A5043172423- Adipose Tissue and Metabolism
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Neurological Disorders and Treatments
- Natural Antidiabetic Agents Studies
- Mitochondrial Function and Pathology
- Lipid metabolism and biosynthesis
- Diet and metabolism studies
- Peroxisome Proliferator-Activated Receptors
- Adipokines, Inflammation, and Metabolic Diseases
- Diet, Metabolism, and Disease
- Cancer, Lipids, and Metabolism
- Metabolism and Genetic Disorders
- Cancer-related molecular mechanisms research
- Lipid metabolism and disorders
- Cannabis and Cannabinoid Research
- Metabolomics and Mass Spectrometry Studies
- Genetics, Aging, and Longevity in Model Organisms
- Biochemical effects in animals
- Cancer, Hypoxia, and Metabolism
- Fatty Acid Research and Health
- Autophagy in Disease and Therapy
- Diabetes Treatment and Management
- MicroRNA in disease regulation
- Apelin-related biomedical research
Université de Montréal
2014-2024
Centre Hospitalier de l’Université de Montréal
2005-2024
Montreal Clinical Research Institute
2017
Université Laval
2006-2009
University of Ottawa
2006-2009
McGill University
2006
Steno Diabetes Centers
2005
University Hospital of Lausanne
1999
University of Alberta
1999
Boston University
1988-1996
In breast cancer, a key feature of peritumoral adipocytes is their loss lipid content observed both in vitro and human tumors. The free fatty acids (FFAs), released by after lipolysis induced tumor secretions, are transferred stored cells as triglycerides droplets. cell lines, we demonstrate that FFAs can be over time from droplets through an adipose triglyceride lipase–dependent (ATGL-dependent) lipolytic pathway. vivo, ATGL expressed tumors where its expression correlates with...
1. The effect of insulin upon glucose transport and metabolism in soleus muscles genetically obese (fa/fa) heterozygote lean Zucker rats was investigated at 5-6 weeks 10-11 age. Weight-standardized strips were used rather than the intact muscle order to circumvent problems diffusion substrates. 2. In younger (5-6 weeks), plasma concentrations immunoreactive twice those controls, whereas their circulating triacylglycerol normal. Insulin effects 2-deoxyglucose uptake by these characterized...
Changes in the concentration of malonyl-CoA many tissues have been related to alterations activity acetyl-CoA carboxylase (ACC), rate-limiting enzyme its formation. In contrast, little is known about physiological role decarboxylase (MCD), an responsible for catabolism. this study, we examined effects voluntary exercise on MCD rat liver, skeletal muscle, and adipose tissue. addition, ofsn-glycerol-3-phosphate acyltransferase (GPAT), which like ACC can be regulated by AMP-activated protein...
KLF11 (TIEG2) is a pancreas-enriched transcription factor that has elicited significant attention because of its role as negative regulator exocrine cell growth in vitro and vivo . However, functional the endocrine pancreas remains to be established. Here, we report, for first time, our knowledge, characterization glucose-inducible insulin gene. A combination random oligonucleotide binding, EMSA, luciferase reporter, chromatin immunoprecipitation assays shows binds promoter regulates...
The regulation of Ca2+ transport by intracellular compartments was studied in digitonin-permeabilized human neutrophils, using a Ca2+-selective electrode. When incubated medium containing ATP and respiratory substrates, the cells lowered within 6 min ambient [Ca2+] to steady state around 0.2 microM. A vesicular ATP-dependent vanadate-sensitive non-mitochondrial pool maintained this low level. In absence ATP, higher 0.6 microM seen, exhibiting characteristics mitochondrial set point. Both...
The mechanism by which long-term exposure of the β-cell to elevated concentrations fatty acid alters glucose-induced insulin secretion has been examined. Exposure INS-1 β-cells 0.4 mmol/l oleate for 72 h increased basal and decreased release in response high glucose, but not agents acting at level KATP channel (tolbutamide) or beyond (elevated KCl). This also suppressed increase cellular ATP-to-ADP ratio. depolarization plasma membrane promoted glucose was after exposure, whereas KCl...
Alterations in the concentration of malonyl-CoA, an inhibitor carnitine palmitoyltransferase I, have been linked to regulation fatty acid oxidation skeletal muscle. During contraction decreases muscle malonyl-CoA related activation AMP-activated protein kinase (AMPK), which phosphorylates and inhibits acetyl-CoA carboxylase (ACC), rate-limiting enzyme formation. We report here that activity decarboxylase (MCD) is increased contracting Using either immunopurified or partially purified by...
Evidence from epidemiological studies and animal models suggests a link between high levels of dietary fat intake risk breast cancer. In addition, obesity, in which circulating lipids are elevated, is associated with increased various cancers. Relative to this point, we previously showed that oleate stimulates the proliferation cancer cells phosphatidylinositol 3-kinase plays role process. Nonetheless, questions remain regarding precise mechanism(s) by promotes cell growth. Pharmacological...
The effects of phalloidin and cytochalasin D, drugs which, respectively, stabilize destabilize actin microfilaments, have been tested on isolated rat hepatocytes. Both produced a modification cell shape, characterized by protrusions bulging from the cytoplasm. In phalloidin-treated hepatocytes, an accumulation microfilamentous network was detectable at base each protrusion electron microscopy, immunofluorescence, HMM decoration. This microfilaments absent in D-treated cells. release...
Abstract The effect of the muscarinic agonist carbamylcholine on cytoplasmic Ca2+ concentration ([Ca2+]i was examined at single cell level in clonal pancreatic beta-cells (HIT). Cells were loaded with indicator dye fura 2, and [Ca2+]i measured by microfluorimetry. Carbamylcholine induced changes that differed from to provoked some cells oscillatory fluctuations. During a transient, free rose peak within 1-3 s. frequency oscillations increased concentration. Oscillations occurred absence...
Acute increases in the concentration of malonyl-CoA play a pivotal role mediating decrease fatty acid oxidation that occurs many tissues during refeeding after fast. In this study, we assess whether such liver could be mediated by decarboxylase (MCD), as well acetyl-CoA carboxylase (ACC). addition, examine how changes activity ACC, MCD, and other enzymes govern glycerolipid synthesis relate temporally to alterations activities fuel-sensing enzyme AMP-activated protein kinase (AMPK). Rats...
Fatty acids are important metabolic substrates for the pancreatic β-cell, and long term exposure of islets to elevated concentrations fatty results in an alteration glucose-induced insulin secretion. Previous work suggested that exaggerated acid oxidation may be implicated this process by a mechanism requiring changes enzyme expression. We have therefore studied regulation carnitine palmitoyltransferase I (CPT I) gene expression β-cell line INS-1 since catalyzes limiting step various...