In order to identify aqueous species formed in Plasma activated media (PAM), quantitative investigations of reactive oxygen and nitrogen (ROS, RNS) were performed compared Milli-Q water culture without with Fetal Calf Serum. Electron paramagnetic resonance, fluorometric colorimetric analysis used quantify free radicals generated by helium plasma jet these liquids. Results clearly show the formation ROS such as hydroxyl radical, superoxide anion radical singlet micromolar range...

Abstract This work investigates the regionalized antiproliferative effects of plasma-activated medium (PAM) on colon adenocarcinoma multicellular tumor spheroid (MCTS), a model that mimics 3D organization and regionalization microtumor region. PAM was generated by dielectric barrier plasma jet setup crossed helium carrier gas. MCTS were transferred in at various times after exposure up to 48 hours effect growth DNA damage evaluated. We report impact duration delay before transfer damage....

This work is devoted to fluid modeling based on experimental investigations of a classical setup low-temperature plasma jet. The latter generated at atmospheric pressure using quartz tube small diameter crossed by helium gas flow and surrounded an electrode system powered mono-polar high-voltage pulse. streamer-like behavior the fast bullets or ionization waves launched in ambient air for every pulse, already emphasized literature from analytical considerations recent preliminary models,...

Two plasma devices at atmospheric pressure (air dielectric barrier discharge and helium jet) have been used to study the early germination of Arabidopsis thaliana seeds during first days. Then, activated waters are later stage plant development growth until 42 The effects on both testa endospserm ruptures significant in case air due its higher energy efficiency producing reactive oxygen species than plasma. latter has shown distinct only for rupture. Analysis stimulations based specific...

The study by Gross et al [...]

This paper is mainly devoted to the comparison between calculation and experimental results of primary secondary streamer development in a point-to-plane positive corona discharge dry air at atmospheric pressure. The qualitative agreement based on hydrodynamics approximation shows that O radical produced which good with recent literature measurements using TALIF diagnostics. However, production yield (in terms radicals per energy injected) more efficient than one. main characteristics are...

In this paper we explore the ability of thermal analysis to check elastin and collagen integrity in different biomaterial applications. Differential Scanning Calorimetry (DSC) has been used analyze first second order transitions biological macromolecules hydrated dehydrated state. First, report characterization control cardiovascular tissues such as pericardium, aortic wall valvular leaflet. Their properties are compared pure collagen. Second, present results obtained on two rich tissues:...

Biomedical applications of low-temperature plasmas are growing interest, especially in the field plasma-induced anti-tumor effects. The present work is aimed at investigating regionalized antiproliferative effects on a multicellular tumor spheroid (MCTS), model that mimics 3D organization and regionalization microtumor region. We report plasma jet, using helium flow open air, inhibits HCT116 colon carcinoma MCTS growth dose-dependent manner. This inhibition associated with loss Ki67,...

Cold atmospheric plasma-exposed culture medium may efficiently kill cancer cells in vitro. Due to the complexity of obtained after plasma exposure, less complex physiological liquids, such as saline solutions and buffers, are gathering momentum. Among plethora reactive oxygen nitrogen species (RONS) that produced these plasma-activated hydrogen peroxide, nitrite nitrate appear be mainly responsible for cytotoxic genotoxic effects. Here, we evaluated anti-cancer potential phosphate-buffered...

Abstract In order to investigate the effects of low temperature plasmas on germination Arabidopsis thaliana seeds, a dielectric barrier discharge device generating plasma in ambient air was used. To highlight different seed surface, saline and osmotic stresses were considered case reference Col-0 seeds two further coat mutants gl2 gpat5 better analyse surface changes their consequences germination. The GL2 gene encode transcription factor controlling balance between biosynthesis fatty acids...

Abstract Numerous studies have reported cold atmospheric plasma cytotoxic activities in various cancer cell lines, either by direct exposure to non-thermal or indirectly activating a medium (plasma-activated medium, PAM) prior treatment. We suggested the use of vitro 3D tumor model spheroids determine potential PAM for therapy at tissue scale, especially human tissue. This work aimed better understand effect on colorectal describing -induced-cell death kinetics and associated mechanisms...

Abstract Cold atmospheric plasma and more recently, plasma-activated liquids (culture media, water or buffered solutions previously exposed to plasma), are gathering momentum in cancer cells treatment. Nevertheless, vitro tests show that this novel approach is sometimes less efficient than expected. We here evaluate the mechanisms of action PBS suggest use electropermeabilization (EP) combination with phosphate-buffered saline (PBS), order potentiate cytotoxic effect activated liquid. Human...

The experimental work reported here is devoted to the temporal behaviour of a mono-filamentary dielectric barrier discharge in pure argon, from 100 700 Torr. A sinusoidal voltage supply used, its frequency ranging 10 90 kHz. An anode avalanche followed by cathode streamer as well spatial stability micro-discharge clearly seen successive 3-ns snapshots visible range. Near cathode, diameter about 0.2 mm, at 400 electrical characteristics are also evaluated, particular breakdown and energy...

Abstract This work is devoted to the evaluation of efficiency a new low-temperature plasma jet driven in ambient air by dc-corona discharge inactivate adherent cells and biofilms Gram-positive bacteria. The selected microorganisms were lactic acid bacteria, Weissella confusa strain which has particularity excrete polysaccharide polymer (dextran) when sucrose present. Both treated with for different exposure times. antimicrobial was tested against 48 h-old grown or without sucrose. Bacterial...

The aim of this work is to investigate the inhibitory effect Plasma Activated Medium (PAM) on Head and Neck cancerous cells (FaDu). response FaDu in monolayer cultures Multi Cellular Tumor Spheroids (MCTS) after treatment with different PAMs will be compared.Head squamous cell carcinoma a widespread cancer that responds poorly anticancer treatments such as chemotherapy radiotherapy. Nowadays there growing interest cold plasmas their applications therapy.A homemade helium plasma jet used...

Cold Atmospheric Plasma (CAP) is an emerging technology with great potential for biomedical applications such as sterilizing equipment and antitumor strategies. CAP has also been shown to improve skin wound healing in vivo, but the biological mechanisms involved are not well known. Our study assessed a possible effect of direct helium jet treatment on keratinocytes, both immortalized N/TERT-1 human cell line primary keratinocytes obtained from samples. The cells were covered 200 µL phosphate...

In this work we experimentally examine the characteristics of a dielectric barrier discharge reactor driven by two distinct pulsed high voltage (HV) generators. Firstly, operation under HV generator delivering positive pulses with short (tenths ns) rise and fall times in low (up to 100 Hz) repetition frequency is illustrated. Then DBD obtain use bipolar larger range (several tenths kHz) having microsecond scale depicted. unipolar excitation regime discharges per pulse occur: first one during...

This paper presents an experimental analysis of the electrical behavior positive point-plane corona discharges. The current, streamer velocity, mean discharge frequency, and current-voltage characteristic are studied, firstly in synthetic air as a function parameters such gap distance tip radius. Different diagnostics used order to better understand development well dependence its characteristics on previous listed parameters. Then influence gas mixture (several proportions N2 O2 with or...

This work is devoted to the analysis of experimental results obtained in dry air at atmospheric pressure a positive point-to-plane corona discharge under pulsed applied voltage cases anodic mono- and multi-tips. In mono-tip case, peak current analysed as function several parameters such magnitude, frequency duration gap distance. The variation correlated with ozone production. Then multi-tip electrical behaviour distance between two contiguous tips tip number order highlight region creation...