cytosol. Unfortunately, for most state-of-the-art NPs, endosomal escape is largely inefficient. As a result, bulk...
Abstract Efficient and safe cell engineering by transfection of nucleic acids remains one the long-standing hurdles for fundamental biomedical research many new therapeutic applications, such as CAR T cell-based therapies. mRNA has recently gained increasing attention a more versatile alternative tool over viral- or DNA transposon-based approaches generation adoptive cells. However, limitations associated with existing nonviral delivery hamper progress on genetic these hard-to-transfect...
The CRISPR-Cas9 technology represents a powerful tool for genome engineering in eukaryotic cells, advancing both fundamental research and therapeutic strategies. Despite the enormous potential of technology, efficient direct intracellular delivery Cas9 ribonucleoprotein (RNP) complexes target cells poses significant hurdle, especially refractive primary cells. In present work, vapor nanobubble (VNB) photoporation was explored RNP transfection variety cell types. Proof concept first...
Intracellular delivery of membrane-impermeable cargo offers unique opportunities for biological research and the development cell-based therapies. Despite breadth available intracellular tools, existing protocols are often suboptimal alternative approaches that merge efficiency with both biocompatibility, as well applicability, remain highly sought after. Here, a comprehensive platform is presented exploits property cationic hydrogel nanoparticles to transiently disrupt plasma membrane...
Macrophages contribute in the initiation and progression of insulitis during type 1 diabetes (T1D). However, mechanisms governing their recruitment into islets as well manner retention activation are incompletely understood. Here, we investigated a role for macrophage migration inhibitory factor (MIF) its transmembrane receptor, CD74, T1D. Our data indicated elevated MIF concentrations especially long-standing T1D patients mice. Additionally, NOD mice featured increased gene expression CD74+...