A5061866349- Medical Imaging Techniques and Applications
- Advanced MRI Techniques and Applications
- Advanced X-ray and CT Imaging
- Radiomics and Machine Learning in Medical Imaging
- Cardiac Imaging and Diagnostics
- Glioma Diagnosis and Treatment
- Radiopharmaceutical Chemistry and Applications
- Cancer, Hypoxia, and Metabolism
- Functional Brain Connectivity Studies
- Advanced Radiotherapy Techniques
- Cytokine Signaling Pathways and Interactions
- Neuroscience and Neuropharmacology Research
- Monoclonal and Polyclonal Antibodies Research
- Nanoplatforms for cancer theranostics
- Advanced Neuroimaging Techniques and Applications
- Neurological disorders and treatments
- Brain Metastases and Treatment
- Intraperitoneal and Appendiceal Malignancies
- Nanoparticle-Based Drug Delivery
- Radiation Dose and Imaging
- Cardiovascular Function and Risk Factors
- Drug Transport and Resistance Mechanisms
- Estrogen and related hormone effects
- Liver Disease Diagnosis and Treatment
- Neural dynamics and brain function
Ghent University
2016-2025
Infinity (United States)
2014-2025
Ghent University Hospital
2014-2024
Signal Processing (United States)
2014-2024
Cancer Research Institute Ghent
2017-2023
Institute of Technologists
2021
iMinds
2012-2019
Creative Research Enterprises (United States)
2019
Institut Gustave Roussy
2019
University of Turin
2019
Human epidermal growth factor receptor 2 (HER2) status is one of the major tumor characteristics in breast cancer to guide therapy. Anti-HER2 treatment has clear survival advantages HER2-positive carcinoma patients. Heterogeneity HER2 expression between primary and metastasis repeatedly been described, resulting need reassess during disease course. To avoid repeated biopsy with potential bias due heterogeneity, Nanobodies directed against have developed as probes for molecular imaging....
Camelidae possess an unusual class of antibodies devoid light chains. Nanobodies are intact antigen-binding fragments that stable, easily generated against different targets, and fully functional. Their rapid clearance from the blood circulation favors their use as imaging agents. We compared in vivo tumor uptake biodistribution 2 anti–epidermal growth factor receptor (anti-EGFR) Nanobodies, <sup>99m</sup>Tc-7C12 <sup>99m</sup>Tc-7D12. <b>Methods:</b> were labeled via hexahistidine tail with...
Event Abstract Back to Changes in functional brain networks during epileptogenesis a rodent model of temporal lobe epilepsy Emma Christiaen1*, Marie-Gabrielle Goossens2, Benedicte Descamps1, Paul Boon2, Robrecht Raedt2 and Christian Vanhove1 1 Ghent University - IMEC, Department Electronics Information Systems, Belgium 2 University, Neurology, Introduction Temporal (TLE) is the most common form adults. Research has shown that abnormal are involved development generation spread seizures....
Abstract Background Nanobodies are single‐domain antigen binding fragments derived from functional heavy‐chain antibodies elicited in Camelidae. They powerful probes for radioimmunoimaging, but their renal uptake is relatively high. In this study we have evaluated the role of megalin on anti‐EGFR 99m Tc‐7C12 nanobody and potency gelofusine and/or lysine to reduce Tc‐7C12. Methods First compared megalin‐deficient megalin‐wild‐type mice using pinhole SPECT/microCT ex vivo analysis. The effect...
Abstract Preoperative radiotherapy (RT) is a mainstay in the management of rectal cancer, tumor characterized by desmoplastic stroma containing cancer-associated fibroblasts (CAF). Although CAFs are abundantly present, effects RT to CAF and its impact on cancer cells unknown. We evaluated damage responses investigated changes colorectal cell growth, transcriptome, metabolome, kinome response paracrine signals emerging from irradiated CAF. induced DNA damage, p53 activation, cell-cycle...
Increasing evidence supports the critical roles played by adipose tissue in breast cancer progression. Yet, mediators and mechanisms are poorly understood. Here, we show that cancer-associated from freshly isolated tumors promotes F-actin remodeling, cellular scattering, invasiveness, spheroid reorganization of cultured cells. A combination techniques, including transcriptomics, proteomics, kinomics enabled us to identify paracrine secretion oncostatin M (OSM) tissue. Specifically, OSM,...
A generic site‐specific conjugation method that generates a homogeneous product is of utmost importance in tracer development for molecular imaging and therapy. We explored the protein‐ligation capacity enzyme Sortase to label camelid single‐domain antibody‐fragments, also known as nanobodies. The versatility approach was demonstrated by conjugating independently three different probes: chelating agents CHX‐A"‐DTPA NOTA single‐photon emission computed tomography (SPECT) with indium‐111...
