We present spatial light interference microscopy (SLIM) as a new optical technique, capable of measuring nanoscale structures and dynamics in live cells via interferometry.SLIM combines two classic ideas imaging: Zernike's phase contrast microscopy, which renders high intensity images transparent specimens, Gabor's holography, where the information from object is recorded.Thus, SLIM reveals intrinsic cell and, addition, quantitative path-length maps across sample.The resulting topographic...

The characterization of physical properties cells such as their mass and stiffness has been great interest can have profound implications in cell biology, tissue engineering, cancer, disease research. For example, the direct dependence growth rate on for individual adherent human elucidate mechanisms underlying cycle progression. Here we develop an array micro-electro-mechanical systems (MEMS) resonant sensors that be used to directly measure biophysical properties, mass, single cells....

Journal Article Patterning the differentiation of C2C12 skeletal myoblasts Get access Piyush Bajaj, Bajaj Department Bioengineering, University Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Fax: +1 217 244-6375; Tel: 333-30972000 Micro and Nanotechnology Laboratory, 208 North Wright Street, USA Search for other works by this author on: Oxford Academic Google Scholar Bobby Reddy, Jr., Jr. 2000 USADepartment Electrical Computer Engineering, Larry Millet, Millet Chunan Wei, Wei Pinar...

Microfluidic devices have been used to study high-density cultures of many cell types. Because cell-to-cell signaling is local, however, there exists a need develop culture systems that sustain small numbers neurons and enable analyses the microenvironments. Such are hard maintain in stable form, it difficult prevent death when using primary mammalian neurons. We demonstrate postnatal hippocampal from rat can be cultured at low densities within nanoliter-volume microdevices fabricated...

Wiring the nervous system relies on interplay of intrinsic and extrinsic signaling molecules that control neurite extension, neuronal polarity, process maturation experience-dependent refinement. Extrinsic signals establish enrich neuron–neuron interactions during development. Understanding how such cues direct neurons to neural connections in vitro will facilitate development organized networks for investigating function networks. Producing ordered with defined connectivity presents special...

Adipose tissue is an active endocrine organ that produces a variety of secretory factors involved in the initiation angiogenic processes. The bioactive peptide apelin endogenous ligand G protein-coupled receptor, APJ. Here we investigated potential role and its APJ, responses human endothelial cells development functional vascular network model adipose mice. Treatment umbilical vein with dose-dependently increased responses, including cell migration, proliferation, Matrigel(R) capillary...

We used quantitative phase imaging to measure the dispersion relation, i.e. decay rate vs. spatial mode, associated with mass transport in live cells. This approach applies equally well both discrete and continuous distributions without need for particle tracking. From quadratic experimental curve specific diffusion, we extracted diffusion coefficient as only fitting parameter. The linear portion of relation reveals deterministic component intracellular transport. Our data show a universal...

We present spatial light interference tomography (SLIT), a labelfree method for 3D imaging of transparent structures such as live cells.SLIT uses the principle interferometric with broadband fields and combines optical gating due to micron-scale coherence length that high numerical aperture objective lens.Measuring phase shift map associated object it is translated through focus provides full information about distribution refractive index.Using reconstruction algorithm based on Born...

We developed an interferometric microscopy technique, referred to as Jones phase microscopy, capable of extracting the spatially resolved polarization matrix associated with transparent and anisotropic samples. This is a generalization quantitative imaging, which recovered from one diagonal element measured matrix. The principle technique demonstrated measurements liquid crystal spatial light modulator potential for live cell imaging experiments on neurons in culture.

Customizable 3-D fluidic bridges enable low-volume, leak-free connections between multiple masters for integrated point-of-care protein purification strategies in microfluidics.

Analysis of cell-to-cell variation can further the understanding intracellular processes and role individual cell function within a larger population. The ability to precisely lyse single cells be used release cellular components resolve heterogeneity that might obscured when whole populations are examined. We report method position on silicon nanowire nanoribbon biological field effect transistors. In this study, HT-29 cancer were positioned top transistors by manipulating magnetic beads...

We identified two poplar (Populus sp.)-associated microbes, the fungus, Mortierella elongata strain AG77, and bacterium, Burkholderia BT03, that mutually promote each other's growth. Using culture assays in concert with a novel microfluidic device to generate time-lapse videos, we found growth specific media differing pH pre-conditioned by microbial led increased fungal bacterial rates. Coupling microfluidics comparative metabolomics data results indicated observed stimulation involves...

This paper presents the design and application of microcantilever heaters for biochemical applications. Thermal lysis biological cells was demonstrated as a specific example. The heaters, fabricated from selectively doped single crystal silicon, provide local resistive heating with highly uniform temperature distribution across cantilevers. Very importantly, were coated layer 100 nm thick electrically insulating ultrananocrystalline diamond (UNCD) used cell immobilization on cantilever...

Spatial light interference microscopy (SLIM) is a novel method developed in our laboratory that provides quantitative phase images of transparent structures with 0.3 nm spatial and 0.03 temporal accuracy owing to the white illumination its common path interferometric geometry. We exploit these features demonstrate SLIM's ability perform topography at single atomic layer graphene. Further, using decoupling procedure we for cylindrical structures, extract axially averaged refractive index...

We show that applying the Laplace operator to a speckle-free quantitative phase image reveals an unprecedented level of detail in cell structure, without gradient artifacts associated with differential interference contrast microscopy, or photobleaching and phototoxicity limitations common fluorescence microscopy. This method, referred as is efficient tool for tracking vesicles organelles living cells. The principle demonstrated by cardiomyocytes neurites hippocampal neurons, which our...

Abstract We present phase correlation imaging (PCI) as a novel approach to study cell dynamics in spatially-resolved manner. PCI relies on quantitative time-lapse data and, such, functions label-free mode, without the limitations associated with exogenous markers. The time map outputted informs of intracellular mass transport. Specifically, we show that can extract quantitatively diffusion coefficient live cells, well standard Brownian particles. Due its high sensitivity transport, be...

The mammalian supraoptic nucleus (SON) is a neuroendocrine center in the brain regulating variety of physiological functions. Within SON, peptidergic magnocellular neurons that project to neurohypophysis (posterior pituitary) are involved controlling osmotic balance, lactation, and parturition, partly through secretion signaling peptides such as oxytocin vasopressin into blood. An improved understanding SON activity function requires identification characterization used by SON. Here,...

Abstract Phosphorylation of the histone protein H2AX to form γ-H2AX foci directly represents DNA double-strand break formation. Traditional detection involves counting individual within nuclei. The novelty this work is application a time-resolved fluorescence assay using dissociation-enhanced lanthanide immunoassay for quantitative measurements γ-H2AX. For comparison, standard was employed and analyzed either by bulk fluorescent or direct BioTek Spot Count algorithm Gen 5 software. Etoposide...

Microfluidic systems are well-suited for studying mixed biological communities improving industrial processes of fermentation, biofuel production, and pharmaceutical production. The results which have the potential to resolve underlying mechanisms growth transport in these complex branched living systems. Microfluidics provide controlled environments improved optical access real-time high-resolution imaging studies that allow high-content quantitative analyses. Studying growing structures...

Journal Article Pattern analysis and spatial distribution of neurons in culture Get access Larry J. Millet, Millet Department Electrical Computer Engineering, University Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Fax: 217-244-6375; Tel: 217-333-3097Micro Nanotechnology Laboratory, USANeuroscience Program, USA Search for other works by this author on: Oxford Academic Google Scholar Mitchell B. Collens, Collens Micro USADepartment Bioengineering, George L. W. Perry, Perry School...

We studied the active transport of intracellular components along neuron processes using a new method developed in our laboratory: dispersion-relation phase spectroscopy. This is able to quantitatively map spatially heterogeneous dynamics concentration field cargos at submicron resolution without need for tracking individual components. The results terms density correlation function reveal that decay rate linear wavenumber, which consistent with narrow Lorentzian distribution cargo velocity.