A5081764333- Cancer, Lipids, and Metabolism
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Extracellular vesicles in disease
- Cardiovascular Disease and Adiposity
- Cancer, Hypoxia, and Metabolism
- Cancer, Stress, Anesthesia, and Immune Response
- MicroRNA in disease regulation
- Metabolomics and Mass Spectrometry Studies
- Psoriasis: Treatment and Pathogenesis
- Immunotherapy and Immune Responses
- Esophageal Cancer Research and Treatment
- Mast cells and histamine
- Advanced Proteomics Techniques and Applications
- Sirtuins and Resveratrol in Medicine
- Neuropeptides and Animal Physiology
- Hippo pathway signaling and YAP/TAZ
- Polyamine Metabolism and Applications
- Cutaneous Melanoma Detection and Management
- Vitamin D Research Studies
- Silymarin and Mushroom Poisoning
- Dendrimers and Hyperbranched Polymers
- Plant Virus Research Studies
- Advanced Biosensing Techniques and Applications
- Immune cells in cancer
Institut Toulousain des Maladies Infectieuses et Inflammatoires
2021-2022
Université de Toulouse
2015-2022
Université Toulouse III - Paul Sabatier
2015-2022
Institut de Pharmacologie et de Biologie Structurale
2015-2022
Centre National de la Recherche Scientifique
2014-2022
Inserm
2020-2022
Centre de Physiopathologie de Toulouse-Purpan
2020
Laboratoire de Physique des 2 Infinis Irène Joliot-Curie
2015
Sorbonne Université
2014
Endocrine Society
2008
Abstract Malignant progression results from a dynamic cross-talk between stromal and cancer cells. Recent evidence suggests that this is mediated to significant extent by exosomes, nanovesicles secreted most cell types which allow the transfer of proteins, lipids, nucleic acids Adipocytes are major component several tumor microenvironments, including invasive melanoma, where cells have migrated adipocyte-rich hypodermic layer skin. We show adipocytes secrete exosomes in abundance, then taken...
Summary Exosomes are important mediators in cell‐to‐cell communication and, recently, their role melanoma progression has been brought to light. Here, we characterized exosomes secreted by seven cell lines with varying degrees of aggressivity. Extensive proteomic analysis confirmed the presence characteristic exosomal markers as well melanoma‐specific antigens and oncogenic proteins. Importantly, protein composition differed among from different lines. aggressive cells contained specific...
Abstract Fibrosis is a hallmark of human white adipose tissue ( WAT ) during obesity‐induced chronic inflammation. The functional impact increased interstitial fibrosis (peri‐adipocyte fibrosis) on adjacent adipocytes remains unknown. Here we developed novel in vitro 3D culture system which and decellularized material dMAT from obese subjects are embedded peptide hydrogel. When cultured with , showed decreased lipolysis adipokine secretion expression/production cytokines IL ‐6, G‐ CSF...
Clinical studies suggest that obesity, in addition to promoting breast cancer aggressiveness, is associated with a decrease chemotherapy efficacy, although the mechanisms involved remain elusive. As one of main treatments for aggressive or metastatic cancer, we investigated whether adipocytes can mediate resistance doxorubicin (DOX), drugs used treat and associated. We coculture system grow cells vitro differentiated as well primary mammary isolated from lean obese patients. Drug cellular...
Abstract Prostate gland is surrounded by periprostatic adipose tissue (PPAT), which increasingly believed to play a paracrine role in prostate cancer progression. Our previous work demonstrates that adipocytes promote homing of cells PPAT and this effect upregulated obesity. Here, we show once tumor have invaded (mimicked an vitro model coculture), they establish bidirectional crosstalk with adipocytes, promotes cell invasion. Indeed, induce adipocyte lipolysis the free fatty acids (FFA)...
Cold Atmospheric Plasma (CAP) is an emerging technology with great potential for biomedical applications such as sterilizing equipment and antitumor strategies. CAP has also been shown to improve skin wound healing in vivo, but the biological mechanisms involved are not well known. Our study assessed a possible effect of direct helium jet treatment on keratinocytes, both immortalized N/TERT-1 human cell line primary keratinocytes obtained from samples. The cells were covered 200 µL phosphate...
Pituitary adenylate cyclase-activating peptide (PACAP), a cAMP-activating agent, is highly expressed in the hypothalamus during period when many neuroendocrine cells become differentiated from neural stem (NSCs). Activation of cAMP system rat hypothalamic NSCs these into beta-endorphin (BEP)-producing neurons culture. When vitro were transplanted paraventricular nucleus (PVN) an adult rat, they integrated well with surrounding and produced BEP its precursor gene product, proopiomelanocortin...
Dendrimers are nanosized, arborescent macromolecules synthesized in a stepwise fashion with attractive degrees of functionality and structure definition. This is one the reasons why they widely used for biomedical applications. Previously, we have shown that poly(phosphorhydrazone) (PPH) dendrimer capped anionic azabisphosphonate groups (so-called ABP dendrimer) has immuno-modulatory anti-inflammatory properties towards human immune cells vitro. Thereafter, promising therapeutic efficacy to...
Abstract Extracellular vesicles are emerging key actors in adipocyte communication. Notably, small extracellular shed by adipocytes promote melanoma aggressiveness through fatty acid oxidation, with a heightened effect obesity. However, the vesicular and cellular processes involved remain largely unknown. Here, we elucidate mechanisms linking to metabolic remodeling cell migration. We show that stimulate oxidation providing both enzymes substrates. In obesity, of depends on increased...
<div>Abstract<p>Malignant progression results from a dynamic cross-talk between stromal and cancer cells. Recent evidence suggests that this is mediated to significant extent by exosomes, nanovesicles secreted most cell types which allow the transfer of proteins, lipids, nucleic acids Adipocytes are major component several tumor microenvironments, including invasive melanoma, where cells have migrated adipocyte-rich hypodermic layer skin. We show adipocytes secrete exosomes in...
<p>Supplementary material and methods</p>
<p>1) Adipocyte exosomes promote melanoma migration but have no effect on proliferation; 2) Preadipocytes secrete less than adipocytes, and these behavior; 3) prostate tumor cell their 4) All common exosomal markers are present in the adipocyte exoproteome; 5) increase FAO enzymes SKMEL28 cells; 6) lactate production; 7) dependent 8) Primary adipocytes lean obese conditions which cancer migration</p>
<p>Supplementary material and methods</p>
<p>1) Adipocyte exosomes promote melanoma migration but have no effect on proliferation; 2) Preadipocytes secrete less than adipocytes, and these behavior; 3) prostate tumor cell their 4) All common exosomal markers are present in the adipocyte exoproteome; 5) increase FAO enzymes SKMEL28 cells; 6) lactate production; 7) dependent 8) Primary adipocytes lean obese conditions which cancer migration</p>
<div>Abstract<p>Prostate gland is surrounded by periprostatic adipose tissue (PPAT), which increasingly believed to play a paracrine role in prostate cancer progression. Our previous work demonstrates that adipocytes promote homing of cells PPAT and this effect upregulated obesity. Here, we show once tumor have invaded (mimicked an <i>in vitro</i> model coculture), they establish bidirectional crosstalk with adipocytes, promotes cell invasion. Indeed, induce adipocyte...
<p>Supplementary Figure S1. Coculture with preadipocytes has no effect on cancer cell invasion and coculture mature adipocytes prostate number. Supplementary S2. Adrenergic receptors do not control adipocyte lipolysis in response to tumor secretions. S3. The metabolic remodeling towards uncoupled FAO observed cocultivated cells is involved the increase induced by adipocytes. S4. ROS positively regulate invasion. S5. 10mM NAC 10μM DPI affect PC3 viability. S6. palmitate stimulate...
<div>Abstract<p>Prostate gland is surrounded by periprostatic adipose tissue (PPAT), which increasingly believed to play a paracrine role in prostate cancer progression. Our previous work demonstrates that adipocytes promote homing of cells PPAT and this effect upregulated obesity. Here, we show once tumor have invaded (mimicked an <i>in vitro</i> model coculture), they establish bidirectional crosstalk with adipocytes, promotes cell invasion. Indeed, induce adipocyte...