Angiogenesis contributes to the development of nonalcoholic steatohepatitis (NASH) and promotes inflammation, fibrosis, progression hepatocellular carcinoma (HCC). Angiopoietin‐2 (Ang‐2) is a key regulator angiogenesis. We aimed investigate role Ang‐2 its potential as therapeutic target in NASH using human samples, vivo mouse models, vitro assays. Serum levels were determined 104 obese patients undergoing bariatric surgery concomitant liver biopsy. The effect Ang‐2/Tie2 receptor inhibiting...
Positron emission tomography-computed tomography (PET-CT) is a highly sensitive clinical molecular imaging modality to study atherosclerotic plaque biology. Therefore, we sought develop new PET tracer, targeting vascular cell adhesion molecule (VCAM)-1 and validate it in murine model as potential agent detect inflammation.The anti-VCAM-1 nanobody (Nb) (cAbVCAM-1-5) was radiolabelled with Fluorine-18 ((18)F), radiochemical purity of >98%. In vitro cell-binding studies showed specific binding...
This paper describes an iterative algorithm for high-dimensional linear inverse problems, which is regularized by a differentiable discrete approximation of the total variation (TV) penalty. The interlaced method based on optimization transfer with separable quadratic surrogate TV cost function optimized using block algebraic reconstruction technique (RSART). A proof convergence given and illustrated numerical experiments simulated parallel-beam computerized tomography (CT) data. proposed...
Although histology has proven to be a reliable method evaluate the ossoeintegration of dental implant, it is costly, time consuming, destructive, and limited one or few sections. Microcomputed tomography (µCT) fast delivers three-dimensional information, but this technique not been widely used validated for histomorphometric parameters yet. This study compared µCT histomorphometry by means evaluating their accuracy in determining bone response two different implant materials. In total, 32...
The intraperitoneal (IP) administration of chemotherapy is an alternative treatment for peritoneal carcinomatosis, allowing higher intratumor concentrations the cytotoxic agent compared to intravenous administration. Nevertheless, drug penetration depths are still limited a few millimeters. It thus necessary better understand limiting factors behind this poor in order improve IP delivery. By developing three-dimensional computational fluid dynamics (CFD) model tumor nodule, we investigated...
Immune checkpoints, such as programmed death-ligand 1 (PD-L1), limit T-cell function and tumor cells use this ligand to escape the anti-tumor immune response. Treatments with monoclonal antibodies blocking these checkpoints have shown long-lasting responses, but only in a subset of patients. This study aims develop Nanobody (Nb)-based probe order assess human PD-L1 (hPD-L1) expression using positron emission tomography imaging, compare influence two different radiolabeling strategies, since...
Gliomas, the most frequent type of primary tumor central nervous system in adults, results significant morbidity and mortality. Despite development novel, complex, multidisciplinary, targeted therapies, glioma therapy has not progressed much over last decades. Therefore, there is an urgent need to develop novel patient-adjusted immunotherapies that actively stimulate antitumor T cells, generate long-term memory, result clinical benefits. This work aimed investigate efficacy molecular...
Abstract Introduction Micro‐CT provides non‐invasive anatomic evaluation of small animals. Serial micro‐CT measurements are, however, hampered by the severity ionizing radiation doses cumulating over total period follow‐up. The dose levels may be sufficient to influence experimental outcomes such as animal survival or tumor growth. Aim This study was designed evaluate and optimize scanning protocol for longitudinal scans. Methods Materials Normal C57Bl/6 mice were euthanized. Radiation...
Total variation (TV) methods have been proposed to improve the image quality in count-reduced images, by reducing between neighboring pixels. Although very easy implement and fast compute, TV-based may lead a loss of texture information when applied images with complex textures, such as high-resolution abdominal CT images. Here, we investigate use another regularization approach context medical based on multiresolution transformations. One transformation is shearlet transform, which...
Abstract Background Functional disturbances of the endoplasmic reticulum (ER) lead to activation unfolded protein response (UPR), which is involved in consecutive steps carcinogenesis. In human hepatocellular carcinoma (HCC), UPR shown be activated; however, little known about kinetics and effects modulation HCC. Methods We sequentially monitored over time an orthotopic mouse model for HCC explored on cell viability proliferation vitro model. Results The expression ER-resident chaperones...
// Félix Gremonprez 1 , Benedicte Descamps 2 Andrei Izmer 3 Christian Vanhove Frank Vanhaecke Olivier De Wever 4 Wim Ceelen Department of Surgery, Ghent University Hospital, Ghent, Belgium Infinity (iMinds-IBiTech-MEDISIP), Electronics and Information Systems, University, Atomic Mass Spectrometry, Analytical Chemistry, Radiation Oncology Experimental Cancer Research, Correspondence to: Gremonprez, e-mail: Felix.gremonprez@ugent.be Keywords: peritoneal carcinomatosis, colorectal cancer,...