A5013391930- Pancreatic function and diabetes
- Exercise and Physiological Responses
- Muscle metabolism and nutrition
- Pluripotent Stem Cells Research
- Diabetes Management and Research
- Metabolism, Diabetes, and Cancer
- Diabetes and associated disorders
- Sports Performance and Training
- Vitamin C and Antioxidants Research
- Cardiovascular and exercise physiology
- Diet, Metabolism, and Disease
- Diet and metabolism studies
- Mesenchymal stem cell research
- Sports injuries and prevention
- Vitamin D Research Studies
- Hibiscus Plant Research Studies
- Tissue Engineering and Regenerative Medicine
- Adipose Tissue and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Thermoregulation and physiological responses
- Nutrition and Health in Aging
- Genetics and Neurodevelopmental Disorders
- Cardiovascular Health and Disease Prevention
- Metabolism and Genetic Disorders
- High Altitude and Hypoxia
Universitat de Miguel Hernández d'Elx
2016-2025
Instituto de Salud Carlos III
2016-2025
Instituto de investigación sanitaria y biomédica de Alicante
2018-2024
Fundación Española de la Nutrición
2024
Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition
2013-2024
Centro de Investigación Biomédica en Red
2014-2023
Hospital General Universitario de Alicante Doctor Balmis
2023
Universidad de Valladolid
2022
Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana
2017-2020
Université de Montréal
1994-2009
Embryonic stem (ES) cells display the ability to differentiate in vitro into a variety of cell lineages. Using cell-trapping system, we have obtained an insulin-secreting clone from undifferentiated ES cells. The construction used allows expression neomycin selection system under control regulatory regions human insulin gene. chimeric gene also contained hygromycin resistance (pGK-hygro) select transfected A resulting (IB/3x-99) containing 16.5 ng/microg protein total displays regulated...
In most clinical trials, human mesenchymal stem cells (hMSCs) are expanded in vitro before implantation. The genetic stability of is critical for their use. However, the relationship between stem-cell expansion and poorly understood. Here, we demonstrate that within normal period, hMSC cultures show a high percentage aneuploid progressively increases until senescence. Despite this accumulation, heterogeneous culture senescence-prone subpopulation has lower proliferation potential higher...
The signaling pathways whereby glucose and hormonal secretagogues regulate insulin-secretory function, gene transcription, proliferation of pancreatic β-cells are not well defined. We show that in the glucose-responsive β-cell line INS-1, major secretagogue-stimulated converge to activate 44-kDa mitogen-activated protein (MAP) kinase. Thus, glucose-induced insulin secretion was found be associated with a small stimulatory effect on MAP kinase, which synergistically enhanced by increased...
Abstract The potential toxic effects of high extracellular concentrations fatty acids were tested in β(INS-1)-cells cultured the absence serum, a condition known to alter cell survival various systems. This may part mimic situation type 1 or 2 diabetes where β-cells are already insulted by stressful conditions, such as cytokines and oxidative stress. Serum removal caused, over 36-h period, stress an early impairment mitochondrial function, revealed increased superoxide production markedly...
To better understand the action of glucose on fatty acid metabolism in β-cell and link between chronically elevated or acids decompensation adipogenic diabetes, we investigated whether regulates peroxisomal proliferator-activated receptor (PPAR) gene expression β-cell. Islets INS(832/13) β-cells exposed to high show a 60–80% reduction PPARα mRNA expression. Oleate, either absence presence glucose, has no effect. The is dose-dependent 6–20 mm range maximal after 6 h. Glucose also causes...
The molecular mechanisms involved in the degradation of individual cellular proteins are probably unique and characteristic. We have investigated rat liver glyceraldehyde-3-phosphate dehydrogenase, an abundant cytosolic enzyme glycolytic pathway. Immunoblot analysis isolated lysosomes from rats treated with lysosomal inhibitors show that this protein is degraded, at least part, by a This pathway was further incubating cell-free system, followed proteolysis measurements, sodium dodecyl...
Chronic exposure of pancreatic beta-cells to high glucose has pleiotropic action on beta-cell function. In particular, it induces key glycolytic genes, promotes glycogen deposition, and causes proliferation altered insulin secretion characterized by sensitization low glucose. Postglycolytic events, in anaplerosis lipid signaling, are thought be implicated activation To understand the biochemical nature adaptive process hyperglycemia, we studied regulation lipogenic genes line INS-1. A 3-day...
Fatty acids are important metabolic substrates for the pancreatic β-cell, and long term exposure of islets to elevated concentrations fatty results in an alteration glucose-induced insulin secretion. Previous work suggested that exaggerated acid oxidation may be implicated this process by a mechanism requiring changes enzyme expression. We have therefore studied regulation carnitine palmitoyltransferase I (CPT I) gene expression β-cell line INS-1 since catalyzes limiting step various...
To better understand the link between fatty acid signaling and pleiotropic effects of acids in pancreatic beta-cell, we investigated whether regulate immediate-early response genes (IEGs) coding for transcription factors implicated cell proliferation, differentiation, apoptosis. Palmitate oleate, but not long-chain polyunsaturated acids, caused a pronounced accumulation c-fos nur-77 mRNAs beta-cells (INS cells) to an extent similar that produced by protein kinase C (PKC) activator phorbol...
A metabolic model of fuel sensing has been proposed in which malonyl-CoA and long-chain acyl-CoA esters may act as coupling factors nutrient-induced insulin release (Prentki M, Vischer S, Glennon MC, Regazzi R, Deeney J, Corkey BE: Malonyl-CoA long chain secretion. J Biol Chem 267:5802–5810, 1992). To gain further insight into the control content islet tissue, we have studied short- long-term regulation acetyl-CoA carboxylase (ACC) fatty acid synthase (FAS) β-cell. These enzymes catalyze...
Chronic elevation in glucose has pleiotropic effects on the pancreatic β-cell including a high rate of insulin secretion at low glucose, hypertrophy, and hyperplasia. These actions are expected to be associated with modulation expression number glucose-regulated genes that need identified. To further investigate molecular mechanisms implicated these adaptation processes hyperglycemia, we have studied regulation encoding key glycolytic enzymes glucose-responsive line INS-1. Glucose (from 5 25...
Acetyl-CoA carboxylase (ACC) catalyzes the production of malonyl-CoA which may act as a metabolic coupling factor in nutrient-induced insulin release. We have studied long term regulation ACC by nutrients using cell line INS-1. Glucose, from 5 to 20 mM, elicited 15-fold increase mRNA. The effect was detected after 4 h and reached maximum 24 h. protein accumulation followed that mRNA, glucose did not modify half-life transcript. Glucose caused dose-dependent rise 6-phosphate content INS-1...
Elevated glucose and saturated fatty acids synergize in inducing apoptosis INS832/13 cells human islet cells. In order to gain insight into the molecular mechanism(s) of glucolipotoxicity (Gltox), gene profiling metabolic analyses were performed cultured at 5 or 20 mm absence presence palmitate. Expression changes observed for transcripts involved mitochondrial, lipid, metabolism. At 24 h after Gltox, increased expression lipid partitioning genes suggested a promotion acid esterification...
Plant-derived polyphenols have shown potential to alleviate obesity-related pathologies by a multi-targeted mechanism in animal models and human intervention studies.