A5084809303- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Immunotherapy and Immune Responses
- 3D Printing in Biomedical Research
- Extracellular vesicles in disease
- Virus-based gene therapy research
- Nanowire Synthesis and Applications
- Graphene and Nanomaterials Applications
- Nanoparticle-Based Drug Delivery
- CRISPR and Genetic Engineering
- Nanofabrication and Lithography Techniques
- Electrophoretic Deposition in Materials Science
- Atomic and Subatomic Physics Research
- Quantum Dots Synthesis And Properties
- Microfluidic and Bio-sensing Technologies
- Electrospun Nanofibers in Biomedical Applications
- Nanocluster Synthesis and Applications
- Carbon and Quantum Dots Applications
- Luminescence Properties of Advanced Materials
- Intraocular Surgery and Lenses
- Laser-Ablation Synthesis of Nanoparticles
- Moyamoya disease diagnosis and treatment
- Vascular Malformations Diagnosis and Treatment
Ghent University
2019-2024
Charles University
2023
Ghent University Hospital
2021
Abstract Safe and efficient production of chimeric antigen receptor (CAR)‐T cells is crucial importance for cell‐based cancer immunotherapy. Physical transfection methods have quickly gained in the context transfecting T‐cells, since they are readily compatible with different cell types a broad variety cargo molecules. In particular, nanoparticle‐sensitized photoporation has been introduced recent years as gentle yet method to transiently permeabilize cells, allowing subsequent entry...
Abstract Photodynamic and photothermal cell killing at the surface of tissues finds applications in medicine. However, a lack control over heat dissipation following treatment with light might damage surrounding tissues. A new strategy to kill cells is reported. Polymeric films are designed which iron oxide nanoparticles embedded as photosensitizers. Irradiation pulsed laser generates water vapor bubbles films. It found that “bubble‐films” can close proximity due mechanical forces arise when...
Photoporation is an up-and-coming technology for the gentle and efficient transfection of cells. Inherent to application photoporation optimization several process parameters, such as laser fluence sensitizing particle concentration, which typically done one factor at a time (OFAT). However, this approach tedious runs risk missing global optimum. Therefore, in study, we explored whether response surface methodology (RSM) would allow more procedure. As case FITC-dextran molecules 500 kDa were...
Nanoparticle mediated laser-induced photoporation is a physical cell membrane disruption approach to directly deliver extrinsic molecules into living cells, which particularly promising in applications for both adherent and suspension cells. In this work, we explored surface modifications of graphene quantum dots (GQD) reduced oxide (rGO) with polyethylene glycol (PEG) polyethyleneimine (PEI) enhance colloidal stability while retaining functionality. After FITC-dextran 10 kDa (FD10), the...
The CRISPR-Cas9 technology represents a powerful tool for genome engineering in eukaryotic cells, advancing both fundamental research and therapeutic strategies. Despite the enormous potential of technology, efficient direct intracellular delivery Cas9 ribonucleoprotein (RNP) complexes target cells poses significant hurdle, especially refractive primary cells. In present work, vapor nanobubble (VNB) photoporation was explored RNP transfection variety cell types. Proof concept first...
Laser-induced nanoparticle-sensitized photoporation of hard-to-transfect primary macrophages for the <italic>ex vivo</italic> delivery inflammasome inducing toxins.
PEGylation, which has traditionally been the method of choice to enhance colloidal stability nanostructures designed for biological applications and prevent nonspecific protein adsorption, is now being challenged by short zwitterionic ligands. Inspired nature cell membranes, these ligands have potential push forward field nanoparticles nanomedicine. In this work, we report a thorough analysis surface chemistry silica-coated luminescent CdSe/CdS quantum dots functionalized with either...
Abstract Dendritic cell (DC)‐based vaccines for immunotherapy have already achieved promising results in the last decade. To further improve current treatment protocols and enhance therapeutic outcome, noninvasive vivo tracking of DCs remains crucial importance. Persistent luminescent nanoparticles (PLNPs) are inorganic materials which show an afterglow hours after optical excitation has ceased. If is near‐infrared, emission injected particles can be tracked vivo. However, stability toxicity...
Intracellular delivery of membrane-impermeable cargo offers unique opportunities for biological research and the development cell-based therapies. Despite breadth available intracellular tools, existing protocols are often suboptimal alternative approaches that merge efficiency with both biocompatibility, as well applicability, remain highly sought after. Here, a comprehensive platform is presented exploits property cationic hydrogel nanoparticles to transiently disrupt plasma membrane...
Nanoparticle-sensitized photoporation for intracellular delivery of external compounds usually relies on the use spherical gold nanoparticles as sensitizing nanoparticles. As they need stimulation with visible laser light, are less suited transfection cells in thick biological tissues. In this work, we have explored black phosphorus quantum dots (BPQDs) alternative a broad and uniform absorption spectrum from to near infra-red (NIR) range. We demonstrate that BPQD sensitized allows efficient...
Nanoparticle-sensitized photoporation for safe and efficient cytosolic delivery of gadolinium chelates, enabling high-contrast <italic>in vivo</italic> cell tracking cells by magnetic resonance imaging.
In article number 2008379, Ranhua Xiong, Chaobo Huang, Stefaan C. De Smedt, Félix Sauvage, and co-workers introduce a laser-responsive polymeric film for spatial selective cell killing. Iron oxide clusters embedded in the can absorb laser energy generate vapor bubbles. Single-cell killing be achieved at surface of eye by mechanical forces that arise upon collapse These bubble forming films have applications as adjuvants after cancer surgery are promising safe resection superficial tumors.