A5107790408- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- DNA Repair Mechanisms
- Cancer, Lipids, and Metabolism
- Cancer Cells and Metastasis
- Cytokine Signaling Pathways and Interactions
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Risks and Factors
- Cancer, Hypoxia, and Metabolism
- Cardiovascular Disease and Adiposity
- Metabolism, Diabetes, and Cancer
- Extracellular vesicles in disease
- Mesenchymal stem cell research
- Epigenetics and DNA Methylation
- Estrogen and related hormone effects
- Fibroblast Growth Factor Research
- Cancer-related Molecular Pathways
- DNA and Nucleic Acid Chemistry
- Lipid metabolism and disorders
- Prostate Cancer Treatment and Research
- Cancer therapeutics and mechanisms
- Immune cells in cancer
- PARP inhibition in cancer therapy
- Carcinogens and Genotoxicity Assessment
- Chronic Lymphocytic Leukemia Research
Université de Toulouse
2015-2024
Université Toulouse III - Paul Sabatier
2014-2024
Institut de Pharmacologie et de Biologie Structurale
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Laboratoire de Chimie de Coordination
2023
La Ligue Contre le Cancer
2020-2022
Clinique Claude-Bernard
2022
de Duve Institute
2017
Institut de Biologie Structurale
2016
Inserm
2011-2013
Abstract Early local tumor invasion in breast cancer results a likely encounter between cells and mature adipocytes, but the role of these fat progression remains unclear. We show that murine human cocultivated with adipocytes exhibit increased invasive capacities vitro vivo, using an original two-dimensional coculture system. Likewise, cultivated also altered phenotype terms delipidation decreased adipocyte markers associated occurrence activated state characterized by overexpression...
Abstract Cancer-associated fibroblasts (CAF) comprise the majority of stromal cells in breast cancers, yet their precise origins and relative functional contributions to malignant progression remain uncertain. Local invasion leads proximity cancer adipocytes, which respond by phenotypical changes generate fibroblast-like termed as adipocyte-derived (ADF) here. These exhibit enhanced secretion fibronectin collagen I, increased migratory/invasive abilities, expression CAF marker FSP-1 but not...
In breast cancer, a key feature of peritumoral adipocytes is their loss lipid content observed both in vitro and human tumors. The free fatty acids (FFAs), released by after lipolysis induced tumor secretions, are transferred stored cells as triglycerides droplets. cell lines, we demonstrate that FFAs can be over time from droplets through an adipose triglyceride lipase–dependent (ATGL-dependent) lipolytic pathway. vivo, ATGL expressed tumors where its expression correlates with...
Abstract Malignant progression results from a dynamic cross-talk between stromal and cancer cells. Recent evidence suggests that this is mediated to significant extent by exosomes, nanovesicles secreted most cell types which allow the transfer of proteins, lipids, nucleic acids Adipocytes are major component several tumor microenvironments, including invasive melanoma, where cells have migrated adipocyte-rich hypodermic layer skin. We show adipocytes secrete exosomes in abundance, then taken...
Obesity favours the occurrence of locally disseminated prostate cancer in periprostatic adipose tissue (PPAT) surrounding gland. Here we show that adipocytes from PPAT support directed migration cells and this event is strongly promoted by obesity. This process dependent on secretion chemokine CCL7 adipocytes, which diffuses to peripheral zone prostate, stimulating CCR3 expressing tumour cells. In obesity, higher facilitates extraprostatic extension. The observed increase associated with...
Summary Exosomes are important mediators in cell‐to‐cell communication and, recently, their role melanoma progression has been brought to light. Here, we characterized exosomes secreted by seven cell lines with varying degrees of aggressivity. Extensive proteomic analysis confirmed the presence characteristic exosomal markers as well melanoma‐specific antigens and oncogenic proteins. Importantly, protein composition differed among from different lines. aggressive cells contained specific...
Increasing evidence supports the critical roles played by adipose tissue in breast cancer progression. Yet, mediators and mechanisms are poorly understood. Here, we show that cancer-associated from freshly isolated tumors promotes F-actin remodeling, cellular scattering, invasiveness, spheroid reorganization of cultured cells. A combination techniques, including transcriptomics, proteomics, kinomics enabled us to identify paracrine secretion oncostatin M (OSM) tissue. Specifically, OSM,...
Clinical studies suggest that obesity, in addition to promoting breast cancer aggressiveness, is associated with a decrease chemotherapy efficacy, although the mechanisms involved remain elusive. As one of main treatments for aggressive or metastatic cancer, we investigated whether adipocytes can mediate resistance doxorubicin (DOX), drugs used treat and associated. We coculture system grow cells vitro differentiated as well primary mammary isolated from lean obese patients. Drug cellular...
Abstract Prostate gland is surrounded by periprostatic adipose tissue (PPAT), which increasingly believed to play a paracrine role in prostate cancer progression. Our previous work demonstrates that adipocytes promote homing of cells PPAT and this effect upregulated obesity. Here, we show once tumor have invaded (mimicked an vitro model coculture), they establish bidirectional crosstalk with adipocytes, promotes cell invasion. Indeed, induce adipocyte lipolysis the free fatty acids (FFA)...
Under caloric restriction, bone marrow adipocytes (BM-Ads) do not decrease in size compared to white adipocytes, suggesting they harbor unique metabolic properties. We compare human primary BM-Ads with paired subcutaneous (SC-Ads) using proteomic and lipidomic approaches. find that, although SC-Ads share similar morphological features, possess distinct lipid metabolism. Although BM-Ad shows enrichment proteins involved cholesterol metabolism, correlating increased free content, lipolysis...
AbstractThe induction of DNA double-strand breaks (DSBs) by genotoxic treatment leads to hightoxicity and genetic instability. Various approaches have been undertaken quantify thenumber follow the kinetic DSB repair. Recently, phosphorylation ofthe variant histone H2AX (named γH2AX), quantified specific immunodetectionapproaches, has provided a valuable highly sensitive method monitor DSBs formation.Although it is admitted that number γH2AX foci reflected DSBs, contradictoryreports leave...
Adipose tissue is an active endocrine organ that produces a variety of secretory factors involved in the initiation angiogenic processes. The bioactive peptide apelin endogenous ligand G protein-coupled receptor, APJ. Here we investigated potential role and its APJ, responses human endothelial cells development functional vascular network model adipose mice. Treatment umbilical vein with dose-dependently increased responses, including cell migration, proliferation, Matrigel(R) capillary...
Growing evidence indicates that adiposity is associated with breast cancer risk and negatively affects recurrence survival, a paracrine role of mammary adipose tissue being very likely in this process. In contrast to other depots, occurrence sub-inflammatory state defined by dying adipocytes surrounded macrophages forming crown-like structures overweight obese subjects, remains only partially described. general population patients (107 patients) mostly undergoing breast-conserving surgery,...
The DNA-dependent protein kinase (DNA-PK) is required for double-strand break repair in mammalian cells. DNA-PK contains the heterodimer Ku and a 460-kDa serine/threonine catalytic subunit (p460). binds vitro to DNA termini or other discontinuities helix able enter molecule by an ATP-independent process. It clear from experiments that stimulates recruitment of p460 activates activity toward DNA-binding substrates vicinity. Here, we have examined human nuclear cell extracts influence on